Abstract: Pollens gains from 31 Nelumbo germplasm was examined using scanning electron microscopy（SEM）. The results showed that all pollen grains investigated are sub-spheroidal in shape and elliptical in equatorial view. Pollen are predominately 3-lobed-rounder type in polar view，and the others are sub-orbicular. The pollen of all the accessions exclusively are 3-colpate type，and five accessions are additional 6-pantocolpate type. The obvious differences of pollen morphology of those 31 Nelumbo germplasm，especially in the characters of colpus and lirae demonstrated the genetic diversity and divergence，which provided valuable tools to analyze the phylogenetic relationship among the Nelumbo accessions.

Key words: lotus, Nelumbo, pollen morphology, germplasm resource, genetic diversity, scanning electron microscopy（SEM）