园艺学报 ›› 2016, Vol. 43 ›› Issue (7): 1257-1266.doi: 10.16420/j.issn.0513-353x.2015-0923

• 果树 • 上一篇    下一篇


邵佳蓉,宋春波,卞 坤,陈 伟,杨震峰*   

  1. (浙江万里学院生物与环境学院,浙江宁波 315100)
  • 出版日期:2016-07-25 发布日期:2016-07-25

Expression Responses of SUMO E3 Ligase(SIZ1)to Low Temperature Stress and Exogenous Melatonin in Postharvest Peach Fruit

SHAO Jia-rong,SONG Chun-bo,BIAN Kun,CHEN Wei,and YANG Zhen-feng*   

  1. (College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo,Zhejiang 315100,China)
  • Online:2016-07-25 Published:2016-07-25


为了探索SUMO E3连接酶(SIZ1)基因与桃(Prunus persica L. Batsch)果实采后低温贮藏期间冷害发生的关系,对桃基因组中的SIZ1基因进行生物信息学分析,同时采用qRT-PCR分析该基因在‘湖景蜜露’桃果实低温贮藏以及外源褪黑素减轻冷害进程中的表达特征。结果表明,PpSIZ1开放阅读框全长2 637 bp,编码878个氨基酸组成的蛋白质多肽。进化树分析发现,PpSIZ1与梅PmSIZ1的同源性最高,含有与拟南芥AtSIZ1相似的SAP、PHD、PINIT、SP-RING和SXS保守结构域,属于SUMO E3连接酶家族。qRT-PCR分析表明,低温(0 ℃)胁迫能诱导桃果实PpSIZ1表达水平的提高;100和200 μmol · L-1褪黑素处理能显著减轻桃果实低温贮藏期间冷害的发生,其中100 μmol · L-1褪黑素处理抑制冷害的效果最好,这种效果可能与PpSIZ1介导的SUMO化有关。

关键词: 桃, 果实, 冷害, 褪黑素, PpSIZ1, SUMO E3连接酶


To explore the role of SUMO E3 ligase(SIZ1)gene in chilling injury incidence of postharvest peach fruit,PpSIZ1 gene was identified and analyzed by bioinformatics. Furthermore,the effects of low temperature storage and exogenous melatonin treatments on PpSIZ1 expression were investigated in‘Hujing Milu’peach fruit during storage. Results showed that SIZ1 gene was identified in the peach genome and named as PpSIZ1,and the ORF of PpSIZ1 was 2 637 bp which encoded a predicted protein of 878 amino acids. Phylogenetic analysis indicated that PpSIZ1shared high similarity homology with PmSIZ1. Bioinformatics analysis showed that PpSIZ1 possessed predicted SAP,PHD,PINIT,SP-RING,and SXS domains,which were highly conserved in AtSIZ1,indicated PpSIZ1 could be classified with SUMO E3 Ligase. Chilling injury index of the fruit stored at 0 ℃ increased with the prolongation of storage. Exogenous melatonin treatments(100 and 200 μmol · L-1)could significantly inhibited chilling injury incidence of peach fruit stored at 0 ℃,and 100 μmol · L-1 melatonin treatment exhibits the best inhibitory effect. Low temperature stress at 0 ℃ could induce the expression of PpSIZ1,and the transcription level of PpSIZ1 was strongly up-regulated when treated with 100 μmol · L-1 melatonin. These results suggest that PpSIZ1 protein possibly functioned as SUMO E3 ligase that facilitated the SUMO modification pathway,which could be associated with chilling tolerance in cold-stored peach fruit.

Key words: peach, fruit, chilling injury, melatonin, PpSIZ1, SUMO E3 ligase