园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1199-1202.

• 研究论文 • 上一篇    下一篇



  1. (1 北京市农林科学院林业果树研究所, 北京100093; 2 中国农业大学园艺植物研究所, 北京100094)
  • 收稿日期:2006-04-28 修回日期:2006-09-13 出版日期:2006-12-25 发布日期:2006-12-25

Genetic Relationships of Strawberry Cultivars by AFLP Analysis

Zhang Yuntao1, 2;Feng Zhiguang2;Li Tianzhong2;Dong Jing1;Wang Guixia1;Zhang Kaichun1;Han Zhenhai2   

  1. (1 Forestry and Pomology Institute, Beijing Academ y of Agriculture and Forestry Sciences, Beijing 100093, China; 2 Institute of Horticultural Plants, China Agricultural University, Beijing 100094, China)
  • Received:2006-04-28 Revised:2006-09-13 Online:2006-12-25 Published:2006-12-25

摘要: 利用AFLP技术对我国国家草莓资源圃内保存的107个草莓品种进行了DNA多态性分析。从64对“2 + 2”引物中筛选出10对引物应用于扩增基因组DNA, 共获得清晰可辨的581条带, 其中412条为多态性带。UPGMA方法聚类分析结果显示, AFLP分子鉴定结果与其系谱关系非常一致, 表明AFLP指纹图谱分析能很好地鉴别草莓品种之间的遗传关系。

关键词: 草莓, AFLP, 亲缘关系, 分子鉴定

Abstract: The AFLP analysiswas conducted in order to investigate the blood relationship among the 107 strawberry cultivars conserved in Beijing, the National Strawberry Germplasm Repository. 10 pairs primers were selected from 64 primer pairs (2 + 2) to amplify the genomic DNAs. Total 581 AFLP fragments were obtained, 412 of them were polymorphic markers. The phylogenetic tree generated byUPGMA method showed the genetic relationship among cultivars identified by AFLP was better identical with their pedigree. AFLP technique was proved to be a very reliable method to study the genetic relationship s of strawberry cultivars.

Key words: Strawberry, AFLP, Genetic relationship, Molecular identification