艾草CBF基因家族的鉴定及表达分析

doi:10.16420/j.issn.0513-353x.2024-0801

摘要/Abstract

摘要：

CBF（C-repeat binding factor）基因家族属于转录激活因子，在响应低温等非生物胁迫中发挥重要作用。为挖掘参与艾草（Artemisia argyi）温度响应的CBF基因家族成员，本研究中通过生物信息方法鉴定出17个艾草AaCBF基因并对其进行分析。结果表明，其编码蛋白氨基酸长度为131 ~ 222 aa，平均等电点为6.08。AaCBF蛋白多数定位在细胞核上。16个基因不均匀分布在5条染色体上，8号和16号染色体分布最多，AaCBF08未定位在染色体上。系统进化树分析该基因家族可分为4个亚族，其中3个亚族中有艾草基因家族成员的分布。所有AaCBF基因家族成员均包含Motif 1，Motif 5和Motif 7，且均含有保守结构域，表现出高度保守性。此外，17个基因均不含有内含子。qRT-PCR结果表明6个候选AaCBF基因在艾草不同组织中表达存在显著差异。高温处理下，4个候选AaCBF的表达量逐渐上升且在12 h时表达量最高，AaCBF04和AaCBF16表达量先上升后下降，12 h时又上升且表达量最高。低温处理下，5个候选AaCBF的表达量先上升后下降且在3 h表达量最高，AaCBF15在12 h时又上升且表达量最高。

关键词: 艾草, CBF基因家族, 生物信息学, 表达分析

Abstract:

CBF（C-repeat binding factor）gene family is categorized as the transcriptional activators and exerts an important role in response to abiotic stresses such as low temperature. In order to investigate the CBF gene family members involved in temperature response of Artemisia argyi，17 AaCBF genes of A. argyi were identified and analyzed by bioinformatics in this study. It was found that the amino acid length of the encoded protein ranged from 131-222 aa，with an average isoelectric point of 6.08. Most of the AaCBF proteins were localized in the nucleus. The 16 genes were unevenly distributed on 5 chromosomes，with the highest distribution on chromosomes 8 and 16. AaCBF08 was not localized to chromosomes. Based on the phylogenetic tree analysis，the gene family could be divided into 4 subfamilies，with members of the A. argyi gene family distributed across 3 of these subfamilies. All AaCBF gene family members contained Motif 1，Motif 5 and Motif 7，and conserved domains，demonstrating a high level of conservation. In addition，none of 17 genes contained introns. qRT-PCR results showed that six candidate AaCBF genes were significantly differentially expressed across different tissues of A. argyi. Under high-temperature treatment，the expression levels of the four candidate AaCBF genes gradually increased and reached the highest expression at 12 hours. Notably，the expression levels of AaCBF04 and AaCBF16 initially increased，subsequently decreased，and increased again at 12 hours and reached its highest level. Under low-temperature treatment，the expression levels of five candidate AaCBF genes first increased and then decreased，with the highest expression at three hours. In contrast，AaCBF15 increased again at 12 hours and reached its highest level.

Key words: Artemisia argyi, CBF gene family, bioinformatics, expression analysis

柳璐, 王梦, 郑晓美, 王珂青, 苗如意, 臧巧路. 艾草CBF基因家族的鉴定及表达分析[J]. 园艺学报, 2025, 52(10): 2703-2712.

LIU Lu, WANG Meng, ZHENG Xiaomei, WANG Keqing, MIAO Ruyi, ZANG Qiaolu. Identification and Expression Analysis of CBF Gene Family of Artemisia argyi[J]. Acta Horticulturae Sinica, 2025, 52(10): 2703-2712.

图/表 7

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基因名称 Gene name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
Aaactin	GTCACACTGGTGTCATGGTTGGAAT	CGTGCTCAATCGGGTACTTCAAAGT
AaCBF02	TAAGAACCTACCGAAGCG	ACCCATTTAGACCCGTTT
AaCBF03	GGGACATACCCAACTGCT	CACTCACCTCTTCCACCA
AaCBF04	GGATGGTGGTGGCTCAAA	CTGGCATACCAAACTCCTCTTC
AaCBF07	ATGGGTTTGTGAAGTGAGA	CATTAGACTCTGGGACTGG
AaCBF15	TGGCGGTGGTAGTTCAGA	TTTCAGCCGTTGGATGTG
AaCBF16	CGAGGAAATTATGTTGGC	GAAGCTGGACTAGGAAGGT

基因名称 Gene name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
Aaactin	GTCACACTGGTGTCATGGTTGGAAT	CGTGCTCAATCGGGTACTTCAAAGT
AaCBF02	TAAGAACCTACCGAAGCG	ACCCATTTAGACCCGTTT
AaCBF03	GGGACATACCCAACTGCT	CACTCACCTCTTCCACCA
AaCBF04	GGATGGTGGTGGCTCAAA	CTGGCATACCAAACTCCTCTTC
AaCBF07	ATGGGTTTGTGAAGTGAGA	CATTAGACTCTGGGACTGG
AaCBF15	TGGCGGTGGTAGTTCAGA	TTTCAGCCGTTGGATGTG
AaCBF16	CGAGGAAATTATGTTGGC	GAAGCTGGACTAGGAAGGT

基因ID Gene ID	基因名 Gene name	氨基酸数 Amino acid number	分子量/kD Molecular weight	理论等电点 pI	不稳定指数 Instability index	脂溶性指数 Lipid solubility index	亲水平均数 Grand average of hydropathicity	亚细胞定位 Subcellular location
Aarg03G000760.1	AaCBF01	212	23.69	6.45	52.28	61.70	-0.46	细胞核Nucleus
Aarg08G006190.1	AaCBF02	213	23.97	9.18	55.71	67.84	-0.68	细胞核Nucleus
Aarg08G013720.1	AaCBF03	220	24.43	5.21	47.80	59.91	-0.59	细胞核Nucleus
Aarg08G021730.1	AaCBF04	210	23.14	4.78	63.83	55.33	-0.66	细胞核Nucleus
Aarg08G021740.1	AaCBF05	213	23.94	4.81	61.90	70.89	-0.40	细胞核Nucleus
Aarg08G021760.1	AaCBF06	203	22.53	5.08	51.60	64.43	-0.57	细胞核Nucleus
Aarg08G021780.1	AaCBF07	215	23.96	4.81	47.79	55.91	-0.68	细胞核Nucleus
Aarg0G037100.1	AaCBF08	131	14.70	10.46	51.02	90.15	-0.33	叶绿体 Chloroplast
Aarg10G044550.1	AaCBF09	192	21.73	6.22	54.32	81.30	-0.32	细胞骨架 Cytoskeleton
Aarg10G044900.1	AaCBF10	192	21.74	6.96	56.65	78.75	-0.35	细胞质 Cytoplasmic
Aarg11G000870.1	AaCBF11	212	23.71	6.16	48.30	59.39	-0.49	细胞核Nucleus
Aarg16G007180.1	AaCBF12	207	23.32	8.83	53.19	66.04	-0.68	细胞核Nucleus
Aarg16G013620.1	AaCBF13	222	24.66	5.19	46.22	57.61	-0.59	细胞核Nucleus
Aarg16G021830.1	AaCBF14	215	23.95	4.81	47.39	55.91	-0.67	细胞核Nucleus
Aarg16G021850.1	AaCBF15	203	22.53	5.08	52.54	64.43	-0.57	细胞核Nucleus
Aarg16G022000.1	AaCBF16	210	23.14	4.78	64.39	54.43	-0.64	细胞核Nucleus
Aarg16G022010.1	AaCBF17	220	24.79	4.62	61.29	73.95	-0.42	细胞核Nucleus

基因ID Gene ID	基因名 Gene name	氨基酸数 Amino acid number	分子量/kD Molecular weight	理论等电点 pI	不稳定指数 Instability index	脂溶性指数 Lipid solubility index	亲水平均数 Grand average of hydropathicity	亚细胞定位 Subcellular location
Aarg03G000760.1	AaCBF01	212	23.69	6.45	52.28	61.70	-0.46	细胞核Nucleus
Aarg08G006190.1	AaCBF02	213	23.97	9.18	55.71	67.84	-0.68	细胞核Nucleus
Aarg08G013720.1	AaCBF03	220	24.43	5.21	47.80	59.91	-0.59	细胞核Nucleus
Aarg08G021730.1	AaCBF04	210	23.14	4.78	63.83	55.33	-0.66	细胞核Nucleus
Aarg08G021740.1	AaCBF05	213	23.94	4.81	61.90	70.89	-0.40	细胞核Nucleus
Aarg08G021760.1	AaCBF06	203	22.53	5.08	51.60	64.43	-0.57	细胞核Nucleus
Aarg08G021780.1	AaCBF07	215	23.96	4.81	47.79	55.91	-0.68	细胞核Nucleus
Aarg0G037100.1	AaCBF08	131	14.70	10.46	51.02	90.15	-0.33	叶绿体 Chloroplast
Aarg10G044550.1	AaCBF09	192	21.73	6.22	54.32	81.30	-0.32	细胞骨架 Cytoskeleton
Aarg10G044900.1	AaCBF10	192	21.74	6.96	56.65	78.75	-0.35	细胞质 Cytoplasmic
Aarg11G000870.1	AaCBF11	212	23.71	6.16	48.30	59.39	-0.49	细胞核Nucleus
Aarg16G007180.1	AaCBF12	207	23.32	8.83	53.19	66.04	-0.68	细胞核Nucleus
Aarg16G013620.1	AaCBF13	222	24.66	5.19	46.22	57.61	-0.59	细胞核Nucleus
Aarg16G021830.1	AaCBF14	215	23.95	4.81	47.39	55.91	-0.67	细胞核Nucleus
Aarg16G021850.1	AaCBF15	203	22.53	5.08	52.54	64.43	-0.57	细胞核Nucleus
Aarg16G022000.1	AaCBF16	210	23.14	4.78	64.39	54.43	-0.64	细胞核Nucleus
Aarg16G022010.1	AaCBF17	220	24.79	4.62	61.29	73.95	-0.42	细胞核Nucleus