https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2017, Vol. 44 ›› Issue (7): 1309-1318.doi: 10.16420/j.issn.0513-353x.2017-0099

• 研究论文 • 上一篇    下一篇

萝卜Ogura-CMS 育性恢复基因Rfo 的高通量SNP 标记开发及其应用

王庆彪,张 丽*,温常龙,杨静静   

  1. 北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,蔬菜种质改良北京市重点实验室,北京 100097
  • 出版日期:2017-07-25 发布日期:2017-07-25

Development and Application of High-throughput SNP Markers for Ogura-CMS Fertility Restorer Gene in Radish

WANG Qingbiao,ZHANG Li*,WEN Changlong,and YANG Jingjing   

  1. Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology
    and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture,Beijing Key Laboratory of
    Vegetable Germplasm Improvement,Beijing 100097,China
  • Online:2017-07-25 Published:2017-07-25

摘要:

利用萝卜Ogura-CMS 保持系和恢复系育性恢复基因Rfo 的序列,开发了基于高通量竞争性
等位基因特异性PCR(KASP)分型技术的SNP 标记:Rfo-SNP1。利用该标记对24 个萝卜品种的304 个
单株进行了育性恢复基因Rfo 的基因型鉴定,其中289 株获得基因分型结果,占整个群体的95.07%。从
分型结果看,育性恢复基因Rfo 的基因型为rfrf 的有110 株(占36.18%),基因型为RfRf 和Rfrf 的分别为
156 株(占51.32%)和23 株(占7.57%)。在12 个品种中获得基因型为rfrf 的单株,可作为保持系并用
于不育系转育。利用PCR-RFLP 标记和回交群体单株育性鉴定,进一步证实Rfo-SNP1 标记不但能够实现
高通量检测,而且能够实现萝卜Ogura-CMS 育性恢复基因Rfo 基因型的精准鉴定。目前利用该方法已成
功转育了6 套稳定遗传的雄性不育系及保持系。

关键词: 萝卜, Ogura-CMS, 育性恢复基因Rfo, SNP 标记, 辅助选择

Abstract:

Based on the nucleotide sequences of Rfo related to the Ogura-CMS fertility restorer gene
in radish,the SNP(Single nucleotide polymorphism)marker named Rfo-SNP1 was developed by use of
KASP(kompetitive allele specific PCR)technology. The genotypes of fertility restorer gene in 24 radish
varieties including 304 plants were identified using the Rfo-SNP1 marker. The results showed that 289
plants were screened out accounting for 95.07% of the whole population. And 110 plants had the recessive
homozygous genotype(rfrf)taking up 36.18%. The dominant homozygous genotype(RfRf)and the
heterozygous genotype(Rfrf)appeared respectively in 156 plants and 23 plants. In 23 fertile varieties,12
varieties had the rfrf genotype,which implied that the 12 varieties might be the candidates of maintainer
lines for breeding sterile lines. The accuracy of Rfo-SNP1 markers was further verified by PCR-RFLP and backcross populations. Six male sterile lines and maintainer lines of radish have been developed through
this procedure.

Key words: radish, Ogura-CMS, fertility restorer gene Rfo, SNP marker, assisted selection