园艺学报 ›› 2003, Vol. 30 ›› Issue (6): 673-677.

• 研究论文 • 上一篇    下一篇


王孝宣 杜永臣 朱德蔚 Luigi Monti2 S.Grandillo   

  1. ( 中国农业科学院蔬菜花卉研究所,北京100081; CNR-IMOF,Research Institute for Vegetable and Ornamental Plant Breed—ing,Portici 80055,Italy)
  • 收稿日期:2003-02-24 修回日期:2003-07-25 出版日期:2003-12-25 发布日期:2003-12-25

The Establishm ent of M ultiplex ——CAPS Technique and Its Application for Genetic Characterization in Tomato

W ang Xiaoxuan , Du Yongchen , Zhu Dewei , Luigi Monti2
,S. Grandillo

  1. (‘Institute ofVegetables and Flowers,Chinese Academ)ofAgricultural Science,Beijing 100081,China; CNR-IMOF,Research In—stitulefor Vegetable and Ornamental Plant Breeding,Portici 80055,haly)
  • Received:2003-02-24 Revised:2003-07-25 Online:2003-12-25 Published:2003-12-25

摘要: :应用Multiplex—PCR和CAPS(Cleaved amplified polymorphic sequence)原理研究建立了Mutiplex—
片段;(3)琼脂糖凝胶电泳溴化乙锭染色检测酶切片段长度的多态性。利用该技术对番茄(Lycopersicon es—

关键词: :番茄, 分子标记, Muhiplex—CAPS, CAPS, Multiplex—PCR

Abstract: An eficient an d reliable technique Multiplex— CAPS was developed for genetic characterization by
combination of two principles Multi—PCR and CAPS (Cleaved amplified polymorphic sequence) in tomato.This
techniqu e included (1)using 2 or 3 or more than 3 primer pairs in the same am plification reaction,after 30—40
thermal cycles the PCR yield diferent products which correspond to each individual primer pair, (2) using only
one enz、111e to digest these diferent PCR products, (3)Using agrose gel electrophoresis to detect polymorphisms
followed by ethidium staining. By comparison with CAPS, Multiplex— CAPS Can detect the polymorphism of 2 or 3
or more than 3 primer pairs in the same am plification reaction at the same time. Th e polymorphism of Multiplex—
CAPS equal exactly the polymorphism supe rimpose of each individual primer pair. Multiplex— CAPS has good re—
producibility with high an alytical efect, lOW cost, lOW labor an d time consuming.

Key words: Tomato, Multiplex— CAPS, CAPS, Multiplex—PCR