电场诱导原生质体融合获得柑橘属间四倍体体细胞杂种

园艺学报 ›› 2002, Vol. 29 ›› Issue (4): 372-374.

电场诱导原生质体融合获得柑橘属间四倍体体细胞杂种

刘继红；胡春根；邓秀新

(华中农业大学作物遗传改良国家重点实验室, 武汉430070)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-08-25 发布日期:2002-08-25

Production of Citrus Intergeneric Tetraploid Somatic Hybrids Plant from Elec􀀁tricity-mediated Protoplast Fusion

Liu Jihong, Hu Chungen, and Deng Xiuxin

(National Key Laboratory of Crop Genetic Impr ovements, Huazhong Agricultur al University , Wuhan 430070, China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

摘要/Abstract

摘要： 采用电融合方法诱导澳洲指橘(Microcitrus papuana Swingle) 悬浮系原生质体与柑(Citrusreticulata Blanco) 叶肉原生质体融合, 得到了再生植株。染色体检查表明再生植株为四倍体( 2n= 4x= 36) , 6 个10-mer 碱基随机引物PCR 扩增结果表明, 尽管再生植株带型表现多样, 但仍然可以证明为体细胞杂种。对再生植株田间生长异常的现象进行了讨论。

关键词: 柑橘, 电融合, 体细胞杂种, 四倍体, RAPD

Abstract: Ponkan (Citrus reticulata Blanco) , which is very seedy, is one of the most widely grown mandarin cultivars in China for its good quality. Mitochondrial genome of Microcitrus papuana is related to seedlessness. But it is of difficulty to transfer the cytoplasmic genome of the latter. The present research involves electrofusing embryonic protoplasts of M. papuana and leaf derived protoplasts of Ponkan, from which plants were obtained. Chromo􀀁
some detection showed that the plants were tetraploids with 36 hromosomes. Amplification with six 10-mer arbitrary primers ident ified the plant as true somatic hybrids though complex band profiles of the regenerated plant were observed. The somatic hybrid plant holds great potential for breeding seedless varieties of Citrus . In addit ion, abnor􀀁
mal growth of the plant in field is discussed herein.

Key words: Citrus, Electrofusion, Somatic hybrid, Tetraploid, RAPD

中图分类号:

刘继红;胡春根;邓秀新. 电场诱导原生质体融合获得柑橘属间四倍体体细胞杂种[J]. 园艺学报, 2002, 29(4): 372-374.

Liu Jihong;Hu Chungen;and Deng Xiuxin. Production of Citrus Intergeneric Tetraploid Somatic Hybrids Plant from Elec􀀁tricity-mediated Protoplast Fusion[J]. ACTA HORTICULTURAE SINICA, 2002, 29(4): 372-374.

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