Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (3): 657-668.doi: 10.16420/j.issn.0513-353x.2021-1057

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Cluster Analysis and Construction of DNA Fingerprinting of 42 Oriental Cultivars of Flowering Cherry Based on SSR Markers

SUN Zeshuo1,2, JIANG Dongyue2, LIU Xinhong2, SHEN Xin2, LI Yingang2, QU Yufei3, LI Yonghua1,*()   

  1. 1College of Landscape Architecture and Art,Henan Agricultural University,Zhengzhou 450002,China
    2Zhejiang Academy of Forestry,Hangzhou 310023,China
    3College of Forestry and Biotechnology,Zhejiang A & F University,Hangzhou 310023,China
  • Received:2022-09-19 Revised:2022-12-01 Online:2023-03-25 Published:2023-04-03
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In this study,14 pairs of SSR primers were used to cluster 42 oriental cultivars of flowering cherry based on UPGMA method,and to construct DNA fingerprints by combining primers and genotypes. The results showed that a total of 187 alleles were detected from 14 pairs of SSR primers,with an average of 13.36 alleles per pair of primers and an average of 6.10 effective alleles. The average observed heterozygosity was 0.31,the average expected heterozygosity was 0.80,the average Shannon’s information index was 2.01,and the average polymorphism information content was 0.77. In total,there were 219 genotypes detected,with an average of 15.64 genotypes and an average identified rate of 22.11%. When the Dice genetic similarity coefficient was about 0.18,they could be divided into three groups. Group A mainly consisted of cultivars of Prunus serrulata series,P. incisa series and P. spachiana series,group B consisted of cultivars of P. campanulata series,and group C only had P. dielsiana. When the Dice genetic similarity coefficient was about 0.37,group A could be divided into six groups in addition to P. spachiana‘Plena Rosea’. Meanwhile,the DNA fingerprints of 42 oriental cultivars of flowering cherry were constructed by primers and genotypes. This study can provide a reliable basis for molecular identification of oriental cultivars of flowering cherry in the future.

Key words: flowering cherry, SSR, cultivar, cluster analysis, DNA fingerprint

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