SSR Marker Association Analysis and Fingerprint Construction of Main Traits of Plum Fruit

doi:10.16420/j.issn.0513-353x.2023-0988

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (11): 2495-2509.doi: 10.16420/j.issn.0513-353x.2023-0988

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

SSR Marker Association Analysis and Fingerprint Construction of Main Traits of Plum Fruit

YUE Linqing¹, WU Yichao¹, HE Xingxing¹, XIANG Mingyan¹, WANG Jin², WU Zheng², ZHU Shiguo³, DING Mengqi¹, ZHANG Jian¹, FANG Xiaomei¹^,^**(), YI Zelin¹^,^**()

¹ School of Agriculture and Biotechnology，Southwest University，Chongqing 400715，China
² Fruit Research Institute of Chongqing Academy of Agricultural Sciences，Chongqing 401329，China
³ Chongqing Seed Station，Chongqing 400060，China

Received:2024-03-26 Revised:2024-08-20 Online:2024-12-12 Published:2024-11-25
Contact: FANG Xiaomei, YI Zelin

Abstract

Abstract:

A study on eleven fruit features generated from 102 plum resources was carried out in 2022 and 2023. The coefficient of variation for fruit weight was the highest，while edible ratio and fruit shape indices had lower coefficients. A total of 405 allelic variants were amplified in the test material using 78 pairs of polymorphic primers，with polymorphism information content（PIC）ranging from 0.06 to 0.80 and a mean value of 0.53. An association analysis revealed that 55 SSR markers had highly significant associations with 11 major traits，explaining 4.01%-14.76% of the phenotypic variance. Among the test materials，29 exhibited two or more highly significant associations with traits in two years under GLM and MLM models. From 78 primers，27 pairs of primers were selected to distinguish 102 materials，and the fingerprints of 102 plum resources were constructed.

Key words: plum, SSR, association analysis, fingerprint mapping

YUE Linqing, WU Yichao, HE Xingxing, XIANG Mingyan, WANG Jin, WU Zheng, ZHU Shiguo, DING Mengqi, ZHANG Jian, FANG Xiaomei, YI Zelin. SSR Marker Association Analysis and Fingerprint Construction of Main Traits of Plum Fruit[J]. Acta Horticulturae Sinica, 2024, 51(11): 2495-2509.

Figures/Tables 12

References 26

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编号 No.	引物名 Primer name	正向引物序列（5′-3′） Forward primer sequence	反向引物序列（5′-3′） Reverse primer sequence
1	BPPCT001	AATTCCCAAAGGATGTGTATGAG	CAGGTGAATGAGCCAAAGC
2	BPPCT002	TCGACAGCTTGATCTTGACC	CAATGCCTACGGAGATAAAAGAC
3	BPPCT004	CTGAGTGATCCATTTGCAGG	AGGGCATCTAGACCTCATTGTT
4	BPPCT007	TCATTGCTCGTCATCAGC	CAGATTTCTGAAGTTAGCGGTA
5	BPPCT008	ATGGTGTGTATGGACATGATG	CCTCAACCTAAGACACCTTCA
6	BPPCT009	ATTCGGGTCGAACTCCCT	ACGAGCACTAGAGTAACCCTC
7	BPPCT010	AAAGCACAGCCCATAATGC	GTACTGTTACTGCTGGGAATGC
8	BPPCT012	ACTTCCATTGTCAGGCATCA	GGAGCAACGATGGAGTGC
9	BPPCT017	TTAAGAGTTTGTGATGGGAACC	AAGCATAATTTAGCATAACCAAG
10	BPPCT020	CGTGGATGGTCAAGATGC	ATTGACGTGGACTTACAGGTG
11	BPPCT024	GAGGAATGTGCCTCTTCTGG	CTCCCGTACGCGTTTACC
12	BPPCT025	TCCTGCGTAGAAGAAGGTAGC	CGACATAAAGTCCAAATGGC
13	BPPCT026	ATACCTTTGCCACTTGCG	TGAGTTGGAAGAAAACGTAACA
14	BPPCT028	TCAAGTTAGCTGAGGATCGC	GAGCTTGCCTATGAGAAGACC
15	BPPCT029	GGACGGACAGAAATGAAGGT	CCTTAACCCACGCAACTCC
16	BPPCT030	AATTGTACTTGCCAATGCTAT	CTGCCTTCTGCTCACACC
17	BPPCT032	TTAAGCCACAACATCCATGAT	AATGGTCTAAGGAGCACACG
18	BPPCT033	GTAGCCGGAGCCGTGTAT	CTAGAACCCTATAAACACATGGC
19	BPPCT034	CTACCTGAAATAAGCAGAGCCAT	CAATGGAGAATGGGGTGC
20	BPPCT036	AAGCAAAGTCCATAAAAACGC	GGACGAAGACGCTCCATT
21	BPPCT037	CATGGAAGAGGATCAAGT	CTTGAAGGTAGTGCCAAA
22	BPPCT040	ATGAGGACGTGTCTGAAT	TGCCAAACCCCTCTTATA
23	BPPCT041	CAATAAGGCATTTGGAGG	CAGCCGAACCAAGGAGAC
24	CPDCT016	GGAAACCTGATTAGGGCACTT	GGTCTGCTATACTGACCTAGGATT
25	CPDCT028	TGAACGTTGCACTCCTTCAC	ACCACCACCATAACCACCAT
26	CPDCT045	TGTGGATCAAGAAAGAGAACCA	AGGTGTGCTTGCACATGTTT
27	CPPCT025	CAATTAGCTAGAGAGAATTATTG	GACAAGAAGCAAGTAGTTTG
28	CPPCT1	TGTATCCCCTCCTCGGATAA	GGCATTAATGGATGATGATGAA
29	CPPCT13	AGACGCAGCACCCAAACTAC	CATTACATCACCGCCAACAA
30	CPPCT22	ACTCGTGGAGCAGACCACTT	ATCCAAGCATGCCAAGAAAG
31	CPPCT26	TGCTTTCCACGCACACTG	GCCAAGCATTGCGTCGTT
32	CPPCT34	GCATTTCGAGAGCTGTATTT	GTCTTACGTGCAGCTTCATT
33	CPPCT35	TCGGTTTTTAAAATTCCAAAAGTT	ACCCTTATTTGCACCCAACA
34	CPPCT42	CTACCCATTAGCCACCAAGC	TCCCAATTCGTTGCAATCTT
35	CPSCT004	GCTCTGAAGCTCTGCATTGA	TTTGAAATGGCTATGGAGTACG
36	CPSCT005	CTGCAAGCACTGCGGATCTC	CCCATATTCCCAACCCATTA
37	CPSCT006	ACAAACCAAGCACCGTCTC	GGGCAAATGCTTACCTGTTC
38	CPSCT011	ATTTGGGTTTGCGACTCAAG	ACTCATCCCTTGCCCTTTCT
39	CPSCT012	ACGGGAGACTTTCCCAGAAG	CTTCTCGTTTCCTCCCTCCT
40	CPSCT018	AGGACATGTGGTCCAACCTC	GGGTTCCCCGTTACTTTCAT
41	CPSCT021	GCCACTTCGGCTAAAAGAGA	TCCATATCTCCTCCTGCTTGA
42	CPSCT022	TGTCTGCCTCTCATCTTAACCA	TTCTTGAGCAGCCCATCTTCT
43	CPSCT023	CGGGTTGACTCAGTTCCTTC	ATTTGAGCAGAAGCCCAGAA
44	CPSCT024	TGGGTCGTCTTCTTTATCGTG	CCTCACCAAAACGGTAGTCAG
45	CPSCT026	TCTCACACGCTTTCGTCAAC	AAAAAGCCAAAAGGGGTTGT
46	CPSCT032	CATCATCATCCTCACCCAAA	TGCTGATCCGTGAGATCTTG
47	CPSCT033	TCCTCATTTGAGTGTTGTGGA	TGCCCAATTTGAAAACTTTGT
48	CPSCT034	AGGTGGACAATAGCCGTGAT	TTTCCAGACCCTGAGAAAGC
49	CPSCT036	TCGAAGACAGACCAGACAAAGA	TTGCCTTATGCCGGTAATTT
50	CPSCT039	GCCGCAACTCGTAAGGAATA	TCCACCGTTGATTACCCTTC
51	EPDCU5100	CTCTTCTCGCCTCCCAATTT	TGCTTAGCCCTGGGTACAAG
52	GS1	CCACCTGCAACCTTTTTCCC	ATGTTTCCACTGCCTGCCTT
53	PceGA025	GTTGTGTTTACTTTTTTCTTAACGG	GTATCACAAGTGAGAACATAAGAGG
54	PchcmS1	GCAATTCGAGCTGTATTTCAGATG	CAGTTGGCGGCTATCATGTCTTAC
55	PchcmS2	GTTACACCTCTGTCACA	CTTGGCTGGCATTCCTA
56	PchcmS4	AGGGTCGTCTCTTTGAC	CTTCGTTTCAAGGCCTG
57	PchgmS2	CTCACGCTATTTCTCGG	CCTCGACGAAGAGCTCG
58	PchgmS4	GCTAATGAGTTCAGTGTCTACACTC	AATCATAACATCATTCAGCCACTGC
59	PchgmS6	ATCTTCACAACCCTAATG	GTTGAGGCAAAAGACTTC
60	PF1	CATTGTTCATGGGAGGAATT	AGAACATTCCTAAAGGAGCA
61	PGS1.21	CACGAGTGCCAATCCCACC	TTCTTCCGGAAGACCTTGGC
62	PGS1.24	CCCTGGTGTTCTGCTCTCTC	CATCCACAAATGGGAAGCAT
63	PS0lH03	GTAAATGAGTGCCTGCGTGT	TGCGAGAGTTGTGATTGATG
64	PS08E08	TGAGGAGCATAATGACAGT	TCACCATGTGTCATACT
65	PS12A02	CCCAATGAACAACTGCAT	CATATCAATCACTGGGAT
66	SsrPaCITA5	GCCACCAATGGTTCTTCC	AGCACCAGATGCACCTGA
67	UDP96-003	TTGCTCAAAAGTGTCGTTGC	ACACGTAGTGCAACACTGGC
68	UDP96-005	GTAACGCTCGCTACCACAAA	CCTGCATATCACCACCCAG
69	UDP96-008	TTGTACACACCCTCAGCCTG	TGCTGAGGTTCAGGTGAGTG
70	UDP98-022	CTAGTTGTGCACACTCACGC	GTCGCAGGAACAGTAAGCCT
71	UDP98-024	CCTTGATGCATAATCAAACAGC	GGACACACTGGCATGTGAAG
72	UDP98-025	GGGAGGTTACTATGCCATGAAT	CGCAGACATGTAGGACCTC
73	UDP98-405	ACGTGATGAACTGACACCCA	GAGTCTTTGCTCTGCCATCC
74	UDP98-406	TCGGAAACTGGTAGTATGAACAGA	ATGGGTCGTATGCACAGTCA
75	UDP98-409	GCTGATGGGTTTTATGGTTTTC	CGGACTCTTATCCTCTATCAACA
76	UDP98-410	AATTTACCTATCAGCCTCAAA	TTTATGCAGTTTACAGACCG
77	UDP98-412	AGGGAAAGTTTCTGCTGCAC	GCTGAAGACGACGATGATGA
78	UDP98-416	TTTTCTCAGCAGCCAAACAA	ATGTTTCGTGCTTCTGCTCC

编号 No.	引物名 Primer name	正向引物序列（5′-3′） Forward primer sequence	反向引物序列（5′-3′） Reverse primer sequence
1	BPPCT001	AATTCCCAAAGGATGTGTATGAG	CAGGTGAATGAGCCAAAGC
2	BPPCT002	TCGACAGCTTGATCTTGACC	CAATGCCTACGGAGATAAAAGAC
3	BPPCT004	CTGAGTGATCCATTTGCAGG	AGGGCATCTAGACCTCATTGTT
4	BPPCT007	TCATTGCTCGTCATCAGC	CAGATTTCTGAAGTTAGCGGTA
5	BPPCT008	ATGGTGTGTATGGACATGATG	CCTCAACCTAAGACACCTTCA
6	BPPCT009	ATTCGGGTCGAACTCCCT	ACGAGCACTAGAGTAACCCTC
7	BPPCT010	AAAGCACAGCCCATAATGC	GTACTGTTACTGCTGGGAATGC
8	BPPCT012	ACTTCCATTGTCAGGCATCA	GGAGCAACGATGGAGTGC
9	BPPCT017	TTAAGAGTTTGTGATGGGAACC	AAGCATAATTTAGCATAACCAAG
10	BPPCT020	CGTGGATGGTCAAGATGC	ATTGACGTGGACTTACAGGTG
11	BPPCT024	GAGGAATGTGCCTCTTCTGG	CTCCCGTACGCGTTTACC
12	BPPCT025	TCCTGCGTAGAAGAAGGTAGC	CGACATAAAGTCCAAATGGC
13	BPPCT026	ATACCTTTGCCACTTGCG	TGAGTTGGAAGAAAACGTAACA
14	BPPCT028	TCAAGTTAGCTGAGGATCGC	GAGCTTGCCTATGAGAAGACC
15	BPPCT029	GGACGGACAGAAATGAAGGT	CCTTAACCCACGCAACTCC
16	BPPCT030	AATTGTACTTGCCAATGCTAT	CTGCCTTCTGCTCACACC
17	BPPCT032	TTAAGCCACAACATCCATGAT	AATGGTCTAAGGAGCACACG
18	BPPCT033	GTAGCCGGAGCCGTGTAT	CTAGAACCCTATAAACACATGGC
19	BPPCT034	CTACCTGAAATAAGCAGAGCCAT	CAATGGAGAATGGGGTGC
20	BPPCT036	AAGCAAAGTCCATAAAAACGC	GGACGAAGACGCTCCATT
21	BPPCT037	CATGGAAGAGGATCAAGT	CTTGAAGGTAGTGCCAAA
22	BPPCT040	ATGAGGACGTGTCTGAAT	TGCCAAACCCCTCTTATA
23	BPPCT041	CAATAAGGCATTTGGAGG	CAGCCGAACCAAGGAGAC
24	CPDCT016	GGAAACCTGATTAGGGCACTT	GGTCTGCTATACTGACCTAGGATT
25	CPDCT028	TGAACGTTGCACTCCTTCAC	ACCACCACCATAACCACCAT
26	CPDCT045	TGTGGATCAAGAAAGAGAACCA	AGGTGTGCTTGCACATGTTT
27	CPPCT025	CAATTAGCTAGAGAGAATTATTG	GACAAGAAGCAAGTAGTTTG
28	CPPCT1	TGTATCCCCTCCTCGGATAA	GGCATTAATGGATGATGATGAA
29	CPPCT13	AGACGCAGCACCCAAACTAC	CATTACATCACCGCCAACAA
30	CPPCT22	ACTCGTGGAGCAGACCACTT	ATCCAAGCATGCCAAGAAAG
31	CPPCT26	TGCTTTCCACGCACACTG	GCCAAGCATTGCGTCGTT
32	CPPCT34	GCATTTCGAGAGCTGTATTT	GTCTTACGTGCAGCTTCATT
33	CPPCT35	TCGGTTTTTAAAATTCCAAAAGTT	ACCCTTATTTGCACCCAACA
34	CPPCT42	CTACCCATTAGCCACCAAGC	TCCCAATTCGTTGCAATCTT
35	CPSCT004	GCTCTGAAGCTCTGCATTGA	TTTGAAATGGCTATGGAGTACG
36	CPSCT005	CTGCAAGCACTGCGGATCTC	CCCATATTCCCAACCCATTA
37	CPSCT006	ACAAACCAAGCACCGTCTC	GGGCAAATGCTTACCTGTTC
38	CPSCT011	ATTTGGGTTTGCGACTCAAG	ACTCATCCCTTGCCCTTTCT
39	CPSCT012	ACGGGAGACTTTCCCAGAAG	CTTCTCGTTTCCTCCCTCCT
40	CPSCT018	AGGACATGTGGTCCAACCTC	GGGTTCCCCGTTACTTTCAT
41	CPSCT021	GCCACTTCGGCTAAAAGAGA	TCCATATCTCCTCCTGCTTGA
42	CPSCT022	TGTCTGCCTCTCATCTTAACCA	TTCTTGAGCAGCCCATCTTCT
43	CPSCT023	CGGGTTGACTCAGTTCCTTC	ATTTGAGCAGAAGCCCAGAA
44	CPSCT024	TGGGTCGTCTTCTTTATCGTG	CCTCACCAAAACGGTAGTCAG
45	CPSCT026	TCTCACACGCTTTCGTCAAC	AAAAAGCCAAAAGGGGTTGT
46	CPSCT032	CATCATCATCCTCACCCAAA	TGCTGATCCGTGAGATCTTG
47	CPSCT033	TCCTCATTTGAGTGTTGTGGA	TGCCCAATTTGAAAACTTTGT
48	CPSCT034	AGGTGGACAATAGCCGTGAT	TTTCCAGACCCTGAGAAAGC
49	CPSCT036	TCGAAGACAGACCAGACAAAGA	TTGCCTTATGCCGGTAATTT
50	CPSCT039	GCCGCAACTCGTAAGGAATA	TCCACCGTTGATTACCCTTC
51	EPDCU5100	CTCTTCTCGCCTCCCAATTT	TGCTTAGCCCTGGGTACAAG
52	GS1	CCACCTGCAACCTTTTTCCC	ATGTTTCCACTGCCTGCCTT
53	PceGA025	GTTGTGTTTACTTTTTTCTTAACGG	GTATCACAAGTGAGAACATAAGAGG
54	PchcmS1	GCAATTCGAGCTGTATTTCAGATG	CAGTTGGCGGCTATCATGTCTTAC
55	PchcmS2	GTTACACCTCTGTCACA	CTTGGCTGGCATTCCTA
56	PchcmS4	AGGGTCGTCTCTTTGAC	CTTCGTTTCAAGGCCTG
57	PchgmS2	CTCACGCTATTTCTCGG	CCTCGACGAAGAGCTCG
58	PchgmS4	GCTAATGAGTTCAGTGTCTACACTC	AATCATAACATCATTCAGCCACTGC
59	PchgmS6	ATCTTCACAACCCTAATG	GTTGAGGCAAAAGACTTC
60	PF1	CATTGTTCATGGGAGGAATT	AGAACATTCCTAAAGGAGCA
61	PGS1.21	CACGAGTGCCAATCCCACC	TTCTTCCGGAAGACCTTGGC
62	PGS1.24	CCCTGGTGTTCTGCTCTCTC	CATCCACAAATGGGAAGCAT
63	PS0lH03	GTAAATGAGTGCCTGCGTGT	TGCGAGAGTTGTGATTGATG
64	PS08E08	TGAGGAGCATAATGACAGT	TCACCATGTGTCATACT
65	PS12A02	CCCAATGAACAACTGCAT	CATATCAATCACTGGGAT
66	SsrPaCITA5	GCCACCAATGGTTCTTCC	AGCACCAGATGCACCTGA
67	UDP96-003	TTGCTCAAAAGTGTCGTTGC	ACACGTAGTGCAACACTGGC
68	UDP96-005	GTAACGCTCGCTACCACAAA	CCTGCATATCACCACCCAG
69	UDP96-008	TTGTACACACCCTCAGCCTG	TGCTGAGGTTCAGGTGAGTG
70	UDP98-022	CTAGTTGTGCACACTCACGC	GTCGCAGGAACAGTAAGCCT
71	UDP98-024	CCTTGATGCATAATCAAACAGC	GGACACACTGGCATGTGAAG
72	UDP98-025	GGGAGGTTACTATGCCATGAAT	CGCAGACATGTAGGACCTC
73	UDP98-405	ACGTGATGAACTGACACCCA	GAGTCTTTGCTCTGCCATCC
74	UDP98-406	TCGGAAACTGGTAGTATGAACAGA	ATGGGTCGTATGCACAGTCA
75	UDP98-409	GCTGATGGGTTTTATGGTTTTC	CGGACTCTTATCCTCTATCAACA
76	UDP98-410	AATTTACCTATCAGCCTCAAA	TTTATGCAGTTTACAGACCG
77	UDP98-412	AGGGAAAGTTTCTGCTGCAC	GCTGAAGACGACGATGATGA
78	UDP98-416	TTTTCTCAGCAGCCAAACAA	ATGTTTCGTGCTTCTGCTCC

性状 Trait	年份 Year	最大值 Max	最小值 Min	平均值 Mean	极差 Range	标准差 SD	变异系数/% CV	偏度 Skew.	峰度 Kurt.
LD/mm	2022	53.10	27.04	35.61	26.06	6.53	18.33	0.75	-0.21
	2023	54.50	26.54	37.14	27.96	6.17	16.60	0.39	-0.23
TD/mm	2022	53.12	29.90	40.34	23.22	6.56	16.25	0.18	-1.15
	2023	61.81	28.90	41.65	32.91	6.43	15.44	0.15	-0.19
FS	2022	1.08	0.66	0.90	0.42	0.07	8.09	-0.09	0.11
	2023	1.04	0.78	0.89	0.26	0.07	7.32	0.28	-0.80
FW/g	2022	87.05	13.97	35.64	73.08	17.32	48.59	0.78	-0.06
	2023	89.11	13.13	38.40	75.98	16.87	43.92	0.73	0.47
EA/%	2022	0.98	0.93	0.97	0.05	0.01	1.34	-1.12	0.62
	2023	0.99	0.93	0.97	0.06	0.01	1.31	-1.01	0.71
FH/N	2022	19.46	1.72	11.68	17.74	4.57	39.11	-0.50	-0.59
	2023	21.81	0.82	11.12	20.99	5.15	46.33	-0.04	-0.66
TS/%	2022	13.20	6.46	9.63	6.74	1.46	15.20	-0.08	0.03
	2023	13.21	6.32	9.63	6.89	1.53	15.91	-0.11	-0.41
RS/%	2022	9.05	4.23	6.89	4.82	1.09	15.82	-0.30	-0.23
	2023	9.49	3.01	7.04	6.48	1.34	18.99	-0.49	-0.15
VC/（μg · g^-1）	2022	67.6	13.9	38.9	53.7	11.0	28.34	0.11	-0.12
	2023	71.3	9.6	40.4	61.7	13.9	34.48	0.08	-0.32
TSS/%	2022	16.70	8.60	12.61	8.10	1.72	13.62	-0.25	0.02
	2023	16.90	6.60	12.31	10.30	2.13	17.29	-0.30	-0.18
TA/%	2022	1.43	0.49	0.95	0.94	0.18	18.69	-0.07	0.03
	2023	1.71	0.42	0.93	1.29	0.24	25.79	0.38	0.36

性状 Trait	年份 Year	最大值 Max	最小值 Min	平均值 Mean	极差 Range	标准差 SD	变异系数/% CV	偏度 Skew.	峰度 Kurt.
LD/mm	2022	53.10	27.04	35.61	26.06	6.53	18.33	0.75	-0.21
	2023	54.50	26.54	37.14	27.96	6.17	16.60	0.39	-0.23
TD/mm	2022	53.12	29.90	40.34	23.22	6.56	16.25	0.18	-1.15
	2023	61.81	28.90	41.65	32.91	6.43	15.44	0.15	-0.19
FS	2022	1.08	0.66	0.90	0.42	0.07	8.09	-0.09	0.11
	2023	1.04	0.78	0.89	0.26	0.07	7.32	0.28	-0.80
FW/g	2022	87.05	13.97	35.64	73.08	17.32	48.59	0.78	-0.06
	2023	89.11	13.13	38.40	75.98	16.87	43.92	0.73	0.47
EA/%	2022	0.98	0.93	0.97	0.05	0.01	1.34	-1.12	0.62
	2023	0.99	0.93	0.97	0.06	0.01	1.31	-1.01	0.71
FH/N	2022	19.46	1.72	11.68	17.74	4.57	39.11	-0.50	-0.59
	2023	21.81	0.82	11.12	20.99	5.15	46.33	-0.04	-0.66
TS/%	2022	13.20	6.46	9.63	6.74	1.46	15.20	-0.08	0.03
	2023	13.21	6.32	9.63	6.89	1.53	15.91	-0.11	-0.41
RS/%	2022	9.05	4.23	6.89	4.82	1.09	15.82	-0.30	-0.23
	2023	9.49	3.01	7.04	6.48	1.34	18.99	-0.49	-0.15
VC/（μg · g^-1）	2022	67.6	13.9	38.9	53.7	11.0	28.34	0.11	-0.12
	2023	71.3	9.6	40.4	61.7	13.9	34.48	0.08	-0.32
TSS/%	2022	16.70	8.60	12.61	8.10	1.72	13.62	-0.25	0.02
	2023	16.90	6.60	12.31	10.30	2.13	17.29	-0.30	-0.18
TA/%	2022	1.43	0.49	0.95	0.94	0.18	18.69	-0.07	0.03
	2023	1.71	0.42	0.93	1.29	0.24	25.79	0.38	0.36

引物名称 Primer name	条带数 Number of strips	多态性条带数 Polymorp-hic bands	等位基因数 N_a	主要等位基因频率 Major allele frquency	基因多样性 H_e	多态性信息含量 PIC
BPPCT001	8	6	8	0.43	0.76	0.72
BPPCT002	5	3	5	0.81	0.12	0.31
BPPCT004	10	6	7	0.38	0.37	0.74
BPPCT007	15	11	9	0.38	0.95	0.75
BPPCT008	7	4	4	0.40	0.99	0.64
BPPCT009	9	8	9	0.36	0.96	0.71
BPPCT010	6	4	5	0.45	0.89	0.64
BPPCT012	6	4	4	0.40	0.93	0.64
BPPCT017	5	3	4	0.43	0.50	0.65
BPPCT020	9	3	4	0.42	0.35	0.64
BPPCT024	11	7	8	0.42	0.99	0.66
BPPCT025	9	6	7	0.44	0.95	0.58
BPPCT026	9	8	9	0.44	0.93	0.69
BPPCT028	12	7	8	0.37	0.97	0.72
BPPCT029	8	6	7	0.27	0.84	0.78
BPPCT030	7	6	7	0.69	0.11	0.44
BPPCT032	7	2	3	0.80	0.20	0.31
BPPCT033	10	7	5	0.49	0.98	0.55
BPPCT034	8	3	4	0.38	0.87	0.61
BPPCT036	6	5	5	0.49	0.98	0.46
BPPCT037	7	3	5	0.65	0.21	0.51
BPPCT040	7	5	6	0.45	0.78	0.62
BPPCT041	5	3	4	0.70	0.29	0.45
CPDCT016	8	2	4	0.77	0.32	0.34
CPDCT028	4	2	4	0.52	0.64	0.54
CPDCT045	7	3	4	0.51	0.35	0.57
CPPCT025	4	4	4	0.51	0.82	0.54
CPPCT1	8	6	6	0.48	0.79	0.57
CPPCT13	8	6	7	0.26	0.93	0.80
CPPCT22	6	2	4	0.57	0.20	0.51
CPPCT26	7	5	6	0.41	0.91	0.65
CPPCT34	4	4	5	0.56	0.24	0.56
CPPCT35	10	8	9	0.26	0.81	0.78
CPPCT42	8	5	6	0.45	0.99	0.54
CPSCT004	8	4	5	0.38	0.91	0.64
CPSCT005	10	7	6	0.38	0.93	0.73
CPSCT006	10	6	7	0.39	0.91	0.63
CPSCT011	8	6	7	0.36	0.96	0.73
CPSCT012	7	5	6	0.49	0.94	0.59
CPSCT018	10	4	4	0.39	0.95	0.65
CPSCT021	8	5	5	0.47	0.97	0.62
CPSCT022	5	5	6	0.36	0.89	0.71
CPSCT023	6	4	5	0.52	0.85	0.46
CPSCT024	9	3	4	0.41	0.84	0.59
CPSCT026	7	5	6	0.38	0.89	0.69
CPSCT032	6	3	4	0.52	0.72	0.52
CPSCT033	5	4	5	0.46	0.96	0.64
CPSCT034	6	4	4	0.82	0.25	0.29
CPSCT036	8	2	3	0.90	0.20	0.17
CPSCT039	7	5	6	0.42	0.96	0.66
EPDCU5100	7	2	3	0.87	0.11	0.23
GS1	6	5	6	0.44	0.41	0.68
PceGA025	7	3	5	0.53	0.20	0.59
PchcmS1	3	1	3	0.82	0.00	0.26
PchcmS2	2	1	3	0.83	0.00	0.27
PchcmS4	4	2	3	0.95	0.10	0.09
PchgmS2	5	3	4	0.59	0.60	0.47
PchgmS4	6	4	4	0.74	0.30	0.40
PchgmS6	7	5	6	0.33	0.78	0.76
PF1	2	1	2	0.96	0.00	0.07
PGS1.21	5	4	6	0.55	0.39	0.58
PGS1.24	5	2	4	0.78	0.01	0.31
PS0lH03	6	3	5	0.88	0.05	0.21
PS08E08	5	3	4	0.84	0.15	0.27
PS12A02	7	2	3	0.77	0.12	0.31
SsrPaCITA5	4	1	3	0.97	0.00	0.06
UDP96-003	6	3	6	0.48	0.95	0.45
UDP96-005	5	4	5	0.47	0.88	0.53
UDP96-008	6	4	6	0.57	0.22	0.59
UDP98-022	8	1	8	0.46	0.00	0.65
UDP98-024	8	2	4	0.85	0.05	0.25
UDP98-025	6	5	6	0.41	0.87	0.70
UDP98-405	5	3	5	0.77	0.16	0.37
UDP98-406	6	2	4	0.64	0.20	0.51
UDP98-409	11	4	6	0.50	0.21	0.60
UDP98-410	7	2	3	0.87	0.01	0.22
UDP98-412	6	4	5	0.60	0.76	0.49
UDP98-416	2	1	4	0.56	0.43	0.53
平均值Mean	6.88	4.05	5.19	0.55	0.56	0.53

SSR Marker Association Analysis and Fingerprint Construction of Main Traits of Plum Fruit

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性状 Character	关联位点个数 Number of associated sites	被检测到的次数 The number of times it was detected			表型变异解释率范围/% R²
性状 Character	关联位点个数 Number of associated sites	2	3	4	表型变异解释率范围/% R²
LD	7	3	—	1	4.49 ~ 8.50
TD	3	0	2	0	4.69 ~ 7.23
FS	16	1	4	1	5.07 ~ 14.42
FW	11	5	1	0	4.01 ~ 6.67
EA	10	2	—	—	6.03 ~ 13.08
FH	25	4	3	—	5.05 ~ 13.96
TS	12	2	—	1	4.47 ~ 14.76
RS	5	2	—	1	5.39 ~ 10.60
VC	9	3	1	2	5.35 ~ 14.38
TSS	14	7	—	1	5.65 ~ 11.43
TA	5	2	1	1	7.13 ~ 13.32

性状 Character	引物 Primer	变异表型解释率/% R²
性状 Character	引物 Primer	2022GLM	2022MLM	2023GLM	2023MLM
LD	CPSCT012	7.95	8.50	5.34	5.01
	PchgmS2	6.30	6.87
	PchgmS4	6.30	6.59
	BPPCT007			7.14	7.044
TD	CPSCT005	7.23	6.63	5.01
	BPPCT025	5.96	5.97	4.88
FS	CPPCT34	14.42	6.64	12.16
	CPSCT024	13.06	5.79	11.38	7.10
	PchgmS2	9.13	5.16	7.12
	CPSCT018	8.62	5.12	7.04
	GS1	8.49	5.07	7.01
	BPPCT010			13.16	8.52
FW	PchgmS4	5.71	6.67	4.67
	CPPCT13	5.35	6.01
	CPSCT012	5.24	6.25
	EPDCU5100	5.13	5.51
	BPPCT025	5.07		4.71
	PGS1.21			4.01	4.82
EA	CPSCT024	13.08	7.50
	CPPCT35	10.39	9.22
FH	BPPCT007	13.96		12.07	7.73
	PS12A02	11.09		6.47	7.27
	UDP98-025	6.06		7.91	7.24
	PceGA025	9.03	7.27
	BPPCT029	6.90		6.81
	CPPCT13	7.66		5.79
TS	BPPCT032	14.76	8.01
	PGS1.24	7.62	8.02	5.93	4.47
	BPPCT025			10.90	10.90
RS	BPPCT032	10.60	8.48
	CPSCT018	5.39	6.11	8.27	9.06
	BPPCT033			5.36	6.79
VC	UDP98-025	14.32	14.00	13.45	14.38
	PS12A02	7.53	7.26
	CPPCT42	6.20		5.96
	BPPCT026	6.16	9.07	6.03	7.77
	CPPCT26	6.16	8.46	5.46
	BPPCT036	5.64	6.94
TSS	BPPCT032	11.43	7.37
	PchgmS2	10.11	9.43
	CPSCT005	10.02		8.53
	UDP98-409	9.96	7.61	5.79	6.70
	CPPCT35	8.63	7.48
	GS1	7.22		7.99
	CPSCT012	6.37		6.70
	UDP96-008			8.86	9.02
TA	BPPCT009	9.94	9.61
	BPPCT026	8.08	8.04	13.32	1.93
	BPPCT007	7.13		9.53	7.15
	BPPCT002			9.12	7.15

引物 Primer	性状与年份 Character and year	分析模型 Analysis model	R²/%	F检验 F marker
BPPCT001	TSS_2022	GLM	7.09	8.08
BPPCT002	TA_2023	GLM，MLM	7.15 ~ 9.12	6.9 ~ 10.0
BPPCT004	FW_2023，EA_2022，TS_2023	GLM，MLM	7.06 ~ 9.54	7.32 ~ 9.72
BPPCT007	LD_2023，FS_2023，FW_2023，EA_2022，FH_2022， FH_2023，TA_2022，TA2023	GLM，MLM	7.04 ~ 13.96	6.92 ~ 19.77
BPPCT008	FH_2023	GLM	5.68	7.78
BPPCT009	TD_2022，FH_2022，FH_2023，TA_2022	GLM，MLM	4.46 ~ 9.94	7.28 ~ 11.41
BPPCT010	FS_2023，FW_2022，EA_2022	GLM，MLM	4.43 ~ 13.16	7.02 ~ 14.47
BPPCT012	FW_2023	GLM	4.56	7.78
BPPCT025	TD_2022，TD2023，FW2022，FW2023，TS_2023	GLM，MLM	4.71 ~ 10.90	7.30 ~ 14.39
BPPCT026	FS_2023，VC_2022，VC_2023，TA_2022，TA_2023	GLM，MLM	6.03 ~ 13.32	7.79 ~ 15.32
BPPCT029	FH_2022，FH_2023	GLM	6.81 ~ 6.90	8.84 ~ 9.16
BPPCT030	FS_2022，TS_2022	GLM	7.37 ~ 7.94	8.23 ~ 9.96
BPPCT032	TS_2022，RS_2022，TSS_2022	GLM，MLM	7.37 ~ 14.76	7.27 ~ 20.36
BPPCT033	RS_2023	GLM，MLM	5.36 ~ 6.79	7.74 ~ 7.76
BPPCT034	FH_2023	GLM	6.34	6.63
BPPCT036	FH_2022，VC_2022，TA_2023	GLM	5.64 ~ 8.76	7.82 ~ 11.52
BPPCT037	VC_2023	GLM	6.52	9.23
BPPCT040	TSS_2022	GLM	6.32	7.14
CPDCT016	FS_2022，TS_2022	GLM	7.11 ~ 7.96	8.79 ~ 8.82
CPDCT028	FS_2022，EA_2022，FH_2022，TSS_2022	GLM	7.54 ~ 8.51	8.55 ~ 10.90
CPPCT025	FS_2022	GLM	6.37	6.91
CPPCT1	EA_2022，FH_2022，RS_2023	GLM	5.35 ~ 9.19	7.03 ~ 11.12
CPPCT13	FW_2022，EA_2022，FH_2022，FH_2023	GLM，MLM	4.47 ~ 8.11	7.59 ~ 9.93
CPPCT22	FH_22	GLM	9.65	12.85
CPPCT26	FW_2022，EA_2022，FH_2022，RS_2022，VC_2022，VC_2023	GLM，MLM	4.29 ~ 8.46	7.03 ~ 10.18
CPPCT34	FS_2022，FS_2023，FH_2023	GLM，MLM	6.01 ~ 14.42	7.99 ~ 17.22
CPPCT35	EA_2022，TS_2022	GLM，MLM	7.48 ~ 10.39	7.37 ~ 12.77
CPPCT42	VC_2022，VC_2023	GLM	5.96 ~ 6.20	8.29 ~ 8.37
CPSCT004	FH_2022	GLM	6.46	8.24
CPSCT005	TD_2022，TD2023，FH_2022，TSS_2022，TSS_2023	GLM，MLM	5.01 ~ 10.02	7.06 ~ 11.84
CPSCT006	FH_2022	GLM	10.66	14.39
CPSCT012	LD_2022，LD_2023，FS_2023，FW_2022，TSS_2022，TSS_2023	GLM，MLM	5.24 ~ 8.50	6.03 ~ 12.37
CPSCT018	FS_2022，FS_2023，RS_2022，RS_2023	GLM，MLM	5.39 ~ 9.06	7.23 ~ 12.48
CPSCT021	TS_2022	GLM	6.22	7.63
CPSCT022	FS_2023，FH_2023，TS_2022，VC_2022	GLM，MLM	5.64 ~ 9.02	6.93 ~ 9.46
CPSCT023	VC_2023	GLM	5.35	7.45
CPSCT024	FS_2022，FS_2023，EA_2022，TS_2022	GLM，MLM	5.79 ~ 13.08	6.82 ~ 18.85
CPSCT032	TS_2022	MLM	8.63	8.47
CPSCT033	TSS_2023	GLM	5.65	7.00
EPDCU5100	FW_2022	GLM，MLM	5.13 ~ 5.51	6.97 ~ 8.54
GS1	LD_2023，FS_2022，FS_2023，FH_2022，TSS_2022，TSS_2023	GLM，MLM	4.01 ~ 8.49	4.94 ~ 10.20
PceGA025	LD_2023，FS_2022，FH_2022，TSS_2023	GLM，MLM	4.61 ~ 9.03	7.22 ~ 11.92
PchgmS2	LD_2022，FS_2022，FS_2023，TS_2022，TSS_2022	GLM，MLM	5.16 ~ 10.11	5.02 ~ 11.96
PchgmS4	LD_2022，FW_2022，FW_2023，TSS_2023	GLM，MLM	4.67 ~ 6.87	7.95 ~ 9.59
PchgmS6	EA_2022，FH_2022	GLM	5.76 ~ 9.54	7.27 ~ 11.59
PGS1.21	FW_2023，FH_2023	GLM，MLM	4.01 ~ 6.79	5.73 ~ 9.12
PGS1.24	TS_2022，TS_2023	GLM，MLM	4.47 ~ 8.02	5.41 ~ 9.53
PS08E08	FS_2023，FH_2023	GLM	6.08 ~ 11.36	12.36 ~ 16.33
PS12A02	LD_2023，FH_2022，FH_2023，VC_2022	GLM，MLM	4.56 ~ 11.09	7.18 ~ 15.07
UDP96-003	FH_2022	GLM	6.65	7.04
UDP96-008	TSS_2023	GLM，MLM	8.86 ~ 9.02	9.24 ~ 11.45
UDP98-024	FH_2022	GLM	7.58	9.80
UDP98-025	FH_2022，FH_2023，VC_2022，VC_2023	GLM，MLM	6.06 ~ 14.38
UDP98-409	TSS_2022，TSS_2023	GLM，MLM	5.79 ~ 9.96	6.86 ~ 11.75
UDP98-412	TS_2022	GLM	6.78	8.36

引物 Primer	BPPCT001	BPPCT002	BPPCT004	BPPCT007	BPPCT008	BPPCT010
指纹代码 Fingerprint code	111100	0000	10011	00111111111	0110	111
引物Primer	BPPCT012	BPPCT017	BPPCT024	BPPCT025	BPPCT026	BPPCT029
指纹代码 Fingerprint code	0011	100	111111	00001	10	000000
引物Primer	BPPCT030	BPPCT033	BPPCT034	BPPCT036	BPPCT037	CPPCT1
指纹代码 Fingerprint code	011000	11110111111	111	10101	000	11010000
引物Primer	CPPCT13	CPPCT042	CPSCT005	CPSCT006	CPSCT012	CPSCT018
指纹代码 Fingerprint code	000111	1110	0111111	111000	11101	0111
引物Primer	CPSCT021	CPSCT034	CPSCT039
指纹代码 Fingerprint code	11110	0100	01111

[1]	QIN Zilu, XU Zhengkang, DAI Xiaogang, CHEN Yingnan. Genetic Diversity Analysis and Core Collection Construction of Magnolia biondii Germplasm [J]. Acta Horticulturae Sinica, 2024, 51(8): 1823-1832.
[2]	QIN Yu, GUO Rongkun, NONG Huilan, WANG Yan, CUI Kai, DONG Ningguang. Using the Fluorescent Labeled SSR Markers to Establish Molecular Identity of Hawthorn [J]. Acta Horticulturae Sinica, 2024, 51(6): 1227-1240.
[3]	YANG Xi, RAO Dehua, LIU Hong, YANG Zhe, SU Zhenzhu, JIANG Yuan, YIN Jiwei, and XU Zhenjiang, . Association Analysis of Cattleya DUS Test Characteristics and SSR Markers [J]. Acta Horticulturae Sinica, 2024, 51(4): 787-803.
[4]	ZHANG Zhenghai, WANG Yongfu, WU Huamao, YU Hailong, CAO Yacong, FENG Xigang, WANG Lihao. Advances in Genetic Mapping and Candidate Gene Analysis of Important Traits in Pepper（Capsicum spp.） [J]. Acta Horticulturae Sinica, 2024, 51(3): 669-696.
[5]	XU Qin, WANG Jiaying, ZHANG Mannan, XIAO Zhihao, ZHENG Hankai, LU Yong'en, WANG Taotao, ZHANG Yuyang, ZHANG Junhong, YE Zhibiao, YE Jie. Identification of Genetic Loci and Molecular Marker Development of Salt Tolerance in Tomato Seedlings [J]. Acta Horticulturae Sinica, 2024, 51(2): 239-252.
[6]	CHEN Fangce, HU Pingzheng, XIE Weiwei, WANG Zhengpeng, ZHONG Chuangnan, LI Haiyan, HE Yehua, PENG Ze, WAN Baoxiong, LIU Chaoyang. Development and Application of Prunus salicina InDel Markers Based on Genome Re-sequencing [J]. Acta Horticulturae Sinica, 2024, 51(11): 2510-2522.
[7]	YAO Yuan, DENG Lijun, HU Juan, TANG Xiaoyu, WANG Tie, LI Hang, SUN Guochao, XIONG Bo, LIAO Ling, and WANG Zhihui, . Whole Genome Resequencing Analysis of‘Cuihongli’Plum and Its Early-Ripening Bud Sport Mutation [J]. Acta Horticulturae Sinica, 2024, 51(10): 2255-2266.
[8]	LI Chunniu, SU Qun, LI Xianmin, HUANG Zhanwen, SUN Mingyan, LU Jiashi, WANG Hongyan, and BU Zhaoyang. SSR Molecular Markers Development and Parentage Relationship Identification Based on Whole Genomic Sequences of Jasminum sambac [J]. Acta Horticulturae Sinica, 2024, 51(10): 2343-2357.
[9]	YANG Xinyu , GOU Yunxia , WU Wanying , HE Runlin , DING Qiong , and WANG Dongliang, . A New Plum Blossom‘Wanjianglve’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 173-174.
[10]	NIE Xinghua, ZHANG Yu, LIU Song, YANG Jiabin, HAO Yaqiong, LIU Yang, QIN Ling, XING Yu. Study on Genetic Characteristics and Taxonomic Status of Wild Chinese Chestnut Based on Genome Re-sequencing [J]. Acta Horticulturae Sinica, 2023, 50(8): 1622-1636.
[11]	MEI Yulin, XU Jinjian, JIANG Mengyue, YU Junhai, ZONG Yu, CHEN Wenrong, LIAO Fanglei, GUO Weidong. Identification of F₁ Hybrids and Analysis of Fruit Shape Difference Between Fingered Citron and Citron [J]. Acta Horticulturae Sinica, 2023, 50(7): 1402-1418.
[12]	KAN Zhiyong, ZHANG Dehui, LI Zhongxing, YU Sisi, QIAN Qian, FAN Tianle, LI Xuewei, MA Fengwang, GUAN Qingmei. Evaluation of Cold Tolerance of 90 Apple Cultivars and Genome Wide Association Analysis [J]. Acta Horticulturae Sinica, 2023, 50(5): 921-932.
[13]	SUN Zeshuo, JIANG Dongyue, LIU Xinhong, SHEN Xin, LI Yingang, QU Yufei, LI Yonghua. Cluster Analysis and Construction of DNA Fingerprinting of 42 Oriental Cultivars of Flowering Cherry Based on SSR Markers [J]. Acta Horticulturae Sinica, 2023, 50(3): 657-668.
[14]	WANG Rui, HONG Wenjuan, LUO Hua, ZHAO Lina, CHEN Ying, WANG Jun. Construction of SSR Fingerprints of Pomegranate Cultivars and Male Parent Identification of Hybrids [J]. Acta Horticulturae Sinica, 2023, 50(2): 265-278.
[15]	XIE Qian, ZHANG Shiyan, JIANG Lai, DING Mingyue, LIU Lingling, WU Rujian, CHEN Qingxi. Analysis of Canarium album Transcriptome SSR Information and Molecular Marker Development and Application [J]. Acta Horticulturae Sinica, 2023, 50(11): 2350-2364.