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园艺学报 ›› 2008, Vol. 35 ›› Issue (8): 1117-1124.

• 果树 • 上一篇    下一篇

山楂(Crataegus pinnatifida Bge.)遗传多样性的RAPD和ISSR标记分析

代红艳1,2;郭修武1,2; 张 叶1;李媛媛1,2;李 贺1,2;周传生2;张志宏1,2*
  

  1. 1沈阳农业大学果树生物技术与遗传改良创新团队,沈阳110161;2沈阳农业大学园艺学院,沈阳110161)
  • 收稿日期:2008-02-03 修回日期:2008-06-11 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 张志宏

Genetic Diversity of Crataegus pinnatifida Bge. as Evaluated by ISSR and SSR Markers

DAI hong-yan1,2, GUO Xiu-wu1,2, ZHANG Ye1, LI Yuan-yuan1,2, LI He1, ZHOU Chuan-sheng2, and ZHANG Zhi-hong1,2*
  

  1. 1Team of Biotechnology and Genetic Improvement of Fruit Trees, Shenyang Agricultural University, Shenyang 110161, China; 2College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China)
  • Received:2008-02-03 Revised:2008-06-11 Online:2008-08-25 Published:2008-08-25
  • Contact: ZHANG Zhi-hong

摘要:

利用RAPD和ISSR标记对35份山楂(Crataegus pinnatifida Bge.)资源进行了DNA多态性分析。12个RAPD引物共扩增出110条清晰的谱带,其中89条显示多态性,平均每个引物扩增出7.4条多态性谱带。13个ISSR引物共扩增出110条清晰的谱带,其中94条显示多态性,平均每个引物扩增出7.2条多态性谱带。基于RAPD和ISSR标记,利用UPGMA分别构建了35份山楂资源的聚类树状图。距离系数分别为0~0.62(RAPD)和0~0.64(ISSR),表明山楂具有较高的遗传多样性。

关键词: 山楂, RAPD, ISSR, 遗传多样性

Abstract:

The DNA polymorphism of thirty-five hawthorn accessions (Crataegus pinnatifida Bge.) was analyzed using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers in the present study. Twelve RAPD primers generated a total of 110 clear and reproducible bands, of which 89 were polymorphic, with an average of 7.4 polymorphic fragments per primer. Thirteen ISSR primers generated a total of 110 clear and reproducible bands, of which 94 were polymorphic, with an average of 7.2 polymorphic fragments per primer. The dendrogram based on RAPD and ISSR data were constructed separately by using UPGMA analysis. The distance coefficient of the analyzed materials ranged from 0 to 0.62 and 0 to 0.64 for RAPD and ISSR markers, respectively. Our results indicated that the C. pinnatifida species has high level of genetic diversity.

Key words: Crataegus pinnatifida Bge., RAPD, ISSR, genetic diversity

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