烟草过表达刺槐RpACBP3对光合生理的影响

doi:10.16420/j.issn.0513-353x.2023-0690

摘要/Abstract

摘要：

为了探究刺槐（Robinia pseudoacacia）RpACBP3对光合生理的影响，利用农杆菌介导法将RpACBP3转入到烟草（Nicotiana tabacum）‘NC89’中，以野生型为对照，测定RpACBP3过表达烟草的相关指标。结果显示：获得了7个过表达株系。整体上，RpACBP3过表达株系表现出叶绿素a、叶绿素b、总叶绿素显著增加，类胡萝卜素降低；初始荧光（F_o）和非光化学淬灭系数（q_N）下降，最大光化学效率（F_v/F_m）和实际光化学量子效率（Φ_PSII）上升；光合速率（P_n）和胞间CO₂浓度（C_i）上升，气孔导度（G_s）和蒸腾速率（T_r）下降；气孔长度、气孔面积等明显上升。相关性分析显示，大多数指标之间存在显著相关关系；随机森林回归显示，RpACBP3过表达对烟草叶绿素a、Φ_PSII、C_i和气孔面积等指标的影响较大。综上所述，RpACBP3通过提升叶绿素含量、提高光合系统对光能的吸收和转化效率、促进气孔发育等途径来提高烟草植株的光能利用率。

关键词: 刺槐, 烟草, RpACBP3, 光合特性, 气孔

Abstract:

To investigate the regulatory effects of RpACBP3 from Robinia pseudoacacia on the photosynthetic-physiological functions of Nicotiana tabacum，this gene was transferred into N. tabacum‘NC89’via Agrobacterium-mediated method，and the relevant indices of RpACBP3 over-expressing tobacco plants were measured using wild-type plants as the control. The results showed that seven strains of N. tabacum with RpACBP3 over-expression were obtained in this experiment. Compared to those in wild-type plants，chlorophyll a，chlorophyll b，and total chlorophyll content considerably increased and carotenoid content decreased in RpACBP3 over-expression strains. Additionally，some indices such as initial fluorescence（F_o），non-photochemical quenching coefficient（q_N）decreased，maximum photochemical efficiency（F_v/F_m），actual photochemical quantum efficiency（Φ_PSII），photosynthetic rate （P_n），and intercellular CO₂ concentration（C_i）increased，whereas others such as stomatal conductance（G_s），transpiration rate（T_r）decreased，stomatal length，and stomatal area increased in RpACBP3-over- expressing-strains. Correlation analysis revealed significant correlations between most of the indicators，and random forest regression showed that RpACBP3 overexpression had the greatest effects on tobacco indices such as chlorophyll a content，Φ_PSII，C_i，and stomatal area，respectively. In summary，the RpACBP3 over-expression enhanced the light energy utilization of tobacco plants by increasing the chlorophyll content，improving the efficiency of light absorption and transformation by the photosystem，and promoting the stomatal development.

Key words: Robinia pseudoacacia, Nicotiana tabacum, RpACBP3, photosynthetic characteristics, stomata

张松彦, 迭鹏翔, 宋梦婷, 李志坚, 周建. 烟草过表达刺槐RpACBP3对光合生理的影响[J]. 园艺学报, 2024, 51(9): 2155-2167.

ZHANG Songyan, DIE Pengxiang, SONG Mengting, LI Zhijian, ZHOU Jian. Effect of Overexpression of Robinia pseudoacacia RpACBP3 Gene on Photosynthetic Physiological Characteristics of Nicotiana tabacum[J]. Acta Horticulturae Sinica, 2024, 51(9): 2155-2167.

图/表 10

表1 试验中所用的引物

Table 1 The primers used in this experiment

用途 Function	引物名称 Primer name	序列（5′-3′） Sequence
转基因阳性苗鉴定Identification of transgenic positive seedlings	OE-RpACBP3	F：GAGAACACGGGGGACTCTAGAATGGAGCTTGTAACTGCAAGTG R：CGATCGGGGAAATTCGAGCTCTCATTTCTTTACATTGTTCTC
qRT-PCR	NtActin	F：ACCTCTATGGCAACATTGTGCTCAG R：CTGGGAGCCAAAGCGGTGATT
qRT-PCR	qRpACBP3	F：ACCGGTACACGGTTCAAACA R：GACAATGTCTGGTTTCGCCG

图1 烟草转刺槐RpACBP3株系的PCR检测 M：DNA marker DL2000；P：阳性质粒对照；N：阴性对照；WT：野生型植株；OE：阳性株系。下同。

Fig. 1 PCR assay of Nicotiana tabacum-transformed Robinia pseudoacacia RpACBP3 strains M：DNA marker DL2000；P：Positive plasmid control；N：Negative control；WT：Wild type plants；OE：Positive lines. The same below.

图2 烟草转刺槐RpACBP3株系（OE）及野生型（WT）的qRT-PCR检测不同小写字母代表各株系间差异达到显著水平（P < 0.05）。下同。

Fig. 2 qRT-PCR detection of Nicotiana tabacum trans-Robinia pseudoacacia RpACBP3 strains（OE）and wild type（WT） Different lowercase letters represent the significant difference between the lines（P < 0.05）. The same below.

图3 烟草转刺槐RpACBP3株系（OE）及野生型（WT）的叶绿素与类胡萝卜素含量

Fig. 3 Chlorophyll and carotenoid content of Nicotiana tabacum turned Robinia pseudoacacia RpACBP3 strain（OE）and wild type（WT）

图4 烟草转刺槐RpACBP3株系（OE）及野生型（WT）的叶绿素荧光特性

Fig. 4 Chlorophyll fluorescence characterization of Nicotiana tabacum turned Robinia pseudoacacia RpACBP3 strain（OE）and wild type（WT）

图5 烟草转刺槐RpACBP3株系（OE）及野生型（WT）光合功能变化

Fig. 5 Changes in photosynthetic function of Nicotiana tabacum turned Robinia pseudoacacia RpACBP3 strain（OE）and wild type（WT）

表2 烟草转刺槐RpACBP3株系（OE）及野生型（WT）的气孔形态

Table 2 The in stomatal morphology of Nicotiana tabacum turned Robinia pseudoacacia RpACBP3 strain（OE）and wild type（WT）

株系 Lines	长度/μm Length	宽度/μm Width	面积/μm² Area
WT	17.87 ± 1.10 b	8.42 ± 0.65 bc	92.18 ± 13.78 c
OE1	19.27 ± 1.15 ab	8.29 ± 0.44 c	129.73 ± 9.12 abc
OE3	19.36 ± 0.18 ab	8.31 ± 0.22 c	118.34 ± 6.46 bc
OE5	21.35 ± 0.44 a	8.45 ± 0.20 bc	144.81 ± 10.84 ab
OE6	19.76 ± 0.38 ab	8.96 ± 0.55 abc	143.18 ± 6.47 ab
OE9	21.29 ± 0.56 a	9.79 ± 0.30 ab	159.23 ± 15.25 ab
OE10	21.80 ± 1.27 a	10.33 ± 0.63 a	175.04 ± 29.95 a
OE12	20.30 ± 0.74 ab	9.70 ± 0.19 abc	143.35 ± 6.35 ab

表3 烟草转刺槐RpACBP3株系各指标主成分分析

Table 3 Principal component analysis of indicators in Nicotiana tabacum transgenic Robinia pseudoacacia RpACBP3 strains %

指标 Index	第1主成分 Prin 1	第2主成分 Prin 2	第3主成分 Prin 3	第4主成分 Prin 4	第5主成分 Prin 5
方差贡献率Variance contribution rate	43.56	24.27	10.72	6.88	4.48
累计贡献率Cumulative contribution rate	43.56	67.83	78.55	85.43	89.91

图6 烟草转刺槐RpACBP3株系各指标的主成分分析（a）和随机森林回归分析（b）

Fig. 6 PCA（a）and random forest regression analysis（b）of indicators in Nicotiana tabacum transgenic Robinia pseudoacacia RpACBP3 strains

图7 烟草转刺槐RpACBP3株系各指标的相关性（a）和聚类热图（b）图a中红色代表正相关，蓝色代表负相关，颜色越深，相关性越大。

Fig. 7 Correlation（a）and cluster heat map（b）of indicators in Nicotiana tabacum transgenic Robinia pseudoacacia RpACBP3 strains In figure a，red represents positively correlated，and blue represents negatively correlated. The darker the color，the larger the correlation.

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