马铃薯低温胁迫相关基因StHY5的功能分析

doi:10.16420/j.issn.0513-353x.2024-0723

园艺学报 ›› 2025, Vol. 52 ›› Issue (7): 1745-1757.doi: 10.16420/j.issn.0513-353x.2024-0723

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

马铃薯低温胁迫相关基因StHY5的功能分析

窦雪婷¹, 朱熙²^,³, 张宁¹^,^*(), 司怀军¹

¹ 甘肃农业大学生命科学技术学院，省部共建干旱生境作物学国家重点实验室，兰州 730070
² 中国热带农业科学院南亚热带作物研究所，农业农村部热带果树生物学重点实验室/海南省热带园艺采后处理与保鲜重点实验室，广东湛江 524091
³ 中国热带农业科学院三亚研究院，热带作物生物育种全国重点实验室，海南三亚 572024

收稿日期:2024-11-12 修回日期:2025-04-22 出版日期:2025-07-23 发布日期:2025-07-23
通讯作者:
*E-mail：ningzh@gsau.edu.cn
基金资助:
甘肃省科技重大专项计划项目(23ZDNA006); 国家重点研发计划项目(2022YFD1602103)

Functional Analysis of StHY5 Associated with Low-Temperature Stress in Potato

DOU Xueting¹, ZHU Xi²^,³, ZHANG Ning¹^,^*(), and SI Huaijun¹

¹ State Key Laboratory of Aridland Crop Science，College of Life Science and Technology，Gansu Agricultural University，Lanzhou 730070，China
² Key Laboratory of Tropical Fruit Biology，Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Tropical Horticulture Postharvest Treatment and Preservation，Institute of South Subtropical Crops，Chinese Academy of Tropical Agricultural Sciences，Zhanjiang，Guangdong 524091，China
³ National Key Laboratory of Biological Breeding of Tropical Crops，Sanya Research Institute，Chinese Academy of Tropical Agricultural Sciences，Sanya，Hainan 572024，China

Received:2024-11-12 Revised:2025-04-22 Published:2025-07-23 Online:2025-07-23

摘要/Abstract

摘要： HY5是属于bZIP类的转录因子，参与调控植物光响应及逆境胁迫。以马铃薯栽培品种‘大西洋’为试验材料，分析StHY5基因及其编码蛋白的功能，并进行亚细胞定位分析。qRT-PCR分析StHY5在4 ℃低温胁迫下的表达模式，构建StHY5的过表达和干扰表达载体，通过遗传转化技术获得马铃薯转基因植株，分析4 ℃低温处理下活性氧相关物质的变化。结果显示：StHY5基因编码区长477 bp，编码了158个氨基酸，定位于细胞核，属于不稳定亲水性蛋白，且无信号肽和跨膜结构，在不同物种中进化较保守，与野生番茄、窄刀薯的亲缘关系较近。StHY5蛋白与10个蛋白存在互作，包括1个光敏色素A，2个B-box蛋白和3个组蛋白脱乙酰酶。StHY5基因启动子区含有光响应元件和激素响应相关元件。qRT-PCR结果表明，低温胁迫可诱导StHY5基因表达量上调。低温处理后，StHY5基因过表达植株的超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性和脯氨酸（Pro）含量较野生型植株显著升高，丙二醛（MDA）含量较野生型植株显著降低；StHY5基因干扰表达植株则反之。

关键词: 马铃薯, StHY5基因, 低温, 生物信息分析, 遗传转化

Abstract:

HY5 is a class of bZIP transcription factor. HY5 is involved in regulating plant light response and abiotic stress. In this study，the functions of the StHY5 and its encoded protein were analyzed，and the subcellular localization was observed using potato cultivar‘Atlantic’as the experimental material. The expression pattern of StHY5 gene was analyzed under 4 ℃ low-temperature stress by qRT-PCR method. The overexpression and interference expression vectors of StHY5 gene were constructed，and obtained transgenic potato plants through genetic transformation technology. The changes of reactive oxygen species under 4 ℃ low-temperature treatment. The results showed that the coding sequence of StHY5 gene was 477 bp，encoding 158 amino acids. StHY5 protein was located in the nucleus，and it belonged to unstable hydrophilic proteins，without signal peptide and transmembrane structure. It had conserved evolutionary among different species，and had the highest evolutionary relationship with wild tomato and Solanum stenotomum. StHY5 protein was closely related to 10 proteins interaction，including 1 photosensitive pigment A，2 B-box proteins，and 3 histone deacetylases. There were cis-acting elements involved in light-responsive and hormone-responsive in the promoter region of StHY5 gene. The qRT-PCR results showed that StHY5 gene was consistently up-regulated in expression during low-temperature. The superoxide dismutase（SOD），peroxidase（POD），catalase（CAT）activities and proline（Pro）content of StHY5 gene overexpression plants were significantly higher and the malondialdehyde（MDA）content was significantly lower than wild type plants under low-temperature treatment. However，StHY5 gene interference expression plants was reversed under low-temperature treatment.

Key words: potato, StHY5, low temperature, bioinformatics analysis, genetic transformation

窦雪婷, 朱熙, 张宁, 司怀军. 马铃薯低温胁迫相关基因StHY5的功能分析[J]. 园艺学报, 2025, 52(7): 1745-1757.

DOU Xueting, ZHU Xi, ZHANG Ning, and SI Huaijun. Functional Analysis of StHY5 Associated with Low-Temperature Stress in Potato[J]. Acta Horticulturae Sinica, 2025, 52(7): 1745-1757.

图/表 11

表1 本研究中PCR和qRT-PCR的特异性引物

Table 1 Specific primers for PCR and qRT-PCR in this study

用途Usage	引物名称Primer name	引物序列Primer sequence（5′-3′）
基因克隆 Gene cloning	StHY5-F	CGGGGGACGAGCTCGGTACCATGCAAGAGCAAGCGACAAGTTC
	StHY5-R	TGCTCACCATGTCGACTTACTTCCTCCCTTCCTTTGCACC
	pMD18-StHY5-F	CGGGGGACGAGCTCGGTACCGGGCCCCCCCTCGAGGTCG
	pMD18-StHY5-R	CCATGTCGACTCTAGAACTAGTGGATCCCCCCATGGC
	StHY5-YF	CGGGGGACGAGCTCGGTACCATGCAAGAGCAAGCGACAAGTTC
	StHY5-YR	TGCTCACCATGTCGACCTTCCTCCCTTCCTTTGCACC
	HYG-F	GCTTCTGCGGGCGATTTGTGT
	HYG-R	GGTCGCGGAGGCTATGGATGC
实时荧光定量PCR qRT-PCR	StHY5-qF	CAGCAAGCAAGGGAGAGGAA
实时荧光定量PCR qRT-PCR	StHY5-qR	TTCCTCCCTTCCTTTGCACC
内参基因 Reference gene	StEf1α-F	GATGGTCAGACCCGTGAACA
内参基因 Reference gene	StEf1α-R	CCTTGGAGTACTTCGGGGTG

表2 amiR-StHY5的PCR扩增引物

Table 2 Primers of amiR-StHY5 for PCR

引物名称Primer name	引物序列（5′-3′）Primer sequence
Ⅰ	gaTTTCAGTATATGTCTAAGCGTtctctcttttgtattcc
Ⅱ	gaACGCTTAGACATATACTGAAAtcaaagagaatcaatga
Ⅲ	gaACACTTAGACATAAACTGAATtcacaggtcgtgatatg
Ⅳ	gaATTCAGTTTATGTCTAAGTGTtctacatatatattcct
A	CTGCAAGGCGATTAAGTTGGGTAAC
B	GCGGATAACAATTTCACACAGGAAACAG

图1 StHY5蛋白二级结构（A）与三级结构（B）

Fig. 1 Secondary structure（A）and tertiary structure（B）of StHY5 protein

图2 StHY5蛋白及其他物种同源蛋白多序列比对

Fig. 2 Multi-sequence alignment of StHY5 protein and other homologous proteins of species

图3 StHY5蛋白系统发育进化树

Fig. 3 StHY5 protein phylogenetic evolutionary tree

图4 StHY5基因扩增产物条带（A）及amiR-StHY5前体片段扩增（B）

Fig. 4 StHY5 gene amplification product band（A）and amiR-StHY5 precursor fragment（B）

图5 pCEGFP-StHY5亚细胞定位

Fig. 5 Subcellular localization of pCEGFP-StHY5

图6 低温处理下StHY5基因的相对表达量误差线表示标准误，不同小写字母表示显著性差异（P < 0.05）

Fig. 6 Relative expression of StHY5 gene under low temperature treatment The error line indicates the standard error，different lowercase letters represent significant differences（P < 0.05）

图7 过表达植株（A）和干扰表达植株（B）的PCR鉴定 +：阳性对照；-：阴性对照

Fig. 7 PCR identification of transgenic plants +：Positive control；-：Negative control

图8 过表达植株（A）和干扰表达植株（B）的qRT-PCR鉴定 WT：野生型。误差线表示标准误，不同小写字母表示显著性差异（P < 0.05），下同

Fig. 8 Relative expression of the transgenic plants by qRT-PCR WT：Wild type. The error line indicates the standard error，different lowercase letters represent significant differences（P < 0.05），the same below

图9 低温胁迫下马铃薯生理指标

Fig. 9 Physiological indexes of the transgenic plants under low temperature stress

参考文献 40

[1]	Ang L H, Chattopadhyay S, Wei N, Oyama T, Okada K, Batschauer A, Deng X W. 1998. Molecular interaction between COP1 and HY 5 defines a regulatory switch for light control of Arabidopsis development. Molecular Cell, 1 (2):213-222. doi: 10.1016/s1097-2765(00)80022-2 pmid: 9659918
[2]	Boyer J S. 1982. Plant productivity and environment. Science, 218 (4571):443-448. doi: 10.1126/science.218.4571.443 pmid: 17808529
[3]	Burman N, Bhatnagar A, Khurana J P. 2018. OsbZIP48,a HY 5 transcription factor ortholog,exerts pleiotropic effects in light-regulated development. Plant Physiology, 176 (2):1262-1285. doi: 10.1104/pp.17.00478 pmid: 28775143
[4]	Catalá R, Medina J, Salinas J. 2011. Integration of low temperature and light signaling during cold acclimation response in Arabidopsis. Proceedings of the National Academy of Sciences, 108 (39):16475-16480.
[5]	Chen X, Yao Q, Gao X, Jiang C, Harberd N P, Fu X. 2016. Shoot-to-root mobile transcription factor HY 5 coordinates plant carbon and nitrogen acquisition. Current Biology, 26 (5):640-646.
[6]	Cluis C P, Mouchel C F, Hardtke C S. 2004. The Arabidopsis transcription factor HY 5 integrates light and hormone signaling pathways. The Plant Journal, 38 (2):332-347.
[7]	Deng Fuyuan, Qiao Zhongquan, Wang Xiaoming, Li Chunxia, Lu Liushu, Li Xuelu, He Gang. 2022. Cloning,subcellular localization and expression analysis of LiHY5 gene in Lagerstroemia indica under different light quality. Journal of Jiangxi Agricultural University, 44 (6):1546-1554. (in Chinese)
	邓涪元, 乔中全, 王晓明, 李春霞, 陆柳淑, 李雪露, 何钢. 2022. 紫薇LiHY5基因的克隆、亚细胞定位与不同光质下的表达分析. 江西农业大学学报, 44 (6):1546-1554.
[8]	Deng Shufang, Liu Qian, Liu Ling, Chen Ou, Wang Wenjun, Zeng Kaifang, Deng Lili. 2024. Cloning of mandarin fruit CcHY5 and its function in fruit coloration. Acta Horticulturae Sinica, 51 (5):939-955. (in Chinese)
	邓淑芳, 刘倩, 刘玲, 陈鸥, 王文军, 曾凯芳, 邓丽莉. 2024. 蜜橘CcHY5的克隆及其对果实转色功能的研究. 园艺学报, 51 (5):939-955.
[9]	Deng Yingyi, Zheng Xu, Xiong Jun, Xu Juan, Qin Weizhi. 2017. Identification of cold resistance of new potato variety Guinongshu No.1 winter seed in the field. Southern Journal of Agriculture, 48 (1):66-71. (in Chinese)
	邓英毅, 郑虚, 熊军, 许娟, 覃维治. 2017. 马铃薯新品种桂农薯1号冬种田间耐寒性鉴定. 南方农业学报, 48 (1):66-71.
[10]	Foley R C, Grossman C, Ellis J G, Llewellyn D J, Dennis E S, Peacock W J, Singh K B. 1993. Isolation of a maize bZIP protein subfamily:candidates for the ocs-element transcription factor. The Plant Journal, 3 (5):669-679.
[11]	Franklin K A, Toledo-Ortiz G, Pyott D E, Halliday K J. 2014. Interaction of light and temperature signaling. Journal of Experimental Botany, 65 (11):2859-2871. doi: 10.1093/jxb/eru059 pmid: 24569036
[12]	Gangappa S N, Botto J F. 2016. The multifaceted roles of HY 5 in plant growth and development. Molecular Plant, 9 (10):1353-1365.
[13]	Kim S, Hwang G, Lee S, Zhu JY, Paik I, Nguyen T T, Kim J, Oh E. 2017. High ambient temperature represses anthocyanin biosynthesis through degradation of HY5. Frontiers in Plant Science,8:1787.
[14]	Koornneef M, Rolff E, Spruit C J P. 1980. Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana(L.) Heynh. Zeitschrift für Pflanzenphysiologie, 100 (2):147-160.
[15]	Li J, Li G, Gao S, Martinez C, He G, Zhou Z, Huang X, Lee J, Zhang H, Shen Y, Wang H, Deng X W. 2010. Arabidopsis transcription factor ELONGATED HYPOCOTYL5 plays a role in the feedback regulation of phytochrome A signaling. The Plant Cell, 22 (11):3634-3649.
[16]	Li Y, Shi Y, Li M, Fu D, Wu S, Li J, Gong Z, Liu H, Yang S. 2021. The CRY2-COP1-HY5-BBX7/ 8 module regulates blue light-dependent cold acclimation in Arabidopsis. The Plant Cell, 33 (11):3555-3573.
[17]	Liu W T, Zhang L C, Ma L, Yuan L, Wang A D. 2023. The HY 5 transcription factor negatively regulates ethylene production by inhibiting ACS1 expression under blue light conditions in pear. Horticultural Plant Journal, 9 (5):920-930.
[18]	Liu X, Wei J, Li S, Li J, Cao H, Huang D, Zhang D, Zhang Z, Gao T, Zhang Y, Ma F, Li C. 2024. MdHY 5 positively regulates cold tolerance in apple by integrating the auxin and abscisic acid pathways. New Phytologist, 246 (5):2155-2173.
[19]	Liu Y, Roof S, Ye Z, Giovannoni J. 2004. Manipulation of light signal transduction as a means of modifying fruit nutritional quality in tomato. Proceedings of the National Academy of Sciences, 101 (26):9897-9902.
[20]	Ma R, Tian N, Wang J, Fan M, Wang B, Qu P, Xu S, Xu Y, Cheng C, Lv P. 2022a. Genome-wide identification and characterization of banana Ca²⁺-ATPase genes and expression analysis under different concentrations of Ca²⁺ treatments. International Journal of Molecular Sciences, 23 (19):11914.
[21]	Ma X, Gai WX, Li Y, Yu YN, Ali M, Gong ZH. 2022b. The CBL-interacting protein kinase CaCIPK13 positively regulates defence mechanisms against cold stress in pepper. Journal of Experimental Botany, 73 (5):1655-1667.
[22]	Nijhawan A, Jain M, Tyagi A K, Khurana J P. 2008. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice. Plant Physiology, 146 (2):333-350. doi: 10.1104/pp.107.112821 pmid: 18065552
[23]	Nishimura R, Ohmori M, Fujita H, Kawaguchi M. 2002. A Lotus basic leucine zipper protein with a RING-finger motif negatively regulates the developmental program of nodulation. Proceedings of the National Academy of Sciences, 99 (23):15206-15210.
[24]	Oyama T, Shimura Y, Okada K. 1997. The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus-induced development of root and hypocotyl l. Genes & Development, 11 (22):2983-2995.
[25]	Petersen H V, Serup P, Leonard J, Michelsen B K, Madsen O D. 1994. Transcriptional regulation of the human insulin gene is dependent on the homeodomain protein STF1/IPF1 acting through the CT boxes. Proceedings of the National Academy of Sciences, 91 (22):10465-10469.
[26]	Si Huaijun, Xie Conghua, Liu Jun. 2003. An efficient protocol for agrobacterium-mediated transformation with microtuber and the introduction of an antisense class I patatin gene into potato. Acta Agronomica Sinica, 29 (6):801-805. (in Chinese)
	司怀军, 谢从华, 柳俊. 2003. 农杆菌介导的马铃薯试管薯遗传转化体系的优化及反义class I patatin基因的导入. 作物学报, 29 (6):801-805.
[27]	Song J, Lin R, Tang M, Wang L, Fan P, Xia X, Yu J, Zhou Y. 2023. SlMPK1-and SlMPK2-mediated SlBBX17 phosphorylation positively regulates CBF-ependent cold tolerance in tomato. New Phytologist, 239 (5):1887-1902.
[28]	Tan Zhengwei, Lu Dandan, Li Lei, Yu Yongliang, Xu Lanjie, Yang Hongqi, Yang Qing, Dong Wei, Li Chunming, An Sufang, Lu Hailing, Liang Huizhen. 2022. Cloning and expression analysis of CtHY5,a key gene of light-regulated signaling pathway in safflower. Chinese Herbal Medicine, 53 (18):5825-5833. (in Chinese)
	谭政委, 鲁丹丹, 李磊, 余永亮, 许兰杰, 杨红旗, 杨青, 董薇, 李春明, 安素妨, 芦海灵, 梁慧珍. 2022. 红花光调控信号途径关键基因CtHY5的克隆及表达分析. 中草药, 53 (18):5825-5833.
[29]	Ulm R, Baumann A, Oravecz A, Nagy F. 2004. Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis. Proceedings of the National Academy of Sciences, 101 (5):1397-1402.
[30]	Wang F, Zhang L, Chen X, Wu X, Xiang X, Zhou J, Xia X, Shi K, Yu J, Foyer C H. 2019. SlHY5integrates temperature,light,and hormone signaling to balance plant growth and cold tolerance. Plant Physiology, 179 (2):749-760.
[31]	Wang Mengyao, Wang Mengjie, Lai Huiping, He Yaping, Yan Lu, Li Peng, Guo Liting, Ai Ye. 2024. Research progress on the effect of temperature on anthocyanin accumulation in plants. Acta Horticulturae Sinica, 51 (7):1501-1515. (in Chinese) doi: 10.16420/j.issn.0513-353x.2023-0556
	王梦瑶, 王梦洁, 赖慧萍, 贺雅萍, 鄢璐, 黎鹏, 郭丽婷, 艾叶. 2024. 温度影响植物花青苷积累的研究进展. 园艺学报, 51 (7):1501-1515.
[32]	Wang Z, Cheng K, Wan L, Yan L, Jiang H, Liu S, Lei Y, Liao B. 2015. Genome-wide analysis of the basic leucine zipper(bZIP)transcription factor gene family in six legume genomes. BMC Genomics,16:1053.
[33]	Weller J L, Hecht V, Vander Schoor J K, Davidson S E, Ross J J. 2009. Light regulation of gibberellin biosynthesis in pea is mediated through the COP1/HY 5 pathway. The Plant Cell, 21 (3):800-813.
[34]	Xu D. 2020. COP1 and BBXs‐HY5‐mediated light signal transduction in plants. New Phytologist, 228 (6):1748-1753.
[35]	Yamawaki S, Yamashino T, Nakanishi H, Mizuno T. 2011. Functional characterization of HY 5 homolog genes involved in early light-signaling in Physcomitrella patens. Bioscience,Biotechnology,and Biochemistry, 75 (8):1533-1539.
[36]	Yang Ying, Gao Shiqing, Tang Yimiao, Ye Xiaofang, Wang Yongbo, Liu Meiying, Zhao Changping. 2009. Research progress on BZIP transcription factors in plants. Journal of Triticeae Crops, 29 (4):730-737. (in Chinese)
	杨颖, 高世庆, 唐益苗, 冶晓芳, 王永波, 刘美英, 赵昌平. 2009. 植物bZIP转录因子的研究进展. 麦类作物学报, 29 (4):730-737.
[37]	Zhang Chao, Zhang Hui, Zhao Xiaoyan, Ma Yue, Yao Huiyuan. 2009. Secondary structure of winter wheat bran antifreeze proteins using circular dichroism spectroscopy and infrared spectroscopy. Spectroscopy and Spectral Analysis, 29 (7):1764-1767. (in Chinese) pmid: 19798935
	张超, 张晖, 赵晓燕, 马越, 姚惠源. 2009. 使用圆二色性光谱和红外光谱研究冬小麦麸皮抗冻蛋白的二级结构. 光谱学与光谱分析, 29 (7):1764-1767. pmid: 19798935
[38]	Zhang L, Jiang X, Liu Q, Ahammed G J, Lin R, Wang L, Shao S, Yu J, Zhou Y. 2020. The HY5 and MYB 15 transcription factors positively regulate cold tolerance in tomato via the CBF pathway. Plant,Cell & Environment, 43 (11):2712-2726.
[39]	Zhang Li, Zhou Bo, Li Yuhua. 2010. Research progress on the structure and function of plant HY 5 protein. Plant Physiol Communications, 46 (10):985-990. (in Chinese)
	张荔, 周波, 李玉花. 2010. 植物HY5蛋白结构与功能的研究进展. 植物生理学通讯, 46 (10):985-990.
[40]	Zhang Y, Liu Z, Liu R, Hao H, Bi Y. 2011. Gibberellins negatively regulate low temperature-induced anthocyanin accumulation in a HY5/HYH-dependent manner. Plant Signaling & Behavior, 6 (5):632-634.

马铃薯低温胁迫相关基因StHY5的功能分析

Functional Analysis of StHY5 Associated with Low-Temperature Stress in Potato

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 40

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

[1]	胡云, 文春淮, 熊兴成, 喻本雨, 何胜华. 耐低温抽薹大白菜新品种‘卓春白1号’[J]. 园艺学报, 2025, 52(S1): 81-82.
[2]	杨利, 覃亚, 边巴次仁, 珍珍, 穷吉, 白玛曲珍, 周雅. 彩色马铃薯新品种‘喜格孜2号’[J]. 园艺学报, 2025, 52(S1): 145-146.
[3]	田红梅, 王元龙, 王飞, 潘弘, 陶珍, 张建, 王朋成. 厚皮甜瓜新品种‘金种缘金甜’[J]. 园艺学报, 2025, 52(S1): 153-154.
[4]	宋倩娜, 宋陆帅, 段永红, 白小东, 冯瑞云. 马铃薯CRISPR/Cas9介导的毛状根高效基因编辑体系的建立[J]. 园艺学报, 2025, 52(7): 1758-1768.
[5]	叶艳然, 刘建刚, 卞春松, 郭华春, 金黎平. 利用RGB影像量化分析不同氮素管理对马铃薯生长的影响[J]. 园艺学报, 2025, 52(7): 1870-1882.
[6]	祝兴喆, 苏仙月, 苏旺, 张红林, 潘哲超, 刘涛, 徐笑宇. 两个马铃薯品种冬、春种植模式下叶片糖苷生物碱合成代谢差异分析[J]. 园艺学报, 2025, 52(7): 1883-1900.
[7]	李姿燕, 陈炜曦, 李子涵, 黎茵, 梁峰铭, 曾祥利, 荐红举, 吕典秋. 基于RNA-Seq筛选调控马铃薯熟性的候选基因[J]. 园艺学报, 2025, 52(6): 1505-1518.
[8]	闫丽娟, 欧承刚, 梁宸, 胡天阔, 刘星, 杨净, 蔺国仓, 庄飞云. 胡萝卜先期抽薹对低温和日照长度的响应[J]. 园艺学报, 2025, 52(6): 1588-1598.
[9]	萧志浩, 郑涵锴, 张曼楠, 唐怀千, 王嘉颖, 张余洋, 张俊红, 叶志彪, 叶杰. 非生物胁迫下钾对番茄苗期生长发育的影响[J]. 园艺学报, 2025, 52(6): 1599-1618.
[10]	王文龙, 刘照坤, 鲁文君, 王耀龙, 李晓锋, 朱红芳, 刘同坤, 李英, 侯喜林, 张昌伟. 利用基因编辑技术提高不结球白菜抗坏血酸的含量[J]. 园艺学报, 2025, 52(5): 1317-1325.
[11]	林茜, 邓振鹏, 阳新月, 周克友, 易小平, 王季春. 减施化肥配施有机肥对马铃薯产量及养分利用的影响[J]. 园艺学报, 2025, 52(4): 1007-1019.
[12]	王中一, 刘熠, 胡博文, 朱凡, 刘峰, 杨莎, 熊程, 欧立军, 戴雄泽, 邹学校. 基于RUBY及CaREF1的辣椒高效遗传转化体系构建[J]. 园艺学报, 2025, 52(4): 1093-1104.
[13]	邢志淦, 雷向朝, 王浩臣, 冯铭鑫, 李靖雯, 刘羽佳, 房玉林, 孟江飞. 不同砧木‘阳光玫瑰’葡萄幼树对盐与低温复合胁迫的生理响应[J]. 园艺学报, 2025, 52(3): 693-704.
[14]	周进华, 白磊, 张锐, 郭华春. 秸秆覆盖降温栽培对马铃薯生长的影响[J]. 园艺学报, 2025, 52(2): 453-466.
[15]	梁国平, 曾宝珍, 刘铭, 边志远, 陈佰鸿, 毛娟. 山葡萄VaSR基因家族的鉴定及VaSR1抗寒功能验证与互作蛋白筛选[J]. 园艺学报, 2025, 52(1): 37-50.

马铃薯低温胁迫相关基因StHY5的功能分析

Functional Analysis of StHY5 Associated with Low-Temperature Stress in Potato

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 40

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: