月季RcHSP17.8 基因表达提高大肠杆菌对非生物胁迫的耐性

园艺学报 ›› 2009, Vol. 36 ›› Issue (12): 1791-1798.

月季RcHSP17.8 基因表达提高大肠杆菌对非生物胁迫的耐性

蒋昌华^{1, 2} ;胡永红¹ ;徐健遥² ;张浩²;石金磊²;李敏²;王雅琼² ;明凤^2*

(¹上海植物园, 上海200231; ²复旦大学生命科学学院, 遗传工程国家重点实验室, 上海200433)

收稿日期:2009-08-19 修回日期:2009-11-27 出版日期:2009-12-25 发布日期:2009-12-25
通讯作者: 明凤

The Expression of RcHSP17.8 Gene Improving Tolerance to Abiotic Stress inRecombinant E.coli Cells

JIANG Chang-hua^{1, 2} ; HU Yong-hong¹ ; XU J ian-yao²;ZHANG Hao² ;SHI Jin-lei² ;LI Min²;WANG Ya-qiong² ; MING Feng^2*

(¹ Shanghai Botanical Garden, Shanghai 200231, China; ² State Key Laboratory of Genetic Engineering, School of Life Science,Fudan University, Shanghai 200433, China)

Received:2009-08-19 Revised:2009-11-27 Online:2009-12-25 Published:2009-12-25

摘要/Abstract

摘要： 月季RcHSP1718为编码小分子热激蛋白基因, Southern检测其在月季基因组中为多拷贝存在。38 ℃/3 h热激处理后, 该基因在耐热月季品种‘曼海姆宫殿’ ( Schloss Mannieim) 、‘彩虹’ (Radio) 、‘赌城’ (Las Vegas) 、‘梅郎随想曲’ (Caprice de Meiland) 、‘小女孩’ ( Girl) 中强表达, 而在不耐热品种‘新十全’ (Kordes’Perfecta) 、‘绯扇’ (Hiogi) 、‘莱茵黄金’ ( Pfalzer Gold) 中弱表达或不表达, 表明该基因与月季耐热性关系密切。为确定月季RcHSP1718基因功能, 将其转化E.coli BL21, 结果表明重组菌株能正常诱导表达包含RcHSP17.8的融合蛋白, 并因此提高了重组菌株对高温、低温、高盐、高pH、重金属、氧化等非生物胁迫的耐性, 表明RcHSP17.8参与了上述非生物胁迫的响应。

关键词: 月季, RcHSP1718 基因, 诱导表达, 非生物胁迫

Abstract: RcHSP17.8 is a high temperature (HT) induced expressing gene, by RT-PCR and Southern blot indicate that there are several copies of RcHSP17.8 in genome DNA of Rosa hybrida L. RcHSP17.8 is more strongly expressed in varieties resistant to HT, such as‘Schloss Mannieim’, ‘Radio’, ‘Las Vegas’,
‘Caprice de Meiland’, ‘Girl’, meanwhile weakly or not expressed in varieties sensitive to HT, such as‘Kordes’Perfecta’, ‘Hiogi’, ‘Pfalzer Gold’. The results indicate that this gene is possibly relative to the
tolerance in Rosa hybrida L. to HT. In order to study its possible function, RcHSP17.8 gene has been transferred into Escherichia coliBL21. Recombinant RcHSP17.8 was overexpressed in the recombinant E.coli cells which showed imp roved viability under different abiotic stress conditions, such as, high salt concentration,heavymetal and oxidative stress, as compared with control cultures. Those resultswould provide molecular identification principle to selecting varieties of Rosa hybrida L. with strong tolerance to HT.

Key words: Rosa hybrida L., RcHSP1718, induced expression, abiotic stress

中图分类号:

S 685.12

蒋昌华;;胡永红;徐健遥;张浩;石金磊;李敏;王雅琼;明凤. 月季RcHSP17.8 基因表达提高大肠杆菌对非生物胁迫的耐性[J]. 园艺学报, 2009, 36(12): 1791-1798.

JIANG Chang-hua;;HU Yong-hong;XU J ian-yao;ZHANG Hao;SHI Jin-lei;LI Min;WANG Ya-qiong;MING Feng. The Expression of RcHSP17.8 Gene Improving Tolerance to Abiotic Stress inRecombinant E.coli Cells[J]. ACTA HORTICULTURAE SINICA, 2009, 36(12): 1791-1798.

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