关键词: 百合, EST, SSR, 引物设计

Abstract: The growing availability of EST sequences from Lilium L. provides a potential valuable source of new SSR markers. In this study, 101 SSR-ESTs from 1688 ESTs in the National Center for Biotechnology Information (NCBI) database, representing 5.98% of the total number of ESTs were identified .Among them, the trinucleotide repeat is the dominant type with repeat motifs being the most common, accounting for 2.84% of ESTs. 47 kinds of repeat motifs were mined out from all SSRs. 23 SSR primers were designed to sequence flanking SSRs, the primer pairs designed were screened against genomic DNA of‘Snow Queen’from which most EST-SSRs were derived, and 18 primer pairs showed the amplification, accounting for 78.26% of total primers. Then the primers showing amplification were subjected to PCR for DNA from 13 Lilium L. cultivars of 5 hybridism series and 12 primer pairs showed polymorphisms, accounting for 66.7% of primers available. Results prove that it is an effective and feasible approach to develop SSR markers based on ESTs in Lilium L.

Key words: Lilium L., EST（expressed sequence tags）, SSR (simple sequence repeats), primer design