Studies on the Changes of DNA Methylation Level, GA Content and Protein inNon-heading Chinese Cabbage during Vernalization

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (4): 353-357.

• 研究论文 • Previous Articles Next Articles

Studies on the Changes of DNA Methylation Level, GA Content and Protein inNon-heading Chinese Cabbage during Vernalization

Li Meilan^1,3, Wang Qiaomei¹, Zhu Zhujun¹, and Zeng Guangwen²

(¹ Horticultural Department , Zhejiang University , Hangzhou 310029 , China ;　² Biological Science Department , Life Science College , Zhejiang University , Hangzhou 310029 , China ;　³ College of Horticulture , Shanxi Agricultural University , Taigu 030801 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

Abstract

Abstract: With common variety‘Youdonger’as experimental material , the effects of low temperature on flowering of non-heading Chinese cabbage were studied and the changes of DNA methylation level , gibberellin (GA) content and protein varieties were also researched during vernalization of germinated seeds and seedlings. The results showed that either germinated seeds or seedlings could be induced to flower after 4 ℃cold treatment for 30 days. After vernalization , DNA methylation level in shoot apices under both treatment condition was decreased comparing with control plants , but GA content was increased to 2 - 3 times of control’s. A specific protein with a
molecular weight of 58 kD was induced during vernalization but an other kind of protein disappeared. According to the results mentioned above , it implied that vernalization induced flowering of plants by either decreasing DNA
methylation level or increasing GA content in shoot apical tissues.

Key words: Chinese cabbage, Flowering, DNA methylation, Gibberellins, Proteins

CLC Number:

S 634

Li Meilan;Wang Qiaomei;Zhu Zhujun;and Zeng Guangwen. Studies on the Changes of DNA Methylation Level, GA Content and Protein inNon-heading Chinese Cabbage during Vernalization[J]. ACTA HORTICULTURAE SINICA, 2002, 29(4): 353-357.

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Studies on the Changes of DNA Methylation Level, GA Content and Protein inNon-heading Chinese Cabbage during Vernalization

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