Abstract:

Based on the nucleotide sequences of Rfo related to the Ogura-CMS fertility restorer gene
in radish，the SNP（Single nucleotide polymorphism）marker named Rfo-SNP1 was developed by use of
KASP（kompetitive allele specific PCR）technology. The genotypes of fertility restorer gene in 24 radish
varieties including 304 plants were identified using the Rfo-SNP1 marker. The results showed that 289
plants were screened out accounting for 95.07% of the whole population. And 110 plants had the recessive
homozygous genotype（rfrf）taking up 36.18%. The dominant homozygous genotype（RfRf）and the
heterozygous genotype（Rfrf）appeared respectively in 156 plants and 23 plants. In 23 fertile varieties，12
varieties had the rfrf genotype，which implied that the 12 varieties might be the candidates of maintainer
lines for breeding sterile lines. The accuracy of Rfo-SNP1 markers was further verified by PCR-RFLP and backcross populations. Six male sterile lines and maintainer lines of radish have been developed through
this procedure.

Key words: radish, Ogura-CMS, fertility restorer gene Rfo, SNP marker, assisted selection