Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (11): 2376-2386.doi: 10.16420/j.issn.0513-353x.2022-0854
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
WANG Min, YANG Songguang, LIU Wei, HE Xiaoming, SHI Shaoqi, LIU Wenrui, CHEN Lin, JIANG Biao, PENG Qingwu*()
Received:
2023-03-21
Revised:
2023-09-18
Online:
2023-11-25
Published:
2023-11-28
Contact:
PENG Qingwu
WANG Min, YANG Songguang, LIU Wei, HE Xiaoming, SHI Shaoqi, LIU Wenrui, CHEN Lin, JIANG Biao, PENG Qingwu. Response to Low Temperature Stress and Screening and Validation of Interaction Protein of CqCHP1 in Chieh-qua[J]. Acta Horticulturae Sinica, 2023, 50(11): 2376-2386.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2022-0854
用途 | 名称 | 引物序列(5′-3′) |
---|---|---|
Use | Name | Primer sequence |
基因克隆Gene cloning | CqCHP1-F | ATGGCGCTGGTACCCGGAAA |
CqCHP1-R | CTAAGCACCAACAGAACCGA | |
实时荧光定量PCR | CqCHP1-QF | ACATACTGCTCCGATTCCCC |
Quantitative real-time PCR | CqCHP1-QR | TTGGGGTGAATATTGACTCC |
亚细胞定位 Sublocation | 1305-GFP-CqCHP1-F | CGAGCTGTACAGATCTATGGCGCTGGTACCCGGAAA |
1305-GFP-CqCHP1-R | GGCCGCTTTAAGATCTCTACTAAGCACCAACAGAACCGA | |
酵母双杂 Yeast two-hybrid | BD-CqCHP1-F | GAATTCCCGGGGATCCGTATGGCGCTGGTACCCGGAAA |
BD-CqCHP1-R | GCAGGTCGACGGATCCCTAAGCACCAACAGAACCGA | |
AD-bHLH92-F | CATCGATACGGGATCCATGGATGATAGCTTTTCTGT | |
AD-bHLH92-R | CGAGCTCGATGGATCCTTAGCTTTCAAATTCTGGAC | |
双分子荧光互补 | CqCHP1-BIFC-N-F | CCCTAGGAACTAGTATGGCGCTGGTACCCGGAAA |
Bimolecular fluorescence | CqCHP1-BIFC-N-R | GATCCTCCACTAGTCTAAGCACCAACAGAACCGA |
complementation | bHLH92-BIFC-C-F | TACGTAGTCACGTGATGGATGATAGCTTTTCTGT |
bHLH92-BIFC-C-R | CCGGACGTCACGTGTTAGCTTTCAAATTCTGGAC | |
荧光素酶互补 | bHLH92-nLUC-F | ACGGGGGACGAGCTCGGTACCATGGATGATAGCTTTTCTGTG |
Luciferase complementation | bHLH92-nLUC-R | CGCGTACGAGATCTGGTCGACGCTTTCAAATTCTGGACCCACA |
imaging assay | CqCHP1-cLUC-F | TACGCGTCCCGGGGCGGTACCATGGCGCTGGTACCCGGAAA |
CqCHP1-cLUC-R | ACGAAAGCTCTGCAGGTCGACTCAAGCACCAACAGAACCGAAAAT |
Table 1 Primer sequences used in this study
用途 | 名称 | 引物序列(5′-3′) |
---|---|---|
Use | Name | Primer sequence |
基因克隆Gene cloning | CqCHP1-F | ATGGCGCTGGTACCCGGAAA |
CqCHP1-R | CTAAGCACCAACAGAACCGA | |
实时荧光定量PCR | CqCHP1-QF | ACATACTGCTCCGATTCCCC |
Quantitative real-time PCR | CqCHP1-QR | TTGGGGTGAATATTGACTCC |
亚细胞定位 Sublocation | 1305-GFP-CqCHP1-F | CGAGCTGTACAGATCTATGGCGCTGGTACCCGGAAA |
1305-GFP-CqCHP1-R | GGCCGCTTTAAGATCTCTACTAAGCACCAACAGAACCGA | |
酵母双杂 Yeast two-hybrid | BD-CqCHP1-F | GAATTCCCGGGGATCCGTATGGCGCTGGTACCCGGAAA |
BD-CqCHP1-R | GCAGGTCGACGGATCCCTAAGCACCAACAGAACCGA | |
AD-bHLH92-F | CATCGATACGGGATCCATGGATGATAGCTTTTCTGT | |
AD-bHLH92-R | CGAGCTCGATGGATCCTTAGCTTTCAAATTCTGGAC | |
双分子荧光互补 | CqCHP1-BIFC-N-F | CCCTAGGAACTAGTATGGCGCTGGTACCCGGAAA |
Bimolecular fluorescence | CqCHP1-BIFC-N-R | GATCCTCCACTAGTCTAAGCACCAACAGAACCGA |
complementation | bHLH92-BIFC-C-F | TACGTAGTCACGTGATGGATGATAGCTTTTCTGT |
bHLH92-BIFC-C-R | CCGGACGTCACGTGTTAGCTTTCAAATTCTGGAC | |
荧光素酶互补 | bHLH92-nLUC-F | ACGGGGGACGAGCTCGGTACCATGGATGATAGCTTTTCTGTG |
Luciferase complementation | bHLH92-nLUC-R | CGCGTACGAGATCTGGTCGACGCTTTCAAATTCTGGACCCACA |
imaging assay | CqCHP1-cLUC-F | TACGCGTCCCGGGGCGGTACCATGGCGCTGGTACCCGGAAA |
CqCHP1-cLUC-R | ACGAAAGCTCTGCAGGTCGACTCAAGCACCAACAGAACCGAAAAT |
Fig. 2 Analysis of phylogenetic of CqCHP1 Os:Oryza sativa L.;AT:Arabidopsis thaliana(L.)Heynh;Csa:Cucumis sativus;Solyc:Solanum lycopersicum;A4A:Nicotiana attenuata.
Fig. 6 Yeast self-activation and interaction identification of CqCHP1 in yeast -LW:-Leu/-Trp;-ALWH:-Ade/-His/-Leu/-Trp;-MEL1:-Ade/-His/-Leu/-Trp/X-α-gal.
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