Obtaining Transgenic Plants of Chinese Cabbage Resistant to Pieris rapae L.with Modified CpTI Gene (sck)

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (3): 224-228.

• 研究论文 • Previous Articles Next Articles

Obtaining Transgenic Plants of Chinese Cabbage Resistant to Pieris rapae L.with Modified CpTI Gene (sck)

Yang Guangdong^{1, 2}, Zhu Zhen³, Li Yan'e², Zhu Zhujun¹, Xiao Guifang³, and Wei Xiaoli³

(¹Department of Horticulture, Zhej iang University, Hangzhou 310029, China; ²Cotton Research I nstitute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China; ³ Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-06-25 Published:2002-06-25

Abstract

Abstract: Modified CpTI (Cowpea Trypsin Inhibitor) gene (sck) was successfully transferred into Chinese cabbage (Brassica campestris L. ssp. pekinensis) varieties ‘GP-11’and ‘Zhongbai 4’ mediated by Agrobacte rium tumefaciens. Kanamycin resistant plants were obtained from cotyledons with petiole. PCR and Southern blot assays confirmed that sck genewas integrated into Chinese cabbage genome DNA. The test about expression of CpTI inhibitory activity indicated that almost every transgenic plant had ability to inhibit bovine trypsin, and non transformed plants had lower activity. Insect􀀁tolerance of the transgenic plants to Pieris rapae L. were observed in
the laboratory and field which showed some transgenic plants had strong resistance to this pest.

Key words: Chinese cabbage, sck, Agrobacterium tumefaciens, CpTI relative capacity, Insect􀀁, tolerance

CLC Number:

Yang Guangdong;;Zhu Zhen;Li Yan'e;Zhu Zhujun;Xiao Guifang;and Wei Xiaoli. Obtaining Transgenic Plants of Chinese Cabbage Resistant to Pieris rapae L.with Modified CpTI Gene (sck)[J]. ACTA HORTICULTURAE SINICA, 2002, 29(3): 224-228.

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Obtaining Transgenic Plants of Chinese Cabbage Resistant to Pieris rapae L.with Modified CpTI Gene (sck)

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