https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2014, Vol. 41 ›› Issue (8): 1554-1562.

• 果树 • 上一篇    下一篇

枇杷7个脱水素基因表达差异分析

徐红霞1,2,李晓颖2,陈俊伟2,冯健君3,徐昌杰1,*   

  1. (1浙江大学农业与生物技术学院,农业部园艺作物生长发育和品质生物学实验室,杭州 310058;2浙江省农业科学院园艺研究所,杭州 310021;3浙江省宁海县农林局,浙江宁海 315600)
  • 收稿日期:2014-04-22 出版日期:2014-08-25 发布日期:2014-08-25

Expression Analysis of Seven Dehydrin Genes in Loquat

XU Hong-xia1,2,LI Xiao-ying2,CHEN Jun-wei2,FENG Jian-jun3,and XU Chang-jie1,*   

  1. (1The State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development and Quality Improvement,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China;2Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;3Department of Agricultural and Forestry of Ninghai County,Ninghai,Zhejiang 315600,China)
  • Received:2014-04-22 Online:2014-08-25 Published:2014-08-25

摘要: 为进一步明确枇杷(Eriobotrya japonica Lindl.)中不同脱水素基因家族成员(EjDHN)的功能和响应机制,以白沙枇杷品种‘宁海白’为试材,分析了7个EjDHN基因在不同器官、果实发育期及ABA和干旱胁迫条件下的表达模式。结果表明,EjDHN5在各器官中表达量都比较高,EjDHN4主要在花中表达,EjDHN2EjDHN3、EjDHN6EjDHN7主要在茎中表达。在果实发育过程中,除EjDHN3外,其他家族成员均与种子发育有关;而EjDHN2EjDHN5在果肉中表达量较高且与果肉发育有关。7个EjDHN基因在枇杷叶片中的季节性表达变化趋势基本一致,均在冬季表达量最高,而EjDHN1EjDHN2EjDHN4EjDHN5在初夏也有较高的表达量。在干旱胁迫后,叶片中7个EjDHN基因的表达均出现显著增强趋势,其中EjDHN6增幅最大。各基因对ABA处理也有不同响应,以EjDHN6最为敏感。

关键词: 枇杷, 脱水素, 基因表达, 果实发育期, 季节性变化, 干旱胁迫, ABA

Abstract: In order to further reveal the possible functions of different dehydrin family members in loquat(Eriobotrya japonica Lindl.)(EjDHN),the expression patterns of seven EjDHNs were analyzed with various organs,fruit at different development stages,leaves from different seasons,leaves under drought stress and ABA treatment in cultivar‘Ninghaibai’. It was observed that EjDHN5 was highly expressed in all organs tested,EjDHN4 was mainly expressed in flower,while EjDHN2EjDHN3EjDHN6 and EjDHN7 were expressed especially high in stem. In fruit,all members,only except for EjDHN3,responded to seed development. The expression levels of EjDHN2 and EjDHN5 were relatively high in pulp and responsed to pulp development. In leaves during seasonal changes,the expression pattern of different EjDHN members was rather similar,with the highest transcript levels reached in winter,whereas the expression were also strong in early summer for EjDHN1EjDHN2EjDHN4 and EjDHN5. Under drought stress,the transcript levels of all seven genes increased in leaves,with EjDHN6 most greatly increased. Moreover,expression of all these genes responded differently,with EjDHN6 most sensitive,to ABA treatment.

Key words: loquat, dehydrin, gene expression, fruit developmental stage, seasonal changes, drought stress, ABA

中图分类号: