Molecular Characterization of a Transcription Factor BrWRKY75 Related to Leaf Senescence of Chinese Flowering Cabbage

doi:10.16420/j.issn.0513-353x.2016-0231

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (6): 1089-1098.doi: 10.16420/j.issn.0513-353x.2016-0231

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Molecular Characterization of a Transcription Factor BrWRKY75 Related to Leaf Senescence of Chinese Flowering Cabbage

TAN Xiao-li，FAN Zhong-qi，LI Lu-lu，WU Ya，KUANG Jian-fei，LU Wang-jin，and CHEN Jian-ye*

（State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources，Engineering Research Center of Southern Horticultural Products Preservation，Ministry of Education，Guangdong Key Laboratory for Postharvest Science，College of Horticultural Science，South China Agricultural University，Guangzhou 510642，China）

Online:2016-06-25 Published:2016-06-25

Abstract

Abstract:

In the present work，a WRKY transcription factor，termed BrWRKY75 was isolated from Chinese flowering cabbage. Analysis of deduced amino acid sequence and phylogenetic tree found that BrWRKY75 showed high homology with Brassica oleracea WRKY75 and Arabidopsis thaliana WRKY75，and belong to Ⅱ c sub-group. Real-time quantitative PCR displayed that BrWRKY75 was up-regulated during leaf senescence of Chinese flowering cabbage. Sub-cellular localization and transcriptional activity analysis revealed that BrWRKY75 is a nuclear protein with transcriptional repression activity. In addition，electrophoretic mobility shift assay（EMSA）confirmed that BrWRKY75 directly bound to the W-box（TTGAC）cis-element. Taken together，these results provide basis for the further investigation of the transcriptional regulation mechanism of Chinese flowering cabbage leaf senescence.

Key words: Chinese flowering cabbage, leaf senescence, WRKY transcription factor, transcriptional regulation

CLC Number:

S 634.5

TAN Xiao-li，FAN Zhong-qi，LI Lu-lu，WU Ya，KUANG Jian-fei，LU Wang-jin，and CHEN Jian-ye*. Molecular Characterization of a Transcription Factor BrWRKY75 Related to Leaf Senescence of Chinese Flowering Cabbage[J]. ACTA HORTICULTURAE SINICA, 2016, 43(6): 1089-1098.

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Molecular Characterization of a Transcription Factor BrWRKY75 Related to Leaf Senescence of Chinese Flowering Cabbage

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