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园艺学报 ›› 2022, Vol. 49 ›› Issue (9): 1895-1906.doi: 10.16420/j.issn.0513-353x.2021-0700

• 研究论文 • 上一篇    下一篇

茄子生长素响应因子SmARF5对分枝发育的影响

胡若琳1, 王佳丽1, 杨慧勤1, 袁超1, 牛义1,*(), 汤青林1, 魏大勇1, 田时炳2, 杨洋2, 王志敏1,*()   

  1. 1西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室,重庆 400715
    2重庆市农业科学院蔬菜花卉研究所,重庆 400055
  • 收稿日期:2021-10-16 修回日期:2022-05-17 出版日期:2022-09-25 发布日期:2022-10-08
  • 通讯作者: 牛义,王志敏 E-mail:niuy2001134@163.com;minzniwang_555@163.com
  • 基金资助:
    重庆市技术创新与应用发展专项重点项目(cstc2019jscx-gksbX0149);重庆市基础研究与前沿探索专项面上项目(cstc2019jcyj-msxmX0448);现代农业产业技术体系专项建设资金项目(CARS-23-A12);重庆市农业科学院绩效激励引导专项项目(cqaas2019jxj101)

Cloning and Functional Analysis of Auxin Response Factor Gene SmARF5 in Solanum melongena

HU Ruolin1, WANG Jiali1, YANG Huiqin1, YUAN Chao1, NIU Yi1,*(), TANG Qinglin1, WEI Dayong1, TIAN Shibing2, YANG Yang2, WANG Zhimin1,*()   

  1. 1Chongqing Key Laboratory of Olericulture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China
    2The Institute of Vegetables and Flowers,Chongqing Academy of Agricultural Sciences,Chongqing 400055,China
  • Received:2021-10-16 Revised:2022-05-17 Online:2022-09-25 Published:2022-10-08
  • Contact: NIU Yi,WANG Zhimin E-mail:niuy2001134@163.com;minzniwang_555@163.com

摘要:

以茄子(Solanum melongena)‘S12’为试验材料,同源克隆了生长素响应因子(auxin response factor ARF5)基因SmARF5,该基因开放阅读框全长2 793 bp,编码930个氨基酸,定位于细胞核。分别构建载体pGBKT7-SmARF5-Full和截短体pGBKT7-SmARF5-674aa进行酵母转化试验。结果表明,SmARF5具有转录激活活性,SmARF5全长可以与SmIAA16和SmIAA26蛋白互作,截短体SmARF5-674aa不与SmIAA16和SmIAA26互作。构建过表达载体pCAMBIA-2301G-SmARF5并转化入烟草。结果表明,与野生型相比,SmARF5转基因烟草植株出现明显分枝,茎粗增大。以上结果表明SmARF5可能通过生长素信号转导途径在植物分枝形成过程中发挥重要作用。

关键词: 茄子, SmARF5, 生长素, 分枝

Abstract:

SmARF5 gene was cloned from the eggplant cultivar‘S12’. The open reading frame is 2 793 bp,and it encodes a 930 aa protein that located in the nucleus. The pGBKT7-SmARF5-Full and pGBKT7-SmARF5-674aa vectors were constructed. The transcriptional activation activity of SmARF5 protein was analyzed and showed by yeast transformation. The full length of SmARF5 could interact with SmIAA16 and SmIAA26 proteins. The truncated SmARF5-674aa did not interact with SmIAA16 or SmIAA26. The pCAMBIA-2301G-SmARF5 overexpression vector was constructed and genetically transformed into tobacco plants. Compared with the wild type,obvious branches and the stem thickness were increased in the transgenic plants. The above results indicated that the SmARF5 transcription factor might play an important role in the formation of plant branches through the auxin signal transduction pathway.

Key words: Solanum melongena, SmARF5, auxin, branching

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