https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2004, Vol. 31 ›› Issue (6): 814-816.

• 研究报告 • 上一篇    下一篇

黄瓜前中期染色体C - 分带及其制备方法研究

钱春桃;陈劲枫*;罗向东;曹清河;雷 春
  

  1. (作物遗传与种质创新国家重点实验室, 南京农业大学园艺学院, 南京210095)
  • 收稿日期:2004-02-02 修回日期:2004-04-05 出版日期:2004-12-25 发布日期:2004-12-25

C-banding of Prometaphase Chromosomes in Cucumber and the Procedure for Slide Preparation

Qian Chuntao; Chen Jinfeng*;Luo Xiangdong; Cao Qinghe;Lei Chun
  

  1. ( State Key Laboratory of Crop Genetics and Germplasm Enhancement , College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , China)
  • Received:2004-02-02 Revised:2004-04-05 Online:2004-12-25 Published:2004-12-25

摘要: 以活体种子根为材料, 采用放线菌酮预处理, 直接获得了清晰的黄瓜前中期染色体C - 分带。
黄瓜前中期染色体的长度介于3~8μm , 单套染色体组具有21 条稳定的C - 带, 包括12 条末端带、7 条着丝点带、1 条中间带和1 条随体带。还探讨了影响前中期C - 分带制备的关键因素, 提出了黄瓜前中期染色体C - 分带的实用制备方法。

关键词: 黄瓜, 染色体, C - 分带, 前中期

Abstract: Clear C-bands at prometaphase were obtained in cucumber ( Cucumis sativus L. , 2n = 2x = 14) when cycloheximide was applied to the germinating roots in vivo as pretreatment . Chromosome length at prometaphase varied from 3 to 8μm. Haploid complement had 21 distinct and stable C-bands , including 12 telomeric - , 7 centromeric - , 1 intercary - and 1 satellite-bands. Some factors affecting the band formation were investigated and a practical procedure of C-banding of prometaphase chromosome was proposed.

Key words: Cucumber, Chromosome, C2banding, Prometaphase

中图分类号: