Analysis of Freezing Tolerances and Physiological Differences of Three Pomegranate Cultivars During the Overwintering

doi:10.16420/j.issn.0513-353x.2022-0551

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (7): 1563-1573.doi: 10.16420/j.issn.0513-353x.2022-0551

• Cultivation Physiology & Biochemistry • Previous Articles Next Articles

Analysis of Freezing Tolerances and Physiological Differences of Three Pomegranate Cultivars During the Overwintering

TANG Haixia¹, YANG Xuemei¹, FENG Lijuan¹, ZHU Feng², ZHOU Jilei³, YIN Yanlei¹^,^*()

¹ Shandong Institute of Pomology，Tai'an，Shandong 271000，China
² Laiwu District Bureau of Agriculture and Rural Affairs，Jinan 271100，China
³ Shandong Forestry Protection and Development Service Center，Jinan 250012，China

Received:2023-02-23 Revised:2023-04-21 Online:2023-07-25 Published:2023-07-26
Contact: YIN Yanlei

Abstract

Abstract:

To study the change of freezing tolerance and its physiological basis of annual shoots of ‘Taishanhong’‘Luqing 1’and‘Tunisian Soft Seed’pomegranates during overwintering，the cold hardiness was estimated by the semi-lethal temperature（LT₅₀），which was assessed by electrolyte leakage rate with Logistic quation，the content of relative water，soluble protein，soluble sugar，malondialdehyde（MDA），activities of peroxidase（POD）and superoxidase（SOD），field overwintering rate，botanical characteristics of shoots，and the recovery ability of branches after freezing stress were measured. The results showed that the significant differences in cold resistance among three pomegranate cultivars. The overwintering rate of‘Luqing 1’was much higher than those of‘Taishanhong’and‘Tunisian Soft Seed’，and‘Tunisian Soft Seed’was the worst. Compared with‘Tunisia Soft Seed’，the shoots of‘Luqing 1’ were thick，hard，with short internodes，the spiness were also strong and hard. Sealing layers of lenticle were rich and tight. The changes of physiological indexes of‘Luqing 1’were relatively small after low temperature treatment. MDA content was low，soluble proteins content and POD activity were high. The osmotic pressure and redox balance of‘Luqing 1’could be maintained well，so its cold resistance much better than that of‘Tunisan Soft Seed’.

Key words: pomegranate, freezing tolerance, branch, semi-lethal temperature, morphological characteristic, anatomical structure

TANG Haixia, YANG Xuemei, FENG Lijuan, ZHU Feng, ZHOU Jilei, YIN Yanlei. Analysis of Freezing Tolerances and Physiological Differences of Three Pomegranate Cultivars During the Overwintering[J]. Acta Horticulturae Sinica, 2023, 50(7): 1563-1573.

Figures/Tables 9

References 32

[1]	Bai Lu, Zhang Zhiguo, Zhang Shijie, Huang Dongmei, Qin Qiaoping. 2021. Isolation of three types of invertase genes from Hemerocallis fulva and their responses to low temperature and osmotic stress. Acta Horticulturae Sinica, 48 (2):300-312. (in Chinese)
	白露, 张志国, 张世杰, 黄东梅, 秦巧平. 2021. 萱草3 种蔗糖转化酶基因的分离及对低温和渗透胁迫响应的分析. 园艺学报, 48 (2):300-312. doi: 10.16420/j.issn.0513-353x.2020-0346
[2]	Guo Yan, Zhang Shu-hang, Li Ying, Zhang Xin-fang, Han Bin, Wang Guang-peng, Yang Yang. 2019. Comprehensive evaluation on the cold resistance of several main Chinese chestnut cultivars. Journal of China Agricultural University, 24 (4):52-63. (in Chinese)
	郭燕, 张树航, 李颖, 张馨方, 韩斌, 王广鹏, 杨阳. 2019. 我国几个板栗品种抗寒性综合评价. 中国农业大学学报, 24 (4):52-63.
[3]	Jiao Qi-qing, Feng Li-juan, Yin Yan-lei, Cui Hong-tao. 2019. Research progress on evaluation of freezing injury and cold resistance of pomegranate. Plant Physiology Journal, 55 (4):425-432. (in Chinese)
	焦其庆, 冯立娟, 尹燕雷, 崔洪涛. 2019. 石榴冻害及抗寒评价研究进展. 植物生理学报, 55 (4):425-432.
[4]	Ju Zhi-xin. 2011. Study on the ecological adaptation and cold resistance of tree peony in Northeast China[Ph. D. Dissertation]. Beijing: Beijing Forestry University. (in Chinese)
	鞠志新. 2011. 东北地区牡丹生态适应性及抗寒性研究[博士论文]. 北京: 北京林业大学.
[5]	Li Hong-lian, Wang Qiang, Yan Xing-kai, Lu Ming-yan, Zhang Mao-jun. 2015. Analysis of correlation between lenticels and cold hardiness of pear. Journal of Jilin Agricultural Science, 40 (6):94-97. (in Chinese)
	李红莲, 王强, 闫兴凯, 卢明艳, 张茂君. 2015. 梨枝条皮孔与抗寒能力相关性的研究. 吉林农业科学, 40 (6):94-97.
[6]	Li Jian-xiong. 2018. Investigation and analysis of pomegranate frost damage in Fangshan District of Beijing. Xiandai Horticulture,(8):7-8. (in Chinese)
	李剑雄. 2018. 北京房山地区石榴冻害调查分析. 现代园艺,(8):7-8.
[7]	Li Ming-wan. 2010. Sudden drop of temperature on pomegranate caused by freezing injury and protection. Bull Agric Sci Technol,(11):167-169. (in Chinese)
	李明婉. 2010. 骤然降温对石榴造成的冻害及防护. 农业科技通讯,(11):167-169.
[8]	Liu Bei-bei, Chen Li-na, Niu Juan, Li Hao-xian, Zhang Jie, Cao Shangyin. 2018. Selection of methods for evaluation on cold tolerance of six pomegranate varieties. Journal of Fruit Science, 35 (1):66-73. (in Chinese)
	刘贝贝, 陈利娜, 牛娟, 李好先, 张杰, 曹尚银. 2018. 6个石榴品种抗寒性评价及方法筛选. 果树学报, 35 (1):66-73.
[9]	Liu Qiang, Yang Shu-hua, Jia Rui-dong, Zhao Xin, Ge Hong. 2017. Analysis of freezing tolerances and its physiological differences of two Rosa species during the overwintering. Acta Horticulturae Sinica, 44 (7):1344-1354. (in Chinese) doi: 10.16420/j.issn.0513-353x.2016-0880
	刘强, 杨树华, 贾瑞冬, 赵鑫, 葛红. 2017. 弯刺蔷薇与大花白木香越冬抗寒性及其生理差异分析. 园艺学报, 44 (7):1344-1354. doi: 10.16420/j.issn.0513-353x.2016-0880
[10]	Liu Xia, Hou Le-feng, Hao Zhao-xiang, Bi Run-xia, Li Zhao-hui, Ma Min, Zhang Li-hua. 2015. Evaluation of cold resistances of 12 Punica granatum cultivars by conductivity method. Nonwood Forest Research,(3):150-153. (in Chinese)
	刘霞, 侯乐峰, 郝兆祥, 毕润霞, 李昭慧, 马敏, 张立华. 2015. 以电导法评价12个石榴品种的抗寒性. 经济林研究,(3):150-153.
[11]	Liu Ying, Zeng Qi-Yao, Zeng Bing-shan, Huang Lie-jian, He Wei-zhong, He Bi-hong. 2020. Physiological analysis on cold resistance of Albizia falcataria clones. Journal of Central South University of Forestry Technology, 40 (5):7-12. (in Chinese)
	刘英, 曾琪瑶, 曾炳山, 黄烈键, 何卫忠, 何碧红. 2020. 南洋楹无性系抗寒性研究. 中南林业科技大学学报, 40 (5):7-12.
[12]	Luo Hua, Hao Zhao-xiang, Chen Ying, Meng Jian, Wang Qing-jun, Zhao Li-na, Hou Lefeng. 2018. Evaluation of cold resistance of new pomegranate varieties(germplasm). China Fruits,(1):51-54. (in Chinese)
	罗华, 郝兆祥, 陈颖, 孟健, 王庆军, 赵丽娜, 侯乐峰. 2018. 石榴新品种(种质)抗寒性评价. 中国果树,(1):51-54.
[13]	Meng Shi-yuan, Lü Gui-yuan, Zhang Ming-zhong, Zhang Zhi-hao, Wei Ye, Wang Hua-tian, Liu Xiu-mei. 2020. Physiological response to cold stress and evaluation of cold resistance of five species of Euonymus Linn. Acta Botanica Boreali-Occidentalia Sinica, 40 (4):624-634. (in Chinese)
	孟诗原, 吕桂云, 张明忠, 张志浩, 韦业, 王华田, 刘秀梅. 2020. 5种卫矛属植物对低温胁迫的生理响应及抗寒性评价. 西北植物学报, 40 (4):624-634.
[14]	Nie Xinmiao, Luan Heng, Feng Gaili, Wang Chao, Li Yan, Wei Min. 2022. Effects of silicon nutrition and grafting rootstocks on chilling tolerance of cucumber seedlings. Acta Horticulturae Sinica, 49 (8):1795-1804. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0379
	聂鑫淼, 栾恒, 冯改利, 王超, 李岩, 魏珉. 2022. 硅营养和嫁接砧木对黄瓜幼苗耐冷性的影响. 园艺学报, 49 (8):1795-1804. doi: 10.16420/j.issn.0513-353x.2021-0379
[15]	Ren Hui, Wang Xiao-mei, Liu Ye-qiang, Su Wei-qiang. 2016. Cold resistance of Averrhoa carambola by application of electrical conductivity and Logistic Equation. Southwest China Journal of Agricultural Sciences, 29 (3):662-667. (in Chinese)
	任惠, 王小媚, 刘业强, 苏伟强. 2016. 应用电导率法和Logistic方程测定杨桃枝条抗寒性的研究. 西南农业学报, 29 (3):662-667.
[16]	Soloklui A A G, Ershadi A, Fallahi E. 2012. Evaluation of cold hardiness in seven Iranian commercial pomegranate(Punica granatum L.)cultivars. HortScience, 47:1821-1825.
[17]	Tang Qi-yi, Feng Ming-guang. 2007. DPS data processing system:experimental design,statistical classification and data minin. Beijing: Science Press. (in Chinese)
	唐启义, 冯明光. 2007. DPS数据处理系统——实验设计、统计分析及数据挖掘. 北京: 科学出版社.
[18]	Tian Jia-cai, Li Jia-liang, Yin Yan-lei, Wang Yue-hua, Li Chun. 2017. Analysis of pomegranate freezing injury in Zaozhuang of Shandong Province in 2015. Deciduous Fruits, 49 (1):57-58. (in Chinese)
	田加才, 李甲梁, 尹燕雷, 王跃华, 李春. 2017. 2015年山东枣庄石榴冻害情况分析. 落叶果树, 49 (1):57-58.
[19]	Wang Hai-ou, Gao Zhan, Aikebaier Mao, Yang Xiao-ping, Zhou Long, Li Shu-de. 2019. Analysis on changes of several physiological indexes of 17 grape rootstock cultivars under natural wintering conditions. Non-wood Forest Research, 37 (4):120-128. (in Chinese)
	王海鸥, 高展, 艾克拜尔•毛拉, 杨小平, 周龙, 李树德. 2019. 自然越冬条件下17个葡萄砧木品种几个生理指标变化分析. 经济林研究, 37 (4):120-128.
[20]	Wang Qian. 2022. Biological investigation and analysis of two new cold resistant wine grape lines[M. D. Dissertation]. Qinhuangdao: Hebei Normal University of Science and Technology. (in Chinese)
	王茜. 2022. 两个抗寒酿酒葡萄新品系的生物学调查及分析[硕士论文]. 秦皇岛. 河北科技师范学院.
[21]	Wang Qing-jun, Bi Run-xia, Ma Min, Meng Jian, Hou Le-feng, Hao Zhao-xiang. 2017. Causes and preventive measures of pomegranate freezing injury in northern China. China Fruits,(2):76-79. (in Chinese)
	王庆军, 毕润霞, 马敏, 孟健, 侯乐峰, 郝兆祥. 2017. 我国北方地区石榴冻害的发生原因及预防措施. 中国果树,(2):76-79.
[22]	Wang Qing-jun, Hao Zhao-xiang, Hou Le-feng, Zhao Li-na, Luo Hua, Meng Jian. 2015. Analysis of cold resistance of three pomegranate cultivars under cold stress. North Horticulture,(2):18-21. (in Chinese)
	王庆军, 郝兆祥, 侯乐峰, 赵丽娜, 罗华, 孟健. 2015. 低温胁迫下三个石榴品种的抗寒性分析. 北方园艺,(2):18-21.
[23]	Wang Qing-jun, Luo Hua, Zhao Li-na, Chen Ying, Wang Yan-qin, Wang Yu-hai, Hao Zhao-xiang. 2018. Evaluation on cold resistance of 24 pomegranate cultivars. Shandong Agricultural Sciences, 50 (1):50-54,59. (in Chinese)
	王庆军, 罗华, 赵丽娜, 陈颖, 王艳芹, 王玉海, 郝兆祥. 2018. 24个石榴品种的抗寒性评价. 山东农业科学, 50 (1):50-54,59.
[24]	Yang Ning-ning, Sun Wan-cang, Liu Zi-gang, Shi Peng-hui, Fang Yan, Wu Jun-yan, Zeng Xiu-cun, Kong De-jing, Lu Mei-hong, Wang Yue. 2014. Morphological characters and physiological mechanisms of cold resistance of winter rape seed in northern China. Scientia Agricultura Sinica, 47 (3):452-461. (in Chinese)
	杨宁宁, 孙万仓, 刘自刚, 史鹏辉, 方彦, 武军艳, 曾秀存, 孔德晶, 鲁美宏, 王月. 2014. 北方冬油菜抗寒性的形态与生理机制. 中国农业科学, 47 (3):452-461. doi: 10.3864/j.issn.0578-1752.2014.03.005
[25]	Yang Sheng, Guo Huang-ping, Hao Guo-wei, Zhang Xiao-wei, Bai Mu-dan, Shi Mei-juan, Cheng Pei-hong, Li Kai. 2014. The study of the cold resistance and physiology index of some pear varieties in Shanxi Province. Journal of Agriculture, 4 (12):72-77. (in Chinese)
	杨盛, 郭黄萍, 郝国伟, 张晓伟, 白牡丹, 石美娟, 程培红, 李凯. 2014. 山西部分梨品种抗寒性测定及生理指标研究. 农学学报, 4 (12):72-77. doi: 10.11923/j.issn.2095-4050.2014-xb0794
[26]	Yang Xue-mei, Yuan Zhao-le, Yin Yan-lei, Feng Li-juan, Zhao Xue-qing. 2014. Comprehensive evaluation on cold resistance of different pomegranate cultivars. Shandong Agricultural Sciences,(2):46-51. (in Chinese)
	杨雪梅, 苑兆和, 尹燕雷, 冯立娟, 招雪晴. 2014. 不同石榴品种抗寒性综合评价. 山东农业科学,(2):46-51.
[27]	Yao Fang, Wang Ning, Cao Shang-yin, Ma Guan-yang, Si Shou-xia, Yao Hai-lei. 2016. Comprehensive evaluation on cold resistance of different pomegranate cultivars. Journal of Forest and Environment, 36 (3):373-379. (in Chinese)
	姚方, 王宁, 曹尚银, 马贯羊, 司守霞, 姚海雷. 2016. 不同软籽品种石榴抗寒性综合评价. 森林与环境学报, 36 (3):373-379.
[28]	Zhang Bo, Liu Li-qiang, Qin Wei, Wurenqimige. 2021. Study on physiological and biochemical mechanism of cold resistance of Malus sieversii. Non-wood Forest Research, 39 (4):60-69. (in Chinese)
	张博, 刘立强, 秦伟, 乌仁其米格. 2021. 新疆野苹果抗寒生理生化机制研究. 经济林研究, 39 (4):60-69.
[29]	Zhang Yan-xia, Luo Hua, Hou Le-feng, Liu Xia, Hao Zhao-xiang, Zhao Li-na, Zhang Li-hua. 2018. Evaluation of cold resistance of five pomegranate varieties. Shandong Agricultural Sciences, 27 (1):50-54. (in Chinese)
	张艳侠, 罗华, 侯乐峰, 刘霞, 郝兆祥, 赵丽娜, 张立华. 2018. 五个石榴品种的抗寒性评价. 山东农业科学, 27 (1):50-54.
[30]	Zhang Yu-lan, Xu Qi-yuan, Wu Rui-xia, Chang Zhan-sheng, Han Gui-qin, Liu Fu-wei. 1991. Relationship between lenticels number,shoot anatomical structure and death of branch in dormant hawthorn. Journal of Inner Mongolia Institute of Agriculture & Animal Husbandry, 12 (3):29-38. (in Chinese)
	张玉兰, 徐奇元, 武瑞霞, 常战胜, 韩桂芹, 刘复伟. 1991. 休眠期山楂枝条皮孔、组织解剖构造与抽条的关系. 内蒙古农牧学院学报, 12 (3):29-38.
[31]	Zhao Li-na, Wang Qing-jun, Chen Ying, Luo hua, Ma Min, Li Ti-song, Zhao Ya-wei. 2018. Evaluation on cold resistance of four ornamental pomegranate. Shandong Forestry Science and Technology,(1):20-28. (in Chinese)
	赵丽娜, 王庆军, 陈颖, 罗华, 马敏, 李体松, 赵亚伟. 2018. 4个观赏石榴品种抗寒性评价. 山东林业科技,(1):20-28.
[32]	Zou Qi. 2003. Experimental guidance of plant physiology. Beijing: China Agriculture Press. (in Chinese)
	邹琦. 2003. 植物生理学实验指导. 北京: 中国农业出版社.

时间/（Y-M） Date	平均低温/℃ Mean maximum temperature	极端低温/℃ Extreme low temperature	2年生突尼斯软籽 2-year-old Tunisian Soft Seed	泰山红Taishanhong		鲁青1号Luqing 1
时间/（Y-M） Date	平均低温/℃ Mean maximum temperature	极端低温/℃ Extreme low temperature	2年生突尼斯软籽 2-year-old Tunisian Soft Seed	2年生 2-year-old	多年生 Many years old	2年生 2-year-old	多年生 Many years old
2015-11—2016-03	-1.8	-18	0	0	53.2	100	100
2016-11—2017-03	-1.5	-9	0	83.6	100	100	100
2017-11—2018-03	-2.8	-12	—	68.4	100	100	100

时间/（Y-M） Date	平均低温/℃ Mean maximum temperature	极端低温/℃ Extreme low temperature	2年生突尼斯软籽 2-year-old Tunisian Soft Seed	泰山红Taishanhong		鲁青1号Luqing 1
时间/（Y-M） Date	平均低温/℃ Mean maximum temperature	极端低温/℃ Extreme low temperature	2年生突尼斯软籽 2-year-old Tunisian Soft Seed	2年生 2-year-old	多年生 Many years old	2年生 2-year-old	多年生 Many years old
2015-11—2016-03	-1.8	-18	0	0	53.2	100	100
2016-11—2017-03	-1.5	-9	0	83.6	100	100	100
2017-11—2018-03	-2.8	-12	—	68.4	100	100	100

品种 Cultivar	处理温度/℃ Processing temperature	拟合曲线 Fitted curve	R²	LT₅₀/℃
突尼斯软籽Tunisian Soft Seed	-15 ~ 5	y = 0.1375x + 0.4696	0.9288	-3.42
泰山红Taishanhong	-15 ~ 5	y = 0.0780x + 0.3767	0.9638	-4.83
鲁青1号Luqing 1	-15 ~ 5	y = 0.0559x + 0.5862	0.9128	-10.49

品种 Cultivar	处理温度/℃ Processing temperature	拟合曲线 Fitted curve	R²	LT₅₀/℃
突尼斯软籽Tunisian Soft Seed	-15 ~ 5	y = 0.1375x + 0.4696	0.9288	-3.42
泰山红Taishanhong	-15 ~ 5	y = 0.0780x + 0.3767	0.9638	-4.83
鲁青1号Luqing 1	-15 ~ 5	y = 0.0559x + 0.5862	0.9128	-10.49

品种 Cultivar	形态 Morphology								相对含水量% RWC		枝粗/cm Width		主枝节间长/mm Internode length of main branch
品种 Cultivar	枝条 Branch			枝刺 Spine			枝刺状短枝Spiny short branch		相对含水量% RWC		枝粗/cm Width		主枝节间长/mm Internode length of main branch
泰山红Taishanhong	较软，皮略带红色 Softer, slightly reddish			较少 Less			末节部分退化 The end part is partially degraded		40.01		1.01		63.28
鲁青1号 Luqing 1	硬，皮亮，偏棕绿色，粗壮 Hard, skin bright brownish, strong			多，粗壮，硬 More, strong, hard					39.10		1.21		38.73
突尼斯软籽 Tunisian Soft Seed	较软，细长，皮偏白色 Softer, slender, bark whiter			多，细长，硬 More, slender, hard					43.99		0.84		37.50
品种 Cultivar	枝刺 Spiny branch		第4节枝刺状枝 The fourth spiny branch					第9节枝刺状枝 The ninth spiny branch			第12节枝刺状枝 The 12th spiny branch
品种 Cultivar	长/mm Length	粗/mm Width	长/mm Length		粗/mm Width	小节数Section number		长/mm Length	粗/mm Width	小节数Section number	长/mm Length	粗/mm Width		小节数Section number
泰山红Taishanhong	7.19	1.24	18.30		1.56	2		66.87	1.61	3	148.59	3.68		6
鲁青1号 Luqing 1	8.39	1.52	41.08		2.11	3		54.07	2.15	4	69.49	2.77		5
突尼斯软籽 Tunisian Soft Seed	9.06	1.06	33.25		1.62	3		56.56	1.94	3	71.72	2.17		4

Analysis of Freezing Tolerances and Physiological Differences of Three Pomegranate Cultivars During the Overwintering

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 9

References 32

Related Articles 15

Recommended Articles

Metrics

Comments

品种Cultivar	横径/μm Transverse diameter	纵径/μm Longitudinal diameter	皮孔面积/μm² Lenticelles area	单位面积皮孔/% Unit area of lenticle	封闭层数 Number of closed layers	木栓形成层 Cork cambium	皮孔状态 Lenticels state
鲁青1号 Luqing 1	256.2 A	321.2 A	59 350.8 A	14.7	4 ~ 5	停止分裂 Stop dividing	封闭 Closed
泰山红 Taishanhong	178.3 B	117.9 B	16 963.0 B	9.1	3	停止分裂 Stop dividing	封闭不严 Not tightly closed
突尼斯软籽 Tunisian Soft Seed	220.3 AB	283.9 A	65 611.1 A	21.1	3 ~ 4	仍在分裂 Still dividing	封闭不严 Not tightly closed

温度/℃ Temperature	泰山红 Taishanhong	鲁青1号 Luqing 1	突尼斯软籽 Tunisian Soft Seed
5	100.00	100.00	100.00
0	100.00	100.00	100.00
-5	100.00	94.40	100.00
-10	93.33	100.00	94.12
-15	10.00	93.75	11.11

温度 / ℃ Temperature	电导率 Electrical conductivity	超氧化物歧化酶SOD	过氧化物酶POD	丙二醛 MDA	可溶性糖 Soluble sugar	可溶性蛋白 Soluble protein
5	-0.12	-0.14	-0.76	0.92	0.70	-0.77
0	-0.14	0.62	-0.58	0.87	-0.98^*	-0.73
-5	0.99^**	0.19	-0.94	0.96^*	0.10	-0.62
-10	0.91	0.97^*	0.43	-0.78	0.45	-0.82
-15	0.93	1.00^**	0.19	1.00^**	0.32	-0.80

性状 Trait	相关系数
节间长Internode length	0.29
枝条粗Width of branch	-0.96^*
针刺长Length of branch spines	0.02
针刺粗Width of branch spines	-0.98^*
皮孔横径Transverse diameter of lenticelles	-0.73
皮孔纵径Longitudinal diameter of lenticelles	-0.49
皮孔面积Area of lenticelles	-0.29

[1]	QIN Gaihua, LIU Chunyan, LI Jiyu, and XU Yiliu, . A New Pomegranate Cultivar‘Feihong’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 37-38.
[2]	QIN Gaihua, LIU Chunyan, LI Jiyu, and XU Yiliu, . A New Pomegranate Cultivar‘Suzi’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 39-40.
[3]	WANG Sha, ZHANG Xinhui, ZHAO Yujie, LI Bianbian, ZHAO Xueqing, SHEN Yu, DONG Jianmei, YUAN Zhaohe. Cloning and Functional Analysis of PgMYB111 Related to Anthocyanin Synthesis in Pomegranate [J]. Acta Horticulturae Sinica, 2022, 49(9): 1883-1894.
[4]	HU Ruolin, WANG Jiali, YANG Huiqin, YUAN Chao, NIU Yi, TANG Qinglin, WEI Dayong, TIAN Shibing, YANG Yang, WANG Zhimin. Cloning and Functional Analysis of Auxin Response Factor Gene SmARF5 in Solanum melongena [J]. Acta Horticulturae Sinica, 2022, 49(9): 1895-1906.
[5]	WEI Xiaoyu, WANG Yuejin. Correlation Between Anatomical Structure and Resistance to Powdery Mildew in Chinese Wild Vitis Species [J]. Acta Horticulturae Sinica, 2022, 49(6): 1200-1212.
[6]	LI Xiaoming, YU Junchi, WANG Chunxia. Comparison of Growth and Secondary Metabolites of Purple and White Flower Dracocephalum moldavica Under Field,Greenhouse and Greenhouse Shading Conditions [J]. Acta Horticulturae Sinica, 2022, 49(6): 1363-1370.
[7]	ZHAO Jianrong, YANG Yuan, QIN Gaihua, LIU Chunyan, YU Qing, JIA Botao, SU Ying, CAO Zhen, LI Jiyu. Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate [J]. Acta Horticulturae Sinica, 2022, 49(4): 758-768.
[8]	GUO Xuemin, WANG Xinrui, WANG Yingying, LI Zheng, MIAO Ningning, WANG Zhaojun. Anatomical Observation on the Tortuousness of Ziziphus jujuba var. tortuosa Branches [J]. Acta Horticulturae Sinica, 2021, 48(9): 1653-1664.
[9]	NIE Weijie, WANG Chenhe, MA Wanru, SU Jiangshuo, ZHANG Fei, FANG Weimin, CHEN Sumei, CHEN Fadi, GUAN Zhiyong. Comprehensive Evaluation of Flower Branch Characteristics of Cut Chrysanthemums [J]. Acta Horticulturae Sinica, 2021, 48(6): 1150-1162.
[10]	ZHAO Yujie, LIU Cuiyu, ZHAO Xueqing, WANG Yuying, YAN Ming, YUAN Zhaohe. Cloning and Spatiotemporal Expression Analysis of PgWUS and PgBEL1 in Punica granatum [J]. Acta Horticulturae Sinica, 2021, 48(2): 355-366.
[11]	YUAN Tao, CHEN Tingqiao, TANG Ying. Secondary Branches Development of Paeonia ludlowii [J]. Acta Horticulturae Sinica, 2021, 48(1): 117-126.
[12]	GUO Yan，ZHANG Shuhang，LI Ying，ZHANG Xinfang，and WANG Guangpeng*. Studies on the Leaf Morphology，Anatomical Structure and Drought Resistance Evaluation of 238 Chinese Chestnut Varieties（Strains） [J]. ACTA HORTICULTURAE SINICA, 2020, 47(6): 1033-1046.
[13]	HU Xijun1,*，JIN Xiaoling1,2，XING Wen1，WANG Xiaoli1，and ZHANG Yaping1,3. A New Zelkova schneideriana Cultivar‘Linyuan’ [J]. ACTA HORTICULTURAE SINICA, 2020, 47(5): 1017-1018.
[14]	FENG Shuangshuang1,2，LUO Jiayi1,2，ZHU Xijian2,4，JIANG Jibin2,4，HUANG Sanwen1,3,，and ZHANG Jinzhe2,. Homozygous Mutant Construction and Function Analysis of TCP Transcription Factor StBRC1a in Diploid Potato [J]. ACTA HORTICULTURAE SINICA, 2020, 47(1): 63-72.
[15]	LI Haoxian1，CAO Shangyin1,*，YAO Fang2，LUO Xiang1，XIN Changyong3，ZHAO Diguang1，ZHANG Shuying2，XIA Xiaocong1，LIU Jun2，CHEN Lina1，YAO Hailei2，and JING Dan1. A New Soft-seed Pomegranate Cultivar‘Zhongshiliu 8’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2771-2772.