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园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1601-1606.

• 蔬菜 • 上一篇    下一篇

钙对弱光胁迫下番茄叶片保护酶活性及可溶性蛋白含量的影响

李天来*,李益清   

  1. (沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,沈阳 110161)
  • 收稿日期:2008-05-22 修回日期:2008-10-07 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 李天来

Effects of Calcium on Activities of Protective Enzymes and Soluble Protein Content in the Leaves of Tomato under Low Light Stress

LI Tian-lai*;LI Yi-qing
  

  1. (College of Horticulture, Shenyang Agricultural University, Liaoning Province Key Laboratory of Protected Horticulture, Shenyang 110161, China)
  • Received:2008-05-22 Revised:2008-10-07 Online:2008-11-25 Published:2008-11-25
  • Contact: LI Tian-lai

摘要:

为明确Ca2+对番茄耐弱光特性的调控作用,以番茄(Lycopersicon esculentum Mill)耐弱光品系W为试材,研究了弱光(50%自然光强)胁迫条件下,钙和钙加IAA处理对叶片保护酶活性以及可溶性蛋白质含量的调控作用。结果表明:弱光胁迫下(实测光通量密度为556.8~656.6μmol·m-2·s-1)27mmol·L-1的CaCl2处理可显著降低番茄叶片MDA含量,提高SOD、CAT、POD的活性及可溶性蛋白含量,使这些指标达到正常光照下的对照植株水平;IAA进一步增强了Ca2+的这些作用。Ca2+的这些作用可能是其提高番茄植株弱光耐受性、防止植株弱光伤害的重要原因之一。

关键词: 番茄, 钙, 弱光胁迫, 保护酶活性, 可溶性蛋白

Abstract:

In order to investigate the regulating mechanism of calcium on tomato low light tolerance, leaves of one tomato low light tolerance strain W (Lycopersicon esculentum Mill) were used to study the changes of protective enzymes activities and soluble protein content in leaves after the tomato were treated by calcium or calcium and IAA under the 50% nature light intensity (identical radiant flux of 556.8 to 656.6 μmol·m-2·s-1). The results showed that 27 mmol·L-1 CaCl2 treatment could significantly decrease MDA content, increase the activities of SOD,CAT,and POD, promote the content of soluble protein in the leaves of tomato under low light stress reach the control level under 100% nature light intensity; auxin could promote the effect of Ca2+ further. These results indicated that the effects of calcium on the activities of protective enzymes and the content of soluble protein in the leaves of tomato under low light stress maybe one of the important reasons for that calcium could increased low light tolerance of tomato and prevented low light damage to the plant.

Key words: tomato, calcium, low light stress, activities of cell defense enzymes, soluble protein

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