Gene Cloning and Expression Analysis of Three Aux/IAA Genes in Eggplant

doi:10.16420/j.issn.0513-353x.2015-0338

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (10): 2049-2058.doi: 10.16420/j.issn.0513-353x.2015-0338

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Gene Cloning and Expression Analysis of Three Aux/IAA Genes in Eggplant

ZHANG Li-hui1,*，TIAN Shi-bing2,*，WANG Zhi-min1,**，AN Li-yu1，TANG Qing-lin1，WANG Yong-qing2，YANG Yang2，and SONG Ming1,**

1College of Horticulture and Landscape Architecture，Southwest University，Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education，Chongqing Key Laboratory of Olericulture，Chongqing 400715，China；2The Institute of Vegetables and Flowers，Chongqing Academy of Agricultural Sciences，Chongqing 400055，China

Online:2015-10-25 Published:2015-10-25

Abstract

Abstract:

Three Aux/IAA genes SmIAA3，SmIAA4 and SmIAA9 in eggplant were cloned from parthenocarpic eggplant‘D-7-1’. The full CDS length of SmIAA3，SmIAA4 and SmIAA9 was 573，564 and 915 bp，which encoded three polypeptides of 190，187 and 304 amino acids. The phylogenetic analysis showed that the three proteins belonged to Aux/IAA family proteins and they had the highest similarity with Aux/IAA proteins of tomato. And the three novel proteins contained conserved domainⅠ，Ⅱ，Ⅲ and Ⅳ of Aux/IAA family proteins. The molecular weight of the three proteins were 21.50，20.95 and 32.67 kD with isoelectric point 7.62，6.02 and 8.57 respectively. The RT-PCR analysis showed that the expression of SmIAA9 gene was different between parthenocarpic and nonparthenocarpic eggplant. Evidently the parthenocarpic was higher than the nonparthenocarpic before flowering，which indicated thatSmIAA9 would be a gene related with parthenocarpy.

Key words: eggplant, parthenocarpy, Aux/IAA, cloning, bioinformaticanalysis, expression analysis

CLC Number:

S 641.1

ZHANG Li-hui1,*，TIAN Shi-bing2,*，WANG Zhi-min1,**，AN Li-yu1，TANG Qing-lin1，WANG Yong-qing2，YANG Yang2，and SONG Ming1,**. Gene Cloning and Expression Analysis of Three Aux/IAA Genes in Eggplant[J]. ACTA HORTICULTURAE SINICA, 2015, 42(10): 2049-2058.

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Gene Cloning and Expression Analysis of Three Aux/IAA Genes in Eggplant

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