基于RNA-Seq筛选调控马铃薯熟性的候选基因

doi:10.16420/j.issn.0513-353x.2024-0315

园艺学报 ›› 2025, Vol. 52 ›› Issue (6): 1505-1518.doi: 10.16420/j.issn.0513-353x.2024-0315

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

基于RNA-Seq筛选调控马铃薯熟性的候选基因

李姿燕¹^,², 陈炜曦¹^,², 李子涵¹^,², 黎茵¹^,², 梁峰铭⁵, 曾祥利⁵, 荐红举¹^,²^,³^,⁴^,^*(), 吕典秋¹^,²^,³^,⁴^,^*()

¹ 西南大学，西部（重庆）科学城种质创制大科学中心，重庆 400715
² 西南大学农学与生物科技学院，重庆 400715
³ 西南大学，南方山地农业教育部工程研究中心，重庆 400715
⁴ 西南大学，薯类生物学与遗传育种重庆市重点实验室，重庆 400715
⁵ 巫溪县薯光农业科技发展有限公司，重庆 405899

收稿日期:2025-01-20 修回日期:2025-03-06 出版日期:2025-06-20 发布日期:2025-06-20
通讯作者:
*E-mail：hjjian518@swu.edu.cn；
smallpotatoes@126.com
基金资助:
国家重点研发计划项目(2022YFD1201600); 国家自然科学基金项目(32101659); 重庆市现代农业产业技术体系项目(CQMAITS202303); 西南大学人才引进项目(SWU019008); 中央高校基本科研业务费专项(SWU-KT22036)

Screening Candidate Genes Controlling Potato Maturation Time Based on RNA-Seq

LI Ziyan¹^,², CHEN Weixi¹^,², LI Zihan¹^,², LI Yin¹^,², LIANG Fengming⁵, ZENG Xiangli⁵, JIAN Hongju¹^,²^,³^,⁴^,^*(), and LÜ Dianqiu¹^,²^,³^,⁴^,^*()

¹ Integrative Science Center of Germplasm Creation in Western China（Chongqing）Science City，Southwest University，Chongqing 400715，China
² College of Agronomy and Biotechnology，Southwest University，Chongqing 400715，China
³ Engineering Research Center of South Upland Agriculture，Ministry of Education，Southwest University，Chongqing 400715，China
⁴ Chongqing Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops，Southwest University，Chongqing 400715，China
⁵ Wuxi County Shuguang Agricultural Science and Technology Development Co.，Ltd.，Chongqing 405899，China

Received:2025-01-20 Revised:2025-03-06 Published:2025-06-20 Online:2025-06-20

摘要/Abstract

摘要： 以早熟品种‘早大白’‘中薯5号’‘鄂马铃薯3号’与中晚熟品种‘大西洋’和‘青薯9号’为材料，分析了马铃薯顶端分生组织发育与结薯时间的关系，并利用RNA-Seq技术研究早大白与青薯9号差异表达基因的功能及可能参与的调控通路。结果表明，相比晚熟品种，早熟品种的顶端分生组织向生殖生长转变更早。转录组测序共鉴定出差异表达基因2 842个，其中与激素相关的基因85%集中在激素信号转导途径，特别是生长素（IAA）、脱落酸（ABA）和茉莉酸（JA）等通路；另外，筛选到127个差异表达基因编码转录因子，分布在35个转录因子家族。GO和KEGG富集分析发现差异表达基因主要富集在光合作用、对激素的响应、植物昼夜节律、淀粉和蔗糖代谢、植物激素信号转导等过程。挑选20个可能影响马铃薯结薯早晚的候选基因进行荧光定量PCR验证。其中参与昼夜节律通路的基因FT、HY5，参与淀粉和蔗糖代谢通路的基因BGLU17、CWIN2、GH9B13在早大白中高表达；参与植物激素信号转导或合成代谢的基因GH3、RD22、HB16、CYP74A在早大白中高表达，而IAA17、MES1、RCA在青薯9号中高表达。此外，在早大白中高表达的GRP3可能通过影响生长发育从而调控马铃薯结薯早晚。这些基因可能通过调控光周期响应、激素信号转导、蔗糖水平等在马铃薯中发挥作用，从而影响马铃薯熟性。

关键词: 马铃薯, 转录组测序, 熟性, 顶端分生组织, 差异基因, 光周期, 激素, 蔗糖

Abstract:

In this study，early maturing cultivars‘Zaodabai’‘Zhongshu5’‘Eshu3’，middle late maturing cultivar ‘Atlantic’and late maturing cultivar‘Qingshu 9’were used to analyze the relationship between potato apical meristem development and tuberization time，Zaodabai and Qingshu 9 were also taken to study the function of differentially expressed genes as well as the possible regulatory pathways involved by RNA-Seq. Results showed that the apical meristem turned to reproductive growth earlier in early maturing cultivars than late maturing cultivars. A total of 2 842 differentially expressed genes were identified in transcriptome sequencing，85% of which related to hormones were concentrated in hormone signal transduction pathways and showed rich expression differences，especially in IAA，ABA，JA and other pathways. Besides，a total of 127 differentially expressed transcription factor genes were identified，distributed in 35 transcription factor families. GO and KEGG enrichment analysis showed that differentially expressed genes were mainly concentrated in photosynthesis，hormone response，plant circadian rhythm，starch and sucrose metabolism，and plant hormone signal transduction. The 20 candidate genes that may affect the early and late tuberization of potato were selected for qRT-PCR verification. The genes involved in the circadian rhythm pathway，FT and HY5，were highly expressed in Zaodabai. Genes involved in the starch and sucrose metabolic pathway，BGLU17，CWIN2 and GH9B13 were highly expressed in Zaodabai. GH3，RD22，HB16 and CYP74A involved in plant hormone signal transduction or anabolism were highly expressed in Zaodabai，while IAA17，MES1 and RCA were highly expressed in Qingshu 9. In addition，GRP3 which highly expressed in Zaodabai may also affect tuberization time. These genes may play a role in potato by regulating photoperiodic response，hormone signal transduction，sucrose level，etc.，thus affecting potato tuberization time.

Key words: potato, transcriptome sequencing, maturation, apical meristem, differential expressed gene, photoperiod, hormone, sucrose

李姿燕, 陈炜曦, 李子涵, 黎茵, 梁峰铭, 曾祥利, 荐红举, 吕典秋. 基于RNA-Seq筛选调控马铃薯熟性的候选基因[J]. 园艺学报, 2025, 52(6): 1505-1518.

LI Ziyan, CHEN Weixi, LI Zihan, LI Yin, LIANG Fengming, ZENG Xiangli, JIAN Hongju, and LÜ Dianqiu. Screening Candidate Genes Controlling Potato Maturation Time Based on RNA-Seq[J]. Acta Horticulturae Sinica, 2025, 52(6): 1505-1518.

图/表 8

表1 候选基因qRT-PCR引物序列

Table 1 qRT-PCR primer sequences of candidate genes

名称Name	基因ID Gene ID	正向引物（5′-3′）Forward primer	反向引物（3′-5′）Reverse primer
GH3	Soltu.DM.10G002010	TGAAGCCATTCTCTGCCTAGACTC	AGCCAACACGAACGACTTCTCTG
SAUR79	Soltu.DM.08G022860	GGTAGCAATCTTGACAAGGCGATG	TTCCTTTCCCACCAGCACAGTTAC
IAA17	Soltu.DM.03G035030	GATGGAGACTGGATGCTTGTTGGA	CCTCCGTGCTCTTCATCAACCTAA
RD22	Soltu.DM.08G017220	GGTGTCTATGGTGGGTGCTGATG	ATAGGCTTGTCTCCAGGCTTAACG
HB16	Soltu.DM.11G003380	GGTTGGTTGTTCATCTCCGTTGA	TGTTGTTGTTGTTGTTGACGAAGG
CYP74A	Soltu.DM.10G003720	TGGATTGGAACATCAGGACCTAGT	ACCACAGACTTAACAAGAGGCATT
MES1	Soltu.DM.02G008550	CCTGAAGAGCCTCGGACATCCA	GGTCTCACCAACGACGATGCTAAT
MES3	Soltu.DM.01G048330	TGCCAATCCTCCAACAACCTTCAT	GTAGTAGCCAGTGCCCAGTCCT
RCA	Soltu.DM.10G023180	ACTCACCATCCTGTCTTGTCATCA	ATCCATCCAGCGTGTTGTCCAG
GIM2	Soltu.DM.08G001760	ACTTGGTATGCCACCTCATTCAGA	TCGAGATGGTCGCCGGTATTG
FT	Soltu.DM.05G026370	CAAGCCCAAGCGACCCTAACTT	CGTAGGTGTTGGATTCTCGTAGCA
COP1	Soltu.DM.11G001010	AGGAGGAAGAGGAAGAGGAAGAGG	AACTATGTCCACACGCCGTTAGAA
HY5	Soltu.DM.08G011730	TGCCGCTAGTTCACTACCTTCAAG	ACATCGTTCCCGTCGCTTCTC
GRP3	Soltu.DM.09G030440	ATTATGGAGGCGGCTATGGTAAGG	AGTCTGGGCAGCCACATATTCTTC
RBCS3B	Soltu.DM.02G025840	AGGTCTTGGCTGAGGTGGAGGA	AACTGATGCACTGCACTTGACGAA
GH9B13	Soltu.DM.09G023670	ACGAGCGATCAGATTACGAGCAAT	TGAGCAAGGTAAGTGAGTGTTCCA
BGLU45	Soltu.DM.07G024580	ACAAGGTGGAACGATAGGAATGGT	AGGATCAAGAAGCCAGGCAACAT
ADG2	Soltu.DM.01G024440	GCAGGTGAGGCTAAGTTGAAGGAT	CCCATTGGAACGGCAGGCTTA
BGLU17	Soltu.DM.10G010830	GTCGGCTCCCAAGATTCACAGTT	CAGCATGATTGTCAGTGGTGTAGC
CWIN2	Soltu.DM.10G025030	ATGTTGGTTGGGTCAGGATGGTT	CCGTCAACTGAATGAAGTGGATGT

图1 不同马铃薯品种在长日照（A、B）和短日照（C、D）条件下微型薯结薯时间和数量统计早熟：早大白、中薯5号、鄂马铃薯3号；中晚熟：大西洋；晚熟：青薯9号。使用最小显著差异法对数据进行多重比较分析，不同小写字母表示差异显著，（P < 0.05）

Fig. 1 Statistics of tuberization time and number of microtuber in different potato cultivars under long day(A，B)and short day(C，D)condition Early maturing：Zaodabai，Zhongshu5，Eshu3；middle late maturing：Atlantic；late maturing：Qingshu9. The minimum significant difference method was used for multiple comparative analysis of the data. Different lowercase letters indicate significant differences at 0.05 level

图2 长日照条件下马铃薯不同品种组培苗茎顶端分生组织发育绿色箭头代表顶端分生组织进行营养生长，红色箭头代表顶端分生组织已转向生殖生长

Fig. 2 Stem apical meristem development of plantlet of various potato cultivars under long day condition The green arrows indicates that the apical meristem has undergone vegetative growth，the red arrow indicates that the apical meristem has shifted to reproductive growth

图3 马铃薯品种早大白与青薯9号差异基因表达模式聚类分析（A）和表达量差异统计（B）

Fig. 3 Cluster analysis of differential gene expression pattern（A）and statistical difference of expression level（B） between potato cultivars Zaodabai and Qingshu 9

图4 马铃薯品种早大白和青薯9号中植物激素相关基因的表达

Fig. 4 Expression of hormone-related genes in potato cultivars Zaodabai and Qingshu 9

图5 马铃薯品种早大白和青薯9号的差异表达基因中各转录因子家族基因分布

Fig. 5 Distribution of transcription factor family genes in DEGs of potato cultivars Zaodabai and Qingshu 9

图6 马铃薯品种早大白与青薯9号差异表达基因GO（A）和KEGG（B）富集分析

Fig. 6 GO(A)and KEGG(B)enrichment analysis of differentially expressed genes of potato cultivars Zaodabai and Qingshu 9

图7 马铃薯熟性相关候选基因表达模式分析使用t检验对数据进行分析，*代表显著性水平P ≤ 0.05；**代表显著性水平P ≤ 0.01；ns代表无显著性

Fig. 7 Analysis of candidate gene expression patterns related to potato maturation time t-Test was used to analyze the data，* represents a significance level of P ≤ 0.05，** represents a significance level of P ≤ 0.01，ns represents no significance

参考文献 45

[1]	Abelenda J A, Cruz-Oró E, Franco-Zorrilla J M, Prat S. 2016. Potato StCONSTANS-like 1 suppresses storage organ formation by directly activating the FT-like StSP5G repressor. Current Biology, 26 (7):872-881.
[2]	Adavi Z, Moradi R, Saeidnejad A H, Tadayon M R, Mansouri H. 2018. Assessment of potato response to climate change and adaptation strategies. Sci.Hortic,228:91-102.
[3]	Bachem C W, van der Hoeven R S, de Bruijn S M, Vreugdenhil D, Zabeau M, Visser R G. 1996. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP:analysis of gene expression during potato tuber development. Plant J, 9 (5):745-753.
[4]	Begum S, Jing S, Yu L, Sun X, Wang E, Abu Kawochar M, Qin J, Liu J, Song B. 2022. Modulation of JA signaling reveals the influence of StJAZ1-like on tuber initiation and tuber bulking in potato. Plant J, 109 (4):952-964.
[5]	Chailakhyan M K, Yanina L, Devedzhyan A, Lotova G. 1981. Photoperiodism and tuber formation in graftings of tobacco onto potato. Dokl. Akas. Nauk SSSR,257:1276-1280.
[6]	Chincinska I A, Liesche J, Krügel U, Michalska J, Geigenberger P, Grimm B, Kühn C. 2008. Sucrose transporter StSUT4 from potato affects flowering,tuberization,and shade avoidance response. Plant Physiol, 146 (2):515-528.
[7]	Chu L, Yang C, Zhuang F, Gao Y, Luo M. 2022. The HDA9-HY 5 module epigenetically regulates flowering time in Arabidopsis thaliana. Journal of Cellular Physiology, 237 (7):2961-2968.
[8]	Cong Bin, Jia Hongwu, Li Yan, Zhang Pifang, Sun Chongrong. 1999. Studies on morphogenesis and shoot apical meristem(SAM)of rudimentary panicle in rice. Journal of Southern Agriculture, 19 (3):415-421. (in Chinese)
	丛斌, 贾红武, 李严, 张丕方, 孙崇荣. 1999. 水稻幼穗形态发生与顶端分生组织的研究. 西北植物学报, 19 (3):415-421.
[9]	Ewing E E, Struik P C. 1992. Tuber formation in potato:induction,initiation,and growth. Horticultural Reviews,14:89-198.
[10]	Feng Jianying, Li Liqin, Lu Liming. 2022. Genome-wide identification and expression analysis of the bHLH transcription factor family in Solanum tuberosum. Biotechnology Bulletin, 38 (2):21-33. (in Chinese)
	冯建英, 李立芹, 鲁黎明. 2022. 马铃薯bHLH转录因子家族全基因组鉴定与表达分析. 生物技术通报, 38 (2):21-33.
[11]	Fritzius T, Aeschbacher R, Wiemken A, Wingler A. 2001. Induction of ApL3 expression by trehalose complements the starch-deficient Arabidopsis mutant adg2-1 lacking ApL1,the large subunit of ADP-glucose pyrophosphorylase. Plant Physiol, 126 (2):883-889.
[12]	González Schain N D, Díaz Mendoza M, Zurczak M, Suárez López P. 2012. Potato CONSTANS is involved in photoperiodic tuberization in a graft-transmissible manner. Plant J,70:678-690.
[13]	Hannapel D J. 2010. A model system of development regulated by the long-distance transport of mRNA. Plant Biol, 52 (1):40-52.
[14]	Hannapel D J, Sharma P, Lin T, Banerjee A K. 2017. The multiple signals that control tuber formation. Plant Physiol,174:845-856.
[15]	He Puming, Di Shufei. 2019. Research on the development path of potato as staple food under the direction of ecological security and food security. Agricultural Economy,(6):12-14. (in Chinese)
	何蒲明, 狄书非. 2019. 生态安全与粮食安全并重导向下马铃薯主粮化发展路径研究. 农业经济,(6):12-14.
[16]	Hu Yueqing. 2019. Effects of GA3 and ABA on potato shape. Jiangsu Agricultural Sciences, 47 (18):125-128. (in Chinese)
	胡月清. 2019. GA3和ABA对马铃薯薯形的影响. 江苏农业科学, 47 (18):125-128.
[17]	Iranbakhsh A, Ebadi M, Khaniki G B. 2007. The ontogenetic trends of microtuber formation in potato(Solanum tuberosum L.). Pak J Biol Sci, 10 (6):843-851.
[18]	Jez J M. 2022. Connecting primary and specialized metabolism:amino acid conjugation of phytohormones by GRETCHEN HAGEN 3(GH3) acyl acid amido synthetases. Plant Biol,66:102194.
[19]	Kloosterman B, Navarro C, Bijsterbosch G, Lange T, Prat S, Visser R G, Bachem C W. 2007. StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development. Plant J, 52 (2):362-373.
[20]	Kolachevskaya O O, Alekseeva V V, Sergeeva L I, Rukavtsova E B, Getman I A, Vreugdenhil D, Buryanov Y I, Romanov G A. 2015. Expression of auxin synthesis gene tms1 under control of tuber-specific promoter enhances potato tuberization in vitro. Plant Biol, 57 (9):734-744.
[21]	Kolachevskaya O O, Lomin S N, Arkhipov D V, Romanov G A. 2019. Auxins in potato:molecular aspects and emerging roles in tuber formation and stress resistance. The Plant Cell, 38 (6):681-698.
[22]	Kondhare K R, Kumar A, Patil N S, Malankar N N, Saha K, Banerjee A K. 2021. Development of aerial and belowground tubers in potato is governed by photoperiod and epigenetic mechanism. Plant Physiol, 187 (3):1071-1086.
[23]	Kondhare K R, Natarajan B, Banerjee A K. 2020. Molecular signals that govern tuber development in potato. Int J Dev Biol,64:133-140.
[24]	Lebedeva M, Komakhin R, Konovalova L, Ivanova L, Taranov V, Monakhova Y, Babakov A, Klepikova A, Zlobin N. 2022. Development of potato(Solanum tuberosum L.) plants with StLEAFY knockout. Planta, 256 (6):116.
[25]	Li H, Hagen G, Guilfoyle T J. 2023. Do some IAA proteins have two repression domains? Plant Signal Behav, 6 (6):858-860.
[26]	Li X, Liu G, Geng Y, Wu M, Pei W, Zhai H, Zang X, Li X, Zhang J, Yu S, Yu J. 2011. A genome-wide analysis of the small auxin-up RNA(SAUR)gene family in cotton. BMC Genomics, 18 (1):815.
[27]	Li Xiaomei. 2008. Manufaction of paraffin section on apical meristem of soybean. Soybean Science, 27 (4):708-710. (in Chinese)
	李晓梅. 2008. 大豆茎顶端分生组织石蜡切片的制备. 大豆科学, 27 (4):708-710.
[28]	Lifschitz E, Eviatar T, Rozman A, Shalit A, Goldshmidt A, Amsellem Z, Alvarez J P, Eshed Y. 2006. The tomato FT ortholog triggers systemic signals that regulate growth and flowering and substitute for diverse environmental stimuli. Proc Natl Acad Sci USA,103:6398-6403.
[29]	Liu Dan, Xu Mengwei, Hu Yueqing, Wang Ruozhong, Tong Jianhua, Xiao Langtao. 2019. Dynamic changes of key plant hormones during potato tuber development. Molecular Plant Breeding, 17 (6):1998-2003. (in Chinese)
	刘丹, 徐梦薇, 胡月清, 王若仲, 童建华, 肖浪涛. 2019. 马铃薯块茎发育期主要植物激素的动态变化. 分子植物育种, 17 (6):1998-2003.
[30]	Martinez Garcia J F, Virgos Soler A, Prat S. 2002. Control of photoperiod-regulated tuberization in potato by the Arabidopsis flowering-time gene CONSTANS. Proc Natl Acad Sci USA,99:15211-15216.
[31]	Mitra S, Baldwin I T. 2014. RuBPCase activase(RCA)mediates growth-defense trade-offs:silencing RCA redirects jasmonic acid(JA)flux from JA-isoleucine to methyl jasmonate(MeJA)to attenuate induced defense responses in Nicotiana attenuata. New Phytol, 201 (4):1385-1395.
[32]	Rodríguez Falcón M, Bou J, Prat S. 2006. Seasonal control of tuberization in potato:conserved elements with the flowering response. Annu Rev Plant Biol,57:151-180.
[33]	Shan J, Song W, Zhou J, Wang X, Xie C, Gao X, Xie T, Liu J. 2013. Transcriptome analysis reveals novel genes potentially involved in photoperiodic tuberization in potato. Genomics, 102 (4):388-396.
[34]	Shan Jianwei, Liu Jun, Suo Haicui, Wang Li, An Kang, Liu Jitao, Jing Shenglin, Li Chengchen, Song Botao, Li Xiaobo. 2021. Research progress on sugar signaling regulation of potato tuber development. Journal of Huazhong Agricultural University, 40 (4):27-35. (in Chinese)
	单建伟, 柳俊, 索海翠, 王丽, 安康, 刘计涛, 景晟林, 李成晨, 宋波涛, 李小波. 2021. 糖信号调控马铃薯块茎发育的研究进展. 华中农业大学学报, 40 (4):27-35.
[35]	Sun Lei, Tai Feng, Zhang Liang, Yu Hongtao, Su Hang, Bi Shiting. 2018. Effect of different nitrogen fertilizer combinations on formation and development of potato tuber. Journal of Dongbei Agricultural University, 49 (6):32-39. (in Chinese)
	孙磊, 邰枫, 张亮, 于洪涛, 苏航, 毕诗婷. 2018. 不同氮肥配施对马铃薯块茎形成及发育的影响. 东北农业大学学报, 49 (6):32-39.
[36]	Sun W, Ma Z, Chen H, Liu M. 2019. MYB gene family in potato(Solanum tuberosum L.):genome-wide identification of hormone-responsive reveals their potential functions in growth and development. Int J Mol Sci, 20 (19):4847.
[37]	Villányi V, Gondor O K, Bánfalvi Z. 2022. Metabolite profiling of tubers of an early and a late maturing potato line and their grafts. Metabolomics, 18 (11):88.
[38]	Viola R, Roberts A G, Haupt S, Gazzani S, Hancock R D, Marmiroli N, Machray G C, Oparka K J. 2001. Tuberization in potato involves a switch from apoplastic to symplastic phloem unloading. The Plant Cell, 13 (2):385-398.
[39]	Wang Dongxue. 2013. Study on related characters and diversity analysis of potato early tuberization[M. D. Dissertation]. Heilongjiang: Northeast Agricultural University. (in Chinese)
	王冬雪. 2013. 马铃薯早熟相关性状研究及多样性分析[硕士论文]. 黑龙江: 东北农业大学.
[40]	Xie Tingting, Liu Jun. 2013. Molecular mechanism underlying photoperiodic-induced potato tuber formation. Agricultural Sciences in China, 46 (22):4657-4664. (in Chinese)
	谢婷婷, 柳俊. 2013. 光周期诱导马铃薯块茎形成的分子机理研究进展. 中国农业科学, 46 (22):4657-4664.
[41]	Xu D, Zhu D, Deng X W. 2016. The role of COP1 in repression of photoperiodic flowering. F1000Research,doi:10.1146/annurev.arplant.57.032905.105224.
[42]	Xu Qingfen. 2005. Evaluation and separation trend of important agronomic traits of potato early maturing combination[M. D. Dissertation]. Heilongjiang: Northeast Agricultural University. (in Chinese)
	许庆芬. 2005. 马铃薯早熟组合重要农艺性状的评价及分离趋势的研究[硕士论文]. 黑龙江: 东北农业大学.
[43]	Xu Zhen, Xu Chan, Guo Deping. 2008. Molecular mechanism of photoperiod regulation of potato tuber formation. Chinese Journal of Cell Biology,3:731-736. (in Chinese)
	许真, 徐蝉, 郭得平. 2008. 光周期调节马铃薯块茎形成的分子机制. 细胞生物学杂志,3:731-736.
[44]	Yao Xiangyu, Li Guangcun, Xu Jianfei, Bian Chunsong, Jin Liping, Xu Zhigang. 2023. Effects of light on early growth and tuber formation of aeroponic potatoes. Journal of Nanjing Agricultural University, 46 (1):14-22. (in Chinese)
	姚翔宇, 李广存, 徐建飞, 卞春松, 金黎平, 徐志刚. 2023. 光照对雾培马铃薯前期生长及块茎形成的影响. 南京农业大学学报, 46 (1):14-22.
[45]	Zhang Shuo. 2021. Research on potato planting regionalization in China[M. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	张烁. 2021. 中国马铃薯种植区划研究[硕士论文]. 北京: 中国农业科学院.

基于RNA-Seq筛选调控马铃薯熟性的候选基因

Screening Candidate Genes Controlling Potato Maturation Time Based on RNA-Seq

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 45

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

[1]	杨利, 覃亚, 边巴次仁, 珍珍, 穷吉, 白玛曲珍, 周雅. 彩色马铃薯新品种‘喜格孜2号’[J]. 园艺学报, 2025, 52(S1): 145-146.
[2]	杨春梅, 于洋, 丁雨格, 夏京, 周玲, 彭磊. 转录—代谢联合分析‘贵妃’杧果腋芽转化花芽中的淀粉与蔗糖代谢途径[J]. 园艺学报, 2025, 52(6): 1412-1426.
[3]	唐伶俐, 贺玉花, 王庆涛, 安璐璐, 徐永阳, 张健, 孔维虎, 户克云, 赵光伟. 非呼吸跃变和呼吸跃变型甜瓜成熟果实的激素与转录组分析[J]. 园艺学报, 2025, 52(4): 883-896.
[4]	邵一帆, 朱宝庆, 王童欣, 廖建和, 吴繁花, 杨思怡, 冯建行, 于旭东. 基于激素、转录组和代谢组研究木棉皮刺的遗传调控[J]. 园艺学报, 2025, 52(4): 933-946.
[5]	田玉凤, 马松亚, 杨安, 韩晓蕾, 张彩霞. 苹果砧木B9再生体系的建立与优化[J]. 园艺学报, 2025, 52(4): 947-958.
[6]	林茜, 邓振鹏, 阳新月, 周克友, 易小平, 王季春. 减施化肥配施有机肥对马铃薯产量及养分利用的影响[J]. 园艺学报, 2025, 52(4): 1007-1019.
[7]	周进华, 白磊, 张锐, 郭华春. 秸秆覆盖降温栽培对马铃薯生长的影响[J]. 园艺学报, 2025, 52(2): 453-466.
[8]	杨艳, 刘军, 周晓慧, 刘松瑜, 庄勇. 茄子SmWRKY4的克隆及耐冷性功能验证[J]. 园艺学报, 2025, 52(1): 101-110.
[9]	黄勋, 刘霞, 邓琳梅, 王兴国, 徐亚锦, 杨艳丽. 马铃薯疮痂病生防菌1X1Y的鉴定及其生防促生特性研究[J]. 园艺学报, 2025, 52(1): 229-246.
[10]	仲阳, 秦亚芝, 罗帅, 荆玉玲, 王万兴, 李广存, 胡新喜, 秦玉芝, 程旭, 熊兴耀. 泥炭和蘑菇渣改土对马铃薯根源微生物组及产量的影响[J]. 园艺学报, 2024, 51(9): 2143-2154.
[11]	郭群艳, 刘彩贤, 余秋岫, 张画, 郑美婷. 紫花含笑花发育过程中生理和内源激素的变化[J]. 园艺学报, 2024, 51(8): 1881-1890.
[12]	王建昭, 高园, 刀梅, 张洪烨, 杨自云, 陈龙清, 吴田. 山茶叶片的离体再生及内源激素与不定芽分化的关系[J]. 园艺学报, 2024, 51(8): 1891-1905.
[13]	文宋琴, 李佳霖, 池卓恒, 夏燕, 王淑明, 吴頔, 郝雅雯, 郭启高, 梁国鲁, 景丹龙. 光和温度对开花时间基因可变剪接的影响研究进展[J]. 园艺学报, 2024, 51(8): 1949-1963.
[14]	王稳, 张鹏, 王志敏, 刘根忠, 包志龙, 马方放. 菊花脑管状花响应蔗糖饲喂的差异基因分析[J]. 园艺学报, 2024, 51(6): 1297-1310.
[15]	范国权, 喻江, 高艳玲, 李庆全, 张抒, 于镇华. 马铃薯种薯催芽、切块和包衣对其立枯丝核菌病的影响[J]. 园艺学报, 2024, 51(5): 1151-1161.

基于RNA-Seq筛选调控马铃薯熟性的候选基因

Screening Candidate Genes Controlling Potato Maturation Time Based on RNA-Seq

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 45

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: