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园艺学报 ›› 2019, Vol. 46 ›› Issue (1): 96-.doi: 10.16420/j.issn.0513-353x.2018-0225

• 研究论文 • 上一篇    下一篇

茶树蛋白激酶基因CsCIPK的克隆与表达特性分析

刘 昊,王文丽,滕瑞敏,李 辉,王 瑜,王永鑫,庄 静*   

  1. (南京农业大学园艺学院,茶叶科学研究所,南京 210095)
  • 出版日期:2019-01-25 发布日期:2019-01-25

Cloning and Profiles Analysis of CsCIPK Gene from Tea Plant

LIU Hao,WANG Wenli,TENG Ruimin,LI Hui,WANG Yu,WANG Yongxin,and ZHUANG Jing*   

  1. (College of Horticulture,Tea Science Research Institute,Nanjing Agricultural University,Nanjing 210095,China)
  • Online:2019-01-25 Published:2019-01-25

摘要: 以茶树品种‘龙井43’作为材料,利用RT-PCR方法,从茶树的cDNA中克隆得到1个编码蛋白激酶的基因,命名为CsCIPK。序列分析表明,CsCIPK开放阅读框长度为1 341 bp,编码446个氨基酸,蛋白质分子量为414 234。蛋白功能域预测和多重对比显示,CsCIPK蛋白含有1个保守的N端激酶结构域和1个相对不保守的C端调节结构域,即丝氨酸/苏氨酸激酶结构域和NAF结构域。理化性质、亲/疏水性、无序化分析显示,CsCIPK属于疏水性蛋白,理论等电点为7.04,有4段无序化区域,其二级结构分析显示主要由α螺旋、不规则卷曲组成。通过实时荧光定量PCR对‘龙井43’和‘安吉白茶’中的CsCIPK表达特性进行分析。结果显示‘龙井43’中CsCIPK的相对表达量在高温、干旱及盐处理4 h、低温处理24 h时达到最高。‘安吉白茶’中CsCIPK的相对表达量在高温及盐处理4 h、低温及干旱处理1 h时达到最高。CsCIPK在‘龙井43’的根中,‘安吉白茶’茎中表达量最高。不同浓度的GA和IBA处理‘龙井43’茶苗,结果显示0.2 mmol ? L-1 GA处理后,CsCIPK表达量先升高后下降,6 d时处理组为对照组的62倍;0.6 mmol ? L-1 IBA处理后,CsCIPK的表达量在3 d时显著高于对照组;不同浓度GA和IBA处理后,9 d时CsCIPK表达量均显著低于对照。

关键词: 茶树, 蛋白激酶, CsCIPK, 组织表达, 非生物胁迫, 激素处理

Abstract: In this experiment,a CIPK gene was cloned from tea plant(Camellia sinensis)cultivar‘Longjing 43’by RT-PCR,and named CsCIPK. Sequence analysis showed that the open reading frame of CsCIPK is 1 341 bp and encoded 446 amino acids with molecular weight of 414 234. The prediction of protein functional domain showed that the CsCIPK protein contains a conserved N-terminal kinase domain and a relatively unconserved C-terminal regulatory domain,namely the serine/threonine kinase domain and the NAF domain. CsCIPK is a hydrophobic protein and theoretical pI is 7.04. CsCIPK contains four unordered regions,and mainly composed of α-helix and irregular coils. The expression profile of CsCIPK gene in tea plant cultivars‘Longjing 43’and‘Anjibaicha’was analyzed by real-time quantitative PCR. The results showed that the relative expression of CsCIPK in‘Longjing 43’reached its highest level at high temperature,drought,salt treatment for 4 h,and low temperature treatment for 24 h. The relative expression of CsCIPK in‘Anjibaicha’was highest at high temperature and salt treatment for 4 h,and at low temperature and drought treatment for 1 h. In different tea tree tissues,the expression of CsCIPK was highest in the root of‘Longjing 43’,and in the stem of‘Anjibaicha’. Different concentrations of GA and IBA treatment showed that the expression level first increased and then decreased under 0.2 mmol ? L-1 GA,and reached the highest level at 6 days. After treatment with 0.6 mmol ? L-1 IBA,the expression level was significantly higher than the control at 3 days. After treatment with different concentrations of different hormones,the expression level of CsCIPK on the 9 days in the treatment was significantly lower than the control.

Key words: Camellia sinensis, protein kinase, CsCIPK, tissue expression, abiotic stress, hormonal treatment

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