Abstract:

To create Chinese cabbage–cabbage translocation lines，isolated microspore culture was carried out using the progeny derived from crossing between Chinese cabbage–cabbage alien addition line AC9 and Chinese cabbage‘85-1’. Two double haploid translocation lines‘AT9-1’and‘AT9-2’with different fragments of cabbage chromosome 9 were screened out combining chromosome specific InDel markers and meiosis observation. The exogenetic fragment size of the translocation line‘AT9-2’was further identified by additional InDel markers，with the value of 1.03 Mb. Using 177 InDel markers uniformly distributed on ten linkage groups of Chinese cabbage，the cabbage chromosome fragment of translocation lines‘AT9-1’and‘AT9-2’were selfed，backcrossed with the parent‘85-1’，and hybridized with Chinese cabbage inbred lines‘14-28’and‘14-36’，and their offspring individuals were identified by the specific InDel markers from linkage group C06 of cabbage. The results showed that the introduced chromosome fragments from cabbage in the two translocation lines was instability and very low in genetics，with ratio of keeping entire exogenous fragment in their selfing progenies，backcross progenies and two hybridization progenies of 8.9%，3.1%，2.8% for‘AT9-1’and 6.7%，1.6%，2.6% for‘AT9-2’，respectively.

Key words: Chinese cabbage, cabbage, translocation line, InDel marker, genetic stability