Abstract: The primers were designed according to the conservative domain of ETR1 cDNA from other heterogeneous plants. The fragments of 797 bp cDNA , which encoding 265 amino acid residues were amplified through RT-PCR from petals of cut roses ‘Texas’and ‘Viraldi’with different vase lives. Sequence analyses show that the nucleotides of insert fragments in recombinants of ‘Texas’are completely identical ; which named
pRT-ETR1. Sequence homologies of nucleotide and deduced amino acid residues between two kinds of recombination of ‘Viraldi’are 85. 2 % and 92. 5 % respectively , the two kinds of recombination were named pRV-ETR1-V4 and pRV-ETR1-V5. The nucleotide sequence of pRV-ETR1-V5 is 99 % of homology with that of ‘Texas’. Sequence homologies of nucleotide and deduced amino acid residues between pRV-ETR1-V4 and those of
‘Texas’are 85. 0 % and 92. 5 % respectively. Compared with other heterogeneous high plants , all of the above fragments of ETR1 cDNA of cut roses are highly homologous with other high plants , such as peach , apple ,
Arabidopsis and geranium , and homology of amino residues are all beyond 90 %.

Key words: Cut roses, ETR1, Cloning, Sequence analysis