Identification of Aroma Components and Analysis of Expression Characteristics of Terpene Synthase Genes in Chrysanthemum Floral Scent Mutants

doi:10.16420/j.issn.0513-353x.2025-0870

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (2): 513-524.doi: 10.16420/j.issn.0513-353x.2025-0870

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Identification of Aroma Components and Analysis of Expression Characteristics of Terpene Synthase Genes in Chrysanthemum Floral Scent Mutants

HUANG Yawen, HUANG Hongfeng, WANG Zhiling, CHEN Siyu, ZHANG Shiqi, HONG Bo, GU Zhaoyu^**()

Beijing Key Laboratory of Development and Quality Control of Ornamental Horticulture，Department of Ornamental Horticulture，College of Horticulture，China Agricultural University，Beijing 100193，China

Received:2025-09-16 Revised:2025-11-26 Online:2026-02-25 Published:2026-02-12
Contact: GU Zhaoyu

Abstract

Abstract:

Aroma is one of the key quality traits of chrysanthemum. To investigate the differential accumulation of volatile compounds and their biosynthetic mechanisms in chrysanthemum，this study used the chrysanthemum cultivar‘Pink Yan’and its mutant as materials. Techniques including headspace solid-phase microextraction combined with gas chromatography-mass spectrometry（HS-GC-MS），transcriptome sequencing，and quantitative real-time PCR（RT-qPCR）were employed to systematically analyze differences in volatile components and screen related terpene synthase genes. The research showed 56 common components were detected in the volatile substances of the two，mainly terpenes，and 6 and 9 unique components were identified in‘Pink Yan’and mutant. Integrated transcriptome and RT-qPCR analyses revealed six differentially expressed terpene synthase genes，three of which were up-regulated and three down-regulated. Among them，Terpene synthase 7 gene（c25536.graph_c0）showed the highest expression level and significant differential expression，suggesting it may be a key gene regulating the differential accumulation of terpenoid compounds.

Key words: chrysanthemum, aroma compounds, floral scent, terpene synthase, HS-GC-MS

HUANG Yawen, HUANG Hongfeng, WANG Zhiling, CHEN Siyu, ZHANG Shiqi, HONG Bo, GU Zhaoyu. Identification of Aroma Components and Analysis of Expression Characteristics of Terpene Synthase Genes in Chrysanthemum Floral Scent Mutants[J]. Acta Horticulturae Sinica, 2026, 53(2): 513-524.

Figures/Tables 6

References 40

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材料 Material	化合物名称 Compound name	化合物类型 Compound class
粉妍 Pink Yan	芳樟醇 Linalool	萜烯类 Terpene
	1-（6,6-二甲基-2-双环[3.1.0]己-2-烯基）乙酮2-Ethanone,1-（6,6-dimethylbicyclo[3.1.0]hex-2-en-2-yl）-	酮类 Ketone
	7,11-二甲基-3-亚甲基十二烷-1,6,10-三烯 1,6,10-Dodecatriene,7,11-dimethyl-3-methylene-,（E）-	萜烯类 Terpene
	石竹素 Caryophyllene oxide	萜烯类 Terpene
	α-广藿香烯 α-Patchoulene	萜烯类 Terpene
	4（10）-侧柏烯-3-醇乙酸酯 Bicyclo[3.1.0]hexan-3-ol,4-methylene-1-（1-methylethyl）-,acetate	萜烯类 Terpene
突变体 Mutant	2-甲基丁酸-2-甲基丁酯 Butanoic acid,2-methyl-,2-methylbutyl ester	萜烯类 Terpene
	4（10）-侧柏烯-3-醇 Bicyclo[3.1.0]hexan-3-ol,4-methylene-1-（1-methylethyl）-,（1α,3α,5α）-	萜烯类 Terpene
	γ-榄香烯 γ-Elemene	萜烯类 Terpene
	α-衣兰烯 Ylangene	萜烯类 Terpene
	异长叶烯 Isolongifolene	萜烯类 Terpene
	δ-杜松烯 Naphthalene,1,2,3,5,6,8a-hexahydro-4,7-dimethyl-1-（1-methylethyl）-,（1S-cis）-	萜烯类 Terpene
	1,E-8,Z-10-十六碳三烯 1,E-8,Z-10-Hexadecatriene	萜烯类 Terpene
	亚麻醇 Linoleyl alcohol	萜烯类 Terpene
	4-异丙基苯甲醛 Benzaldehyde,4-（1-methylethyl）-	萜烯类 Terpene

材料 Material	化合物名称 Compound name	化合物类型 Compound class
粉妍 Pink Yan	芳樟醇 Linalool	萜烯类 Terpene
	1-（6,6-二甲基-2-双环[3.1.0]己-2-烯基）乙酮2-Ethanone,1-（6,6-dimethylbicyclo[3.1.0]hex-2-en-2-yl）-	酮类 Ketone
	7,11-二甲基-3-亚甲基十二烷-1,6,10-三烯 1,6,10-Dodecatriene,7,11-dimethyl-3-methylene-,（E）-	萜烯类 Terpene
	石竹素 Caryophyllene oxide	萜烯类 Terpene
	α-广藿香烯 α-Patchoulene	萜烯类 Terpene
	4（10）-侧柏烯-3-醇乙酸酯 Bicyclo[3.1.0]hexan-3-ol,4-methylene-1-（1-methylethyl）-,acetate	萜烯类 Terpene
突变体 Mutant	2-甲基丁酸-2-甲基丁酯 Butanoic acid,2-methyl-,2-methylbutyl ester	萜烯类 Terpene
	4（10）-侧柏烯-3-醇 Bicyclo[3.1.0]hexan-3-ol,4-methylene-1-（1-methylethyl）-,（1α,3α,5α）-	萜烯类 Terpene
	γ-榄香烯 γ-Elemene	萜烯类 Terpene
	α-衣兰烯 Ylangene	萜烯类 Terpene
	异长叶烯 Isolongifolene	萜烯类 Terpene
	δ-杜松烯 Naphthalene,1,2,3,5,6,8a-hexahydro-4,7-dimethyl-1-（1-methylethyl）-,（1S-cis）-	萜烯类 Terpene
	1,E-8,Z-10-十六碳三烯 1,E-8,Z-10-Hexadecatriene	萜烯类 Terpene
	亚麻醇 Linoleyl alcohol	萜烯类 Terpene
	4-异丙基苯甲醛 Benzaldehyde,4-（1-methylethyl）-	萜烯类 Terpene

材料 Material	化合物名称 Compound name	化合物类型 Compound class	CAS Chemical abstracts service registry number	化合物浓度/（mg · kg^-1） Odour content	化合物觉察阈值/（mg · kg^-1） Odour threshold value	OAV
粉妍 Pink Yan	芳樟醇Linalool	萜烯类 Terpene	78-70-6	0.163	0.006	27.16666667
粉妍 Pink Yan	石竹素 Caryophyllene oxide	萜烯类 Terpene	1139-30-6	0.097	0.410	0.236585366
突变体 Mutant	2-甲基丁酸，2-甲基丁酯 Butanoic acid,2-methyl-,2- methy- lbutyl ester	萜烯类 Terpene	2445-78-5	0.127	0.075	1.693333333
突变体 Mutant	4-异丙基苯甲醛 Benzaldehyde, 4-(1-methylethyl)-	萜烯类 Terpene	122-03-2	0.067	0.400	0.1675

材料 Material	化合物名称 Compound name	化合物类型 Compound class	CAS Chemical abstracts service registry number	化合物浓度/（mg · kg^-1） Odour content	化合物觉察阈值/（mg · kg^-1） Odour threshold value	OAV
粉妍 Pink Yan	芳樟醇Linalool	萜烯类 Terpene	78-70-6	0.163	0.006	27.16666667
粉妍 Pink Yan	石竹素 Caryophyllene oxide	萜烯类 Terpene	1139-30-6	0.097	0.410	0.236585366
突变体 Mutant	2-甲基丁酸，2-甲基丁酯 Butanoic acid,2-methyl-,2- methy- lbutyl ester	萜烯类 Terpene	2445-78-5	0.127	0.075	1.693333333
突变体 Mutant	4-异丙基苯甲醛 Benzaldehyde, 4-(1-methylethyl)-	萜烯类 Terpene	122-03-2	0.067	0.400	0.1675

Identification of Aroma Components and Analysis of Expression Characteristics of Terpene Synthase Genes in Chrysanthemum Floral Scent Mutants

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