Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (11): 2227-2238.doi: 10.16420/j.issn.0513-353x.2020-0952

• Original article • Previous Articles     Next Articles

Identification and Expression Analysis of miR156/157-SPL Pathway Genes in Cucumber

WANG Shuwen, YANG Aiyi, WANG Huasen(), XU Yunmin()   

  1. Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province,College of Horticulture Science,Zhejiang A & F University,Hangzhou 311300,China
  • Received:2021-07-30 Revised:2021-10-13 Published:2021-12-02
  • Contact: WANG Huasen,XU Yunmin;


In this study,Cucumis sativus‘9930’was used as the material. The cucumber phenotype during different stages,the annotation of miR156/157-SPL,the structure and expression of genes were analyzed. The results showed cucumber exhibited an age-dependent developmental pattern,the leaf 1/2,leaf 3/4 and leaf 5 stages were classified as the juvenile stage,transition stage and adult stage,respectively. In cucumber genome,seven Csa-MIR156 loci,three Csa-MIR157 loci and 14 Csa-SPL were identified and distributed on chromosome 1,2,3,4 and 6,respectively. Exon number of Csa-SPL span is from 2 to 10,and their SBP domains are coded by exon 1 and 2. SBP domains contain two zinc finger domains (Zn_1 and Zn_2)and a nuclear localization signal. In addition,the large molecular weight of Csa-SPL8 and Csa-SPL11 contain several additional domains. Csa-SPL were clustered into 5 sub-family by phylogenetic tree analysis. Eleven Csa-SPL were predicted as target of Csa-miR156/157,and three of them (Csa-SPL5,10 and 14)hold the Csa-miR156/157 binding sites within the 3′UTR. Csa-SPL7,as a miR156 target,holds a specific mutation in 11th position. Interesting,this mutation is conserved among Cucurbitaceae plants. Gene detection results showed Csa-MIR156B and C functioned as the major loci,the expression of Csa-miR156 decreased and its Csa-SPL targets increased during age development. Specifically,Csa-SPL7 was highly expressed in cotyledon stage and its abundance showed no change during plant development. The expression pattern of Csa-SPL7 was consisted with the mutation within Csa-miR156 binding site,which suggested negative regulation by Csa-miR156 was relieved in Csa-SPL7. In summary,this study suggests miR156/157-SPL pathway plays a role in regulating cucumber vegetative traits(leaf shape,internode length and tendril formation),moreover,the releasing of SPL7 may play an unknown role during early vegetative stage in Cucurbitaceae plants.

Key words: cucumber, Csa-miR156/157, Csa-SPL, gene expression

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