https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2021, Vol. 48 ›› Issue (7): 1359-1370.doi: 10.16420/j.issn.0513-353x.2021-0194

• 研究论文 • 上一篇    下一篇

黄瓜的一个中短果突变体基因的初步定位

程凤, 宋蒙飞, 曹蕾, 张孟茹, 杨志歌, 陈劲枫, 娄群峰*()   

  1. 南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,南京 210095
  • 收稿日期:2021-03-29 修回日期:2021-05-06 出版日期:2021-07-25 发布日期:2021-08-10
  • 通讯作者: 娄群峰 E-mail:qflou@njau.edu.cn
  • 基金资助:
    国家“十三五”重点研发计划项目(2016YFD0100204-25);江苏省农业科技自主创新基金项目(CX(20)2019)

Genetic Mapping for a Medium Short-fruit Mutant of Cucumber

CHENG Feng, SONG Mengfei, CAO Lei, ZHANG Mengru, YANG Zhige, CHEN Jinfeng, LOU Qunfeng*()   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2021-03-29 Revised:2021-05-06 Online:2021-07-25 Published:2021-08-10
  • Contact: LOU Qunfeng E-mail:qflou@njau.edu.cn

摘要:

中短果(medium short-fruit)突变体msf黄瓜为种植‘长春密刺’黄瓜自交系的过程中发现的1株自然突变材料,表现为果实长度以及果把长度相对野生型偏短,还表现出植株偏矮,侧枝发育异常,以及主茎脆弱等表型。通过石蜡切片和扫描电镜观察发现突变体果实长度的差异是由于细胞变小引起的。遗传分析发现F2分离群体中野生型表型长果与突变体表型中短果的分离比符合孟德尔3:1遗传定律,表明msf突变性状为单基因控制的隐性性状。BSA-seq(Bulked Segregant Analysis Coupled with Whole-Genome Sequencing)分析将候选基因定位于黄瓜基因组1号染色体26.7 ~ 30.9 Mb区域内。利用437株中短果和‘hazerd’(欧洲温室型短果)杂交的F2群体将基因定位在28.4 ~ 29.8 Mb之间的1.4 Mb区间内。结合dCAPS标记验证将CsaV3_1G044310作为候选基因,该基因编码拟南芥Ⅱ形肌醇多磷酸5-磷酸酶(Ⅱ型5PTase)同源蛋白。通过基因定量表达和内源激素生长素和赤霉素的测定进一步表明该突变体中Ⅱ型肌醇多磷酸5-磷酸酶表达缺陷可能引起激素水平的变化从而影响植株的生长发育。

关键词: 黄瓜, 果实长度, 突变体, 基因定位, Ⅱ型肌醇多磷酸5-磷酸酶;

Abstract:

We isolated a natural mutant,msf(medium short-fruit),from the cucumber inbred line CCMC,a North China type with long fruits. The mutant showed the phenotype of shorter fruit length and fruit neck length than the wild type and also showed short plant,abnormal development of lateral branches and fragile main stem. Through paraffin section and scanning electron microscope observation,it was found that the difference of mutant fruit length was caused by the change of cell size. Genetic analysis showed that the segregation ratio of wild-type phenotype long fruit and mutant phenotype medium short fruit in F2 segregation population conformed to Mendel’s 3︰1 genetic law. This indicated that msf mutation was a recessive trait controlled by a single gene. BSA-seq analysis showed that the candidate gene was located in a 26.7-30.9 Mb region of chromosome 1 of cucumber genome. The msf gene was mapped to a 28.4-29.8 Mb region using a F2 population containing 437 individuals from a cross between msf and ‘hazerd’(a European greenhouse-type inbred line with short fruit). CsaV3_1G044310 was identified as the candidate gene by genome-wide screening combined with dCAPS marker verification,which encodes a homologous protein of Arabidopsis type Ⅱ inositol polyphosphate 5-phosphatase(type Ⅱ 5 Ptase). The quantitative expression of gene and the determination of endogenous hormones auxin and gibberellin the mutant may cause the change of hormone levels and affect the growth and development of plant.

Key words: cucumber, fruit length, mutant, gene mapping, type Ⅱ inositol polyphosphate 5-phosphatase;

中图分类号: