Comparative Analysis of Genetic Background of‘Dongshi Zaoyou’，‘Shatianyou’and‘Shuijingyou’Pummelos

doi:10.16420/j.issn.0513-353x.2022-0981

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (11): 2337-2349.doi: 10.16420/j.issn.0513-353x.2022-0981

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Comparative Analysis of Genetic Background of‘Dongshi Zaoyou’，‘Shatianyou’and‘Shuijingyou’Pummelos

LIU Chenglang¹^,^*, FENG Di¹^,^*, CAO Zonghong¹, YAN Suyun³, ZHANG Yongqing¹, XU Xiangzeng², GAO Shide², YE Junli¹, CHAI Lijun¹, XIE Zongzhou¹^,^**(), DENG Xiuxin¹^,^**()

¹ Key Laboratory of Horticultural Plant Biology（Ministry of Education），College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China
² Yunnan Institute of Tropical Crops，Jinghong，Yunnan 666100，China
³ Institute of Tropcal and Subtropical Cash Crops，Yunnan Academy of Agricultural Scienles，Baoshan，Yunnan 678000，China

Received:2023-05-31 Revised:2023-08-28 Online:2023-11-25 Published:2023-11-28
Contact: XIE Zongzhou, DENG Xiuxin

Abstract

Abstract:

‘Dongshi Zaoyou’，a new pummelo cultivar selected from pummelo seedlings，is mainly cultivated in the tropical areas of Yunnan Province. It has good characteristics such as early-maturing，high-yielding，seedless and etc. However，the genetic origin of‘Dongshi Zaoyou’pummelo has not yet been clarified，which was unfavorable to its protection and utilization. In this study，the cross-compatibility test and molecular markers including self-incompatibility S-genotype，SNP and InDels were used to explore the genetic background differences between‘Dongshi Zaoyou’pummelo and its two similar cultivars ‘Shatianyou’，‘Shuijingyou’. The pollination experiments revealed the cross-incompatibility between‘Dongshi Zaoyou’pummelo and‘Shuijingyou’pummelo while the cross-compatibility between‘Dongshi Zaoyou’pummelo and‘Shatianyou’pummelo. Accordingly，S-genotype of‘Dongshi Zaoyou’pummelo was judged to be S₅S₆，the same as that of‘Shuijingyou’pummelo（S₅S₆），while different from that of ‘Shatianyou’pummelo（S₁S₂）. Based on the genome resequencing data，the genome-wide SNP density distribution of three pummelo cultivars were then plotted and compared. The results showed that‘Dongshi Zaoyou’pummelo and‘Shuijingyou’pummelo had highly similar SNP density and clustered into a group，but they both were significantly different from‘Shatianyou’pummelo. In addition，two InDel polymorphic markers could clearly distinguish‘Dongshi Zaoyou’pummelo from‘Shatianyou’pummelo. Collectively，it could be concluded that‘Dongshi Zaoyou’pummelo showed a closer relationship to‘Shuijingyou’pummelo than‘Shatianyou’pummelo.

Key words: ‘Dongshi Zaoyou’pummelo, S-genotype, InDel marker, SNP

LIU Chenglang, FENG Di, CAO Zonghong, YAN Suyun, ZHANG Yongqing, XU Xiangzeng, GAO Shide, YE Junli, CHAI Lijun, XIE Zongzhou, DENG Xiuxin. Comparative Analysis of Genetic Background of‘Dongshi Zaoyou’，‘Shatianyou’and‘Shuijingyou’Pummelos[J]. Acta Horticulturae Sinica, 2023, 50(11): 2337-2349.

Figures/Tables 11

References 29

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引物名称 Primer name	引物序列（5′-3′） Sequence	退火温度/℃ Annealing temperature
CH1	F：ATGAATGCTTGTGCAAGGACAGCTAAATTC；R：TTAAAGATTTTCCATCTGGGTAAAAGCAT	56
CH2	F：GAGGGCAGCAGTTTGAATCC；R：TGGGACCAACTTACATCAATGC	55
CH3	F：CGTTCGAGTCGTCTTTGTTGG；R：ATACACTGTAAGAGAGATCGTAACA	56
CH4	F：ATGAATGCTTGTGCAAGGACAGCTAAATTCCAG；R：TTAAAGATTTTCCATCTGGGTAAAAGCATCTTC	55
CH5	F：CGCGGAGTCAGCAACAAAAA；R：ACACTGTAAGAGAGATCGTAACAT	55
CH6	F：AGCAACCCGAAACACAAACAC；R：ACACTGTAAGAGAGATCGTAACATT	55
CH7	F：GGATTTGGCTTTTCATCTTCTCA；R：TGCTAATGAGAGATAGTGGGGAG	56

引物名称 Primer name	引物序列（5′-3′） Sequence	退火温度/℃ Annealing temperature
CH1	F：ATGAATGCTTGTGCAAGGACAGCTAAATTC；R：TTAAAGATTTTCCATCTGGGTAAAAGCAT	56
CH2	F：GAGGGCAGCAGTTTGAATCC；R：TGGGACCAACTTACATCAATGC	55
CH3	F：CGTTCGAGTCGTCTTTGTTGG；R：ATACACTGTAAGAGAGATCGTAACA	56
CH4	F：ATGAATGCTTGTGCAAGGACAGCTAAATTCCAG；R：TTAAAGATTTTCCATCTGGGTAAAAGCATCTTC	55
CH5	F：CGCGGAGTCAGCAACAAAAA；R：ACACTGTAAGAGAGATCGTAACAT	55
CH6	F：AGCAACCCGAAACACAAACAC；R：ACACTGTAAGAGAGATCGTAACATT	55
CH7	F：GGATTTGGCTTTTCATCTTCTCA；R：TGCTAATGAGAGATAGTGGGGAG	56

引物名称 Primer name	引物序列（5′-3′） Sequence	退火温度/℃ Annealing temperature
S₁	F：ATGAACATTACTTTCTTCCTCTA；R：TTAAGGCGGGGGAAAGGTA	55
S₂	F：ATGATATCGACAAAGACGAAAA；R：CTACTCATCGGTCGGCTCG	55
S₅	F：ATGAAGGTGGCATCCATCAAC；R：CTACCACCGTGGTGGGAAAATAATATCC	56
S₆	F：ATGGGGACTAATTTCCTCATTATC；R：CTATATTTTAACGTATCGCGGC	55

引物名称 Primer name	引物序列（5′-3′） Sequence	退火温度/℃ Annealing temperature
S₁	F：ATGAACATTACTTTCTTCCTCTA；R：TTAAGGCGGGGGAAAGGTA	55
S₂	F：ATGATATCGACAAAGACGAAAA；R：CTACTCATCGGTCGGCTCG	55
S₅	F：ATGAAGGTGGCATCCATCAAC；R：CTACCACCGTGGTGGGAAAATAATATCC	56
S₆	F：ATGGGGACTAATTTCCTCATTATC；R：CTATATTTTAACGTATCGCGGC	55

试验材料 Plant material	单果质量/g Fruit weight	纵径/mm Vertical diameter	横径/mm Transverse diameter	果形指数 Fruit shape index
‘东试早柚’Dongshi Zaoyou	1 062.17 ± 25.34 bc	151.18 ± 9.01 b	143.66 ± 2.55 b	1.05 ± 0.06 ab
‘沙田柚’Shatianyou	1 543.60 ± 165.96 a	192.06 ± 8.96 a	160.61 ± 10.74 a	1.20 ± 0.05 a
‘水晶柚1’Shuijingyou 1	1 207.26 ± 63.46 bc	152.99 ± 1.35 b	144.49 ± 6.25 b	1.06 ± 0.05 ab
‘水晶柚2’Shuijingyou 2	1 048.00 ± 123.57 c	155.99 ± 14.24 b	149.95 ± 4.19 ab	1.04 ± 0.09 b
‘水晶柚3’Shuijingyou 3	1 349.62 ± 204.80 ab	187.94 ± 10.11 a	158.14 ± 7.81 ab	1.19 ± 0.06 a
试验材料 Plant material	可溶性固形物/% Total soluble solid	可滴定酸/% Titratable acid	固酸比 Solidity-acid ratio	维生素C/（mg · kg^-1） Vitamin C
‘东试早柚’Dongshi Zaoyou	10.93 ± 0.19 a	0.16 ± 0.02 b	71.36 ± 9.49 a	788.8 ± 1.32 a
‘沙田柚’Shatianyou	8.55 ± 0.70 b	0.41 ± 0.18 a	27.20 ± 15.82 b	646.0 ± 8.47 b
‘水晶柚1’Shuijingyou 1	9.44 ± 0.40 b	0.32 ± 0.07 ab	30.32 ± 5.44 b	570.7 ± 4.57 b
‘水晶柚2’Shuijingyou 2	8.95 ± 0.24 b	0.18 ± 0.03 ab	52.51 ± 10.48 b	568.2 ± 2.71 b
‘水晶柚3’Shuijingyou 3	8.88 ± 0.62 b	0.25 ± 0.05 ab	37.00 ± 5.35 b	539.6 ± 6.22 b

Comparative Analysis of Genetic Background of‘Dongshi Zaoyou’，‘Shatianyou’and‘Shuijingyou’Pummelos

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