草原龙胆盐胁迫差减文库的构建及分析1

园艺学报 ›› 2008, Vol. 35 ›› Issue (7): 1075-1080.

草原龙胆盐胁迫差减文库的构建及分析1

王继刚¹;张坤²;徐启江¹;李玉花^1*

(¹东北林业大学生命科学学院，哈尔滨 150040；²西南林学院学报编辑部，昆明 650224)

收稿日期:2008-04-21 修回日期:2008-06-11 出版日期:2008-07-25 发布日期:2008-07-25
通讯作者: 李玉花

Construction and Analysis of Eustoma grandiflorum Subtracted cDNA library

WANG Ji-gang¹, ZHANG Kun², XU Qi-jiang¹, and LI Yu-hua^1*

(¹College of Life Science, Northeast Forestry University, Harbin 150040, China;²Editorial Department of Academic Journal, Southwest Forestry University, Kunming Yunnan 650224,China)

Received:2008-04-21 Revised:2008-06-11 Online:2008-07-25 Published:2008-07-25
Contact: LI Yu-hua

摘要/Abstract

摘要： 为了研究草原龙胆抗盐性机理，初步获得其抗盐性相关基因，分别以400 mmol·L-1的NaCl及清水处理的草原龙胆叶片mRNA为tester和driver方，利用抑制差减杂交技术构建了cDNA文库。经过测序和与GO数据库序列比对，获得了658条单一序列，其中61条序列分子功能未知，535条序列无同源性，62条序列有显著同源性。此62条序列注释按照功能分为21类。获得了活性氧清除系统基因DHAR、GST、TRX、CAT，转录因子SMT3，调控基因丝氨酸/苏氨酸激酶基因等。结果说明草原龙胆的盐抗性相关基因与数据库中的模式植物基因既有一定的共性，同时绝大部分的序列又无法用已知的基因进行解释。

关键词: 草原龙胆, 抑制差减杂交, 差异基因, 盐胁迫

Abstract: To research its' salt tolerance mechanism and to get the salt tolerance relative genes of Eustoma grandiflorum, a cDNA library was made by suppression subtractive hybridization technique. The leave mRNA of Eustoma grandiflorum treated with 400 mmol·L^-1 NaCl were tester, and that treated with pure water were driver. From the library, we got 658 unique sequences by sequencing and sequence blasting. 61 of the total sequences are functioning unknown, 535 sequences have no homology and 62 sequences have significance homology to the GO sequence library. The 62 sequences can be classified into 21 function categories. And we finally get the reactive oxygen species scavenging genes: DHAR, GST, TRX, CAT, transcription factor SMT3, and serine/threonine protein kinase gene. It indicated that there are general characters between the salt tolerance genes of Eustoma grandiflorum and model plants, and the most of the sequences can not be explained by the known genes.

Key words: Eustoma grandiflorum, suppression subtractive hybridization, differently expressed genes, salt stress

中图分类号:

S 682

王继刚;张坤;徐启江;李玉花. 草原龙胆盐胁迫差减文库的构建及分析1[J]. 园艺学报, 2008, 35(7): 1075-1080.

WANG Ji-gang;ZHANG Kun;XU Qi-jiang;and LI Yu-hua. Construction and Analysis of Eustoma grandiflorum Subtracted cDNA library[J]. ACTA HORTICULTURAE SINICA, 2008, 35(7): 1075-1080.

[1]	徐红霞, 周慧芬, 李晓颖, 姜路花, 陈俊伟. 低温胁迫下枇杷不同发育阶段的花果转录组比较分析[J]. 园艺学报, 2021, 48(9): 1680-1694.
[2]	陈皓炜, 陈梦娇, 王雅慧, 张榕蓉, 王欣蕊, 徐志胜, 谭国飞, 熊爱生. 盐胁迫下胡萝卜肉质根中木质素响应机理研究[J]. 园艺学报, 2021, 48(1): 153-161.
[3]	程少禹，宣铃娟，董彬，顾翠花，申亚梅*，张明如，戴梦怡，王卓为，章颖佳，陆丹迎. ‘红元宝’紫玉兰两次花芽分化差异代谢通路及关键调控基因筛选[J]. 园艺学报, 2020, 47(8): 1490-1504.
[4]	郑福超1，耿兴敏1,*，岳远征1，苏家乐2，贾新平2. 盐胁迫下杜鹃差异表达基因的cDNA-SCoT分析[J]. 园艺学报, 2019, 46(6): 1192-1200.
[5]	路静，马齐军，康慧，李文浩，刘亚静，郝玉金，由春香*. 苹果糖转运蛋白基因MdSWEET1在番茄中异源表达提高其耐盐性[J]. 园艺学报, 2019, 46(3): 433-443.
[6]	程贝1，王卫华1,*，吴忠东2. 非均匀盐胁迫对番茄幼苗耐盐性的影响[J]. 园艺学报, 2018, 45(5): 887-896.
[7]	段丽君，李国元*，汪殿蓓. 绒毛白蜡根区局部盐胁迫对其生长的影响[J]. 园艺学报, 2018, 45(10): 1989-1998.
[8]	吴雯雯，安玉艳，汪良驹*. 5–氨基乙酰丙酸缓解‘红颜’草莓盐胁迫伤害的时间效应研究[J]. 园艺学报, 2017, 44(6): 1038-1048.
[9]	曲丰佳，安建平，姚继芳，李浩浩，李媛媛，由春香，王小非，郝玉金. 苹果MdMYB2基因的克隆及功能鉴定[J]. 园艺学报, 2017, 44(12): 2245-2254.
[10]	齐晨辉，赵先炎，韩朋良，姜翰，王永旭，胡大刚，郝玉金. 苹果U-box型E3泛素连接酶MdPUB24的耐盐性和ABA敏感性鉴定[J]. 园艺学报, 2017, 44(12): 2255-2264.
[11]	张全艳，于建强，王佳慧，胡大刚，郝玉金. 苹果MdNAC143 的克隆及其在苹果愈伤组织的抗盐功能鉴定[J]. 园艺学报, 2017, 44(11): 2163-2170.
[12]	曾正容*，闫树堂. 柑橘衰退病晚期抑制差减cDNA文库的构建及初步分析[J]. 园艺学报, 2017, 44(10): 1871-1880.
[13]	刘亚静，马齐军，李浩浩，路静，郝玉金，由春香. 苹果MdCBL3*基因的克隆与功能鉴定[J]. 园艺学报, 2017, 44(1): 1-10.
[14]	李雅男1，袁素霞1，徐雷锋1，杨盼盼2，冯亚言1，Younes Pourbeyrami Hir1，明军1,*. 百合在NaHCO3胁迫下的生理响应机制[J]. 园艺学报, 2016, 43(6): 1126-1140.
[15]	张蕊芬，刘会，周李杰，郭颖，郝玉金，由春香. 苹果组蛋白脱乙酰化酶基因HDA19的生物信息学分析与胁迫响应研究[J]. 园艺学报, 2016, 43(4): 613-622.