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园艺学报 ›› 2017, Vol. 44 ›› Issue (7): 1263-1274.doi: 10.16420/j.issn.0513-353x.2017-0103

• 研究论文 • 上一篇    下一篇

嫁接对柑橘microRNA 表达的影响

徐媛媛1,朱世平1,刘晓纳1,李青萍2,赵晓春1,*   

  1. 1 西南大学,中国农业科学院柑桔研究所,国家柑橘工程技术研究中心,重庆400712;2 西南大学园艺园林学院,
    重庆400715
  • 出版日期:2017-07-25 发布日期:2017-07-25

Effects of Grafting on MicroRNAs Expression in Citrus

XU Yuanyuan1,ZHU Shiping1,LIU Xiaona1,LI Qingping2,and ZHAO Xiaochun1,*   

  1. 1Citrus Research Institute,Southwest University,Chinese Academy of Agricultural Sciences,National Citrus Engineering
    Research Center,Chongqing 400712,China;2College of Horticulture and Landscape Architecture,Southwest University,
    Chongqing 400715,China
  • Online:2017-07-25 Published:2017-07-25

摘要:

以‘锦橙’、‘资阳香橙’、‘飞龙枳’实生苗和‘锦橙’/‘资阳香橙’、‘锦橙’/‘飞龙枳’嫁接
苗为试材,通过荧光定量PCR 检测miRNA 及其靶基因在嫁接苗和实生苗叶片和根系中的表达差异,分
析嫁接对柑橘microRNAs 及其靶基因表达的影响。结果证明嫁接对柑橘miRNA 的表达有直接的影响。
嫁接的影响更多地是促进接穗中一些与调控植物生长发育、胁迫应答及激素信号转导相关的miRNA 的表
达,并抑制了其对应靶基因的表达;而在砧木中,嫁接对根系的影响较多表现为抑制与植物生长发育、
胁迫应答相关的miRNA 的表达,促进其靶基因的表达。受影响的miRNA 的种类及其表达的差异水平在
不同砧木间有明显差异。

关键词: 柑橘, 砧木, 接穗, microRNA

Abstract:

microRNAs play the important roles in regulation of plant growth and development,
responding to biotic and abiotics tresses,and signal transduction. In this study,the seedlings of‘Jincheng’
orange(Citrus sinensis Osbeck),‘Ziyang Xiangcheng’(C. junos Sied. ex Tanaka),and Flying dragon
(Poncirus trifoliata Raf.),and the grafted plants of‘Jincheng/Ziyang Xiangcheng’,‘Jincheng/Flying
Dragon’were used as materials to analyze the expression profiles of microRNAs and their target genes
before and after grafting. Fluorescence quantitative PCRs were performed to compare the expression of
miRNAs and their target genes in the leaves and roots to identify the difference on miRNA expression
between scion and rootstock. The results indicated that the grafting significantly affected the expression of
microRNAs and their target genes in both scions and rootstocks. In the leaves of scion,effect of grafting
more likely prompted the expression of microRNAs and suppressed their target genes expression involved
in the regulation of plant growth and development,stress response and hormone signal transduction. In the
roots of rootstock,effect of grafting tended to suppress the expression of miRNAs related to the regulation of plant growth and development,and stress response. The varieties of microRNAs and the level of
expression were significantly different between the two rootstocks.

Key words: citrus, rootstock, scion, microRNAs