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ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (4): 348-352.

• 研究论文 • Previous Articles     Next Articles

Plant Regeneration from Cotyledonary Explants of Chinese Cabbage Cultured in Vitro

Zhang Fenglan1, Takahata Yoshihito2, and Xu Jiabing1   

  1. (1 Beijing Vegetable Research Centre, Beijing 100089, China; 2Faculty of Agriculture, Iwate University , Morioka 020- 8550, Japan)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

Abstract: Effect of hormone combinat ions, AgNO3 and agar concentrat ion on shoot and plant regeneration from cotyledonary explants of Chinese cabbage was described. Maximum shoot regeneration was obtained in MS medium containing 5 mg/ L BA, 0. 5mg/ L NAA, 2 mg/L AgNO3 and solidified with 1. 2 %- 1. 6 % agar. Ethylene production from cultured explants seemed to play important role in shoot regenerat ion. Explants of cultivars with high regenerat ion frequency or if cultured on the medium solidified with higher concentration of agar generally showed low levels of ethylene product ion. However, AgNO3, which also enhanced shoot induction, resulted in an
increase in ethylene production. The possible interaction between ethylene and shoot regenerat ion is discussed.

Key words: Chinese cabbage (Brassica campestris L. ssp. pekinensis), Cotyledon culture, AgNO3, Ethylene

CLC Number: