ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (5): 877-890.doi: :10.16420/j.issn.0513-353x.2018-0438

• Research Papers • Previous Articles     Next Articles

A Pyrimidine Derivative Confers Cucumber Induced Resistance to Fusarium Wilt

SHI Yanxia1,ZHANG Xiaohui1,XU Yufang2,XIE Xuewen1,CHAI Ali1,and LI Baoju1,*   

  1. 1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China
  • Online:2019-05-25 Published:2019-05-25


In this study,a new type of disease-resistant activator named BDO-1,(E)-N-[2-fluoro-4-(trifluoromethyl)styryl]-1-met-hyl-1H-pyrazolo[3,4-d]pyrimidin-4-amine,which belongs to pyrazolium pyrimidine derivative was reported and studied. To reveal the resistance mechanism of BDO-1,the induced resistance effect of the activator to cucumber against Fusarium oxysporum f. sp. cucumerinum was investigated. The antifungal activity of BDO-1 was detected by using plate growth rate method,and the result showed that BDO-1 had no antagonistic effect against F. oxysporum. The leaves were treated with 10 mg · L-1 BOD-1 by foliar spraying method at two-leaf and one-hearted cucumber stage. A total of five times were induced in 25 days and the pathogen F. oxysporum was inoculated after 24 h of last induction.The induced assay results indicated that the induced resistance effect of BDO-1 to cucumber against F. oxysporum was 53.28%. In addition,laser confocal microscopy confirmed that the infection rates of F. oxysporum on cucumber were inhibited by induction of BDO-1. Meanwhile,fluorescence microscopy showed that the secondary metabolite callose accumulated in cucumber plants on the 3rd day post-inoculation,and the highest content of anti-oxidant H2O2 appeared in cucumber plants on the 1st day post-inoculation. Besides,qRT-PCR revealed that BDO-1 could induce expression of six disease-resistant defense genes. After BDO-1 induction,the expression levels of CAT,PAL,LOX and EIN2 in the defense genes of root defense tissues increased significantly from 1st day to 7th day post-inoculation,and the expression levels of PR1 and SOD were higher at 1st day post-inoculation,significantly reduced in 3rd day and 7th day post-inoculation. This study provides an insight and basis for the development and application of BDO-1 as a new type of disease-resistant activator.

Key words: cucumber, pyrazolopyrimidine derivative, cucumber wilt disease, induced disease resistance

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