Establishment and Application of Loop-mediated Isothermal Amplification on Citrus psorosis virus

doi:10.16420/j.issn.0513-353x.2018-0840

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (7): 1409-1416.doi: 10.16420/j.issn.0513-353x.2018-0840

• New Technologies and New Methods • Previous Articles Next Articles

Establishment and Application of Loop-mediated Isothermal Amplification on Citrus psorosis virus

LI Min，ZHOU Tianyu，WU Jiaxing，ZHOU Yan，CAO Mengji*，and LI Zhongan*

Citrus Research Institute，Southwest University，Chinese Academy of Agricultural Sciences，Chongqing 400712，China

Online:2019-07-25 Published:2019-07-25

Abstract

Abstract: Four sets of specific primer pairs in the conserved region of the CP（coat protein）gene of Citrus psorosis virus（CPsV）were designed for One-step RT-LAMP detection system based on NCBI database. One feasible pair of primers was screened to be suitable for this system. Template RNA extracted from CPsV infected citrus leaves was used for One-step RT-LAMP，and isothermal conditions were optimized to be 60 ℃ for 60 minutes. The agarose gel electrophoresis analysis of RT-LAMP products indicated that the established RT-LAMP system was specific and sensitive in detecting CPsV and 10-fold higher than RT-PCR method in sensitivity. Of 87 samples detected by this RT-LAMP system，five were found to be infected by CPsV，showing the detection result was consistent with RT-PCR method. Hence this RT-LAMP system is applicable for rapid and accurate detection and identification of CPsV.

Key words: citrus, Citrus psorosis virus（CPsV）, RT-LAMP, detection

CLC Number:

S 666

LI Min，ZHOU Tianyu，WU Jiaxing，ZHOU Yan，CAO Mengji*，and LI Zhongan*. Establishment and Application of Loop-mediated Isothermal Amplification on Citrus psorosis virus[J]. ACTA HORTICULTURAE SINICA, 2019, 46(7): 1409-1416.

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Establishment and Application of Loop-mediated Isothermal Amplification on Citrus psorosis virus

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