Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (7): 1574-1586.doi: 10.16420/j.issn.0513-353x.2022-0414
• Plant Protection • Previous Articles Next Articles
DENG Chengfeng1, LI Suping1, ZHANG Ruixuan1, HAN Leng2, LI Yong1,*()
Received:
2022-12-23
Revised:
2023-02-27
Online:
2023-07-25
Published:
2023-07-26
Contact:
LI Yong
DENG Chengfeng, LI Suping, ZHANG Ruixuan, HAN Leng, LI Yong. Control Effect of Lysobacter enzymogenes LE16 on Rot Disease in Post-harvest Citrus Fruit Caused by Penicillium digitatum and P. italicum[J]. Acta Horticulturae Sinica, 2023, 50(7): 1574-1586.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2022-0414
Fig. 2 Effect of self-digestive fermentation liquid of LE16 on mycelial morphology of Penicillium digitatum and P. italicum under scanning electron microscope Red arrow shows that the mycelium of the pathogen was shriveled and twisted after self-digestive fermentation liquid treated.
Fig. 4 Inhibition of self-digestive fermentation liquid of LE16 treated with different temperatures on mycelial growth of Penicillium digitatum and P. italicum
代谢物 Metabolite | 质荷比 m/z | 保留时间/min Retention time | 加合离子模式 Adducts | 分子式 Formula |
---|---|---|---|---|
米诺环素 Minocycline | 456.1778628 | 2.326 | M-H | C23H27N3O7 |
替加环素 Tigecycline | 606.2573465 | 4.017 | M+Na-2H | C29H39N5O8 |
肉桂醛 Cinnamaldehyde | 133.0645897 | 6.307 | M+H-H2O,M+H | C9H8O |
月桂酸 Dodecanoic acid | 218.2110017 | 3.517 | M+NH4 | C12H24O2 |
p-水杨酸 p-Salicylic acid | 137.0231501 | 2.304 | M-H | C7H6O3 |
肉桂酸 Cinnamic acid | 166.0859303 | 1.453 | M+H-H2O,M+NH4,M+H | C9H8O2 |
利福布丁 Rifabutin | 827.4281339 | 6.570 | M-H2O-H | C46H62N4O11 |
γ-丁内酯γ-Butyrolactone | 257.1040826 | 3.193 | 3M-H | C4H6O2 |
纳奈霉素 Nanaomycin | 301.0742449 | 6.327 | M-H | C16H14O6 |
Table 1 Preliminary analysis results of antibacterial substances of self-digestive fermentation liquid of LE16
代谢物 Metabolite | 质荷比 m/z | 保留时间/min Retention time | 加合离子模式 Adducts | 分子式 Formula |
---|---|---|---|---|
米诺环素 Minocycline | 456.1778628 | 2.326 | M-H | C23H27N3O7 |
替加环素 Tigecycline | 606.2573465 | 4.017 | M+Na-2H | C29H39N5O8 |
肉桂醛 Cinnamaldehyde | 133.0645897 | 6.307 | M+H-H2O,M+H | C9H8O |
月桂酸 Dodecanoic acid | 218.2110017 | 3.517 | M+NH4 | C12H24O2 |
p-水杨酸 p-Salicylic acid | 137.0231501 | 2.304 | M-H | C7H6O3 |
肉桂酸 Cinnamic acid | 166.0859303 | 1.453 | M+H-H2O,M+NH4,M+H | C9H8O2 |
利福布丁 Rifabutin | 827.4281339 | 6.570 | M-H2O-H | C46H62N4O11 |
γ-丁内酯γ-Butyrolactone | 257.1040826 | 3.193 | 3M-H | C4H6O2 |
纳奈霉素 Nanaomycin | 301.0742449 | 6.327 | M-H | C16H14O6 |
Fig. 5 Effects of self-digestive fermentation liquid of LE16 on disease incidence,disease severity and control effect of citrus fruit by inoculation with Penicillium italicum. Different lowercase letters indicate significant differences(P < 0.05).
处理 Treatment | 丙二醛含量/(nmol · g-1) Malondialdehyde | 细胞膜透性/% Cell membrane permeability | 超氧化物歧化酶/(U · g-1) Superoxide dismutase | 过氧化物酶/(U · g-1 · min-1) Peroxidase | 过氧化氢酶/(mg · g-1 · min-1) Catalase |
---|---|---|---|---|---|
对照Control | 5.56 ± 0.85 a | 42.91 ± 0.82 a | 39.64 ± 1.12 c | 9.58 ± 0.83 b | 2.72 ± 0.01 b |
LE16 | 4.14 ± 0.82 ab | 31.62 ± 1.31 b | 44.19 ± 1.48 b | 12.64 ± 1.19 b | 3.64 ± 0.01 a |
咪鲜胺Prochloraz | 2.70 ± 0.41 b | 32.39 ± 0.90 b | 49.70 ± 0.86 a | 13.89 ± 1.45 ab | 3.69 ± 0.02 a |
50% LE16 + 50%咪鲜胺 50% LE16 + 50% Prochloraz | 3.70 ± 0.46 ab | 36.90 ± 0.55 b | 50.09 ± 1.55 a | 18.19 ± 1.82 a | 3.77 ± 0.05 a |
Table 2 Effects in enzyme activities in citrus peel of self-digestive fermentation liquid of LE16 in the Penicillium italicum inoculation test
处理 Treatment | 丙二醛含量/(nmol · g-1) Malondialdehyde | 细胞膜透性/% Cell membrane permeability | 超氧化物歧化酶/(U · g-1) Superoxide dismutase | 过氧化物酶/(U · g-1 · min-1) Peroxidase | 过氧化氢酶/(mg · g-1 · min-1) Catalase |
---|---|---|---|---|---|
对照Control | 5.56 ± 0.85 a | 42.91 ± 0.82 a | 39.64 ± 1.12 c | 9.58 ± 0.83 b | 2.72 ± 0.01 b |
LE16 | 4.14 ± 0.82 ab | 31.62 ± 1.31 b | 44.19 ± 1.48 b | 12.64 ± 1.19 b | 3.64 ± 0.01 a |
咪鲜胺Prochloraz | 2.70 ± 0.41 b | 32.39 ± 0.90 b | 49.70 ± 0.86 a | 13.89 ± 1.45 ab | 3.69 ± 0.02 a |
50% LE16 + 50%咪鲜胺 50% LE16 + 50% Prochloraz | 3.70 ± 0.46 ab | 36.90 ± 0.55 b | 50.09 ± 1.55 a | 18.19 ± 1.82 a | 3.77 ± 0.05 a |
储藏时间/d Storage time | 处理 Treatment | 维生素C/ (mg · mL-1) Vitamin C | 还原糖/% Reducing sugar | 可溶性固形物/% TSS | 失重率/% Weight loss rate | 硬度/N Hardness |
---|---|---|---|---|---|---|
0 | 对照组Control | 0.54 ± 0.10 | 0.14 ± 0.00 | 10.43 ± 0.04 | 13.53 ± 0.44 | |
15 | 对照组Control | 0.37 ± 0.01 a | 0.14 ± 0.00 a | 10.68 ± 0.08 a | 1.62 ± 0.79 a | 12.74 ± 0.40 a |
LE16 | 0.34 ± 0.03 a | 0.13 ± 0.01 a | 10.58 ± 0.07 a | 0.61 ± 0.14 a | 13.00 ± 0.46 a | |
咪鲜胺Prochloraz | 0.39 ± 0.02 a | 0.12 ± 0.02 a | 10.70 ± 0.03 a | 0.48 ± 0.04 a | 13.49 ± 0.45 a | |
50% LE16 + 50%咪鲜胺50% LE16 + 50% Prochloraz | 0.38 ± 0.01 a | 0.16 ± 0.01 a | 10.75 ± 0.03 a | 0.54 ± 0.09 a | 13.42 ± 0.39 a | |
30 | 对照组Control | 0.42 ± 0.02 a | 0.16 ± 0.01 a | 11.00 ± 0.16 a | 5.80 ± 2.62 a | 10.61 ± 0.21 a |
LE16 | 0.42 ± 0.02 a | 0.18 ± 0.01 a | 10.77 ± 0.12 a | 2.91 ± 1.35 a | 10.09 ± 0.32 a | |
咪鲜胺Prochloraz | 0.42 ± 0.02 a | 0.19 ± 0.00 a | 10.15 ± 0.11 a | 2.43 ± 1.36 a | 10.36 ± 0.46 a | |
50% LE16 + 50%咪鲜胺50% LE16 + 50% Prochloraz | 0.42 ± 0.02 a | 0.16 ± 0.01 a | 10.75 ± 0.10 a | 1.59 ± 1.37 a | 10.82 ± 0.12 a |
Table 3 Effect of self-digestive fermentation liquid of LE16 on quality of citrus fruits during storage
储藏时间/d Storage time | 处理 Treatment | 维生素C/ (mg · mL-1) Vitamin C | 还原糖/% Reducing sugar | 可溶性固形物/% TSS | 失重率/% Weight loss rate | 硬度/N Hardness |
---|---|---|---|---|---|---|
0 | 对照组Control | 0.54 ± 0.10 | 0.14 ± 0.00 | 10.43 ± 0.04 | 13.53 ± 0.44 | |
15 | 对照组Control | 0.37 ± 0.01 a | 0.14 ± 0.00 a | 10.68 ± 0.08 a | 1.62 ± 0.79 a | 12.74 ± 0.40 a |
LE16 | 0.34 ± 0.03 a | 0.13 ± 0.01 a | 10.58 ± 0.07 a | 0.61 ± 0.14 a | 13.00 ± 0.46 a | |
咪鲜胺Prochloraz | 0.39 ± 0.02 a | 0.12 ± 0.02 a | 10.70 ± 0.03 a | 0.48 ± 0.04 a | 13.49 ± 0.45 a | |
50% LE16 + 50%咪鲜胺50% LE16 + 50% Prochloraz | 0.38 ± 0.01 a | 0.16 ± 0.01 a | 10.75 ± 0.03 a | 0.54 ± 0.09 a | 13.42 ± 0.39 a | |
30 | 对照组Control | 0.42 ± 0.02 a | 0.16 ± 0.01 a | 11.00 ± 0.16 a | 5.80 ± 2.62 a | 10.61 ± 0.21 a |
LE16 | 0.42 ± 0.02 a | 0.18 ± 0.01 a | 10.77 ± 0.12 a | 2.91 ± 1.35 a | 10.09 ± 0.32 a | |
咪鲜胺Prochloraz | 0.42 ± 0.02 a | 0.19 ± 0.00 a | 10.15 ± 0.11 a | 2.43 ± 1.36 a | 10.36 ± 0.46 a | |
50% LE16 + 50%咪鲜胺50% LE16 + 50% Prochloraz | 0.42 ± 0.02 a | 0.16 ± 0.01 a | 10.75 ± 0.10 a | 1.59 ± 1.37 a | 10.82 ± 0.12 a |
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