关键词: 甘蓝, 自交不亲和性, 反义MLPK基因

Abstract: An antisense MLPK gene controlled by a stigma-specific promoter SLR of Brassica oleracea was introduced into a self-incompatibility B. oleracea‘TF’through Agrobacterium mediated transformation. Real-time PCR expression analysis of the MLPK gene in T0 generation transgenic cabbage plants was performed，significant different transcription levels of the MLPK gene in transgenic plants were observed，and the transcription level of MLPK in open ?ower stigmas of the transgenic plants were greatly reduced compared with the wild type plants. In situ fluorescence microscopy results showed a large number of pollen germination on the stigmas of transgenic plants，and markedly increased pollen tubes penetrated the stigma after self-pollination of open ?ower，which leads to significantly increased numbers of seed. Moreover，both the flowering and bud stage compatibility index of transgenic plants were significantly higher than the wild type. The results demonstrated that down-regulation expression of the MLPK gene in stigma can result in a partial breakdown of B. oleracea self-incompatibility，and promoteseed setting after self-pollination during flowering stage.

Key words: Brassica oleracea var. capitata, self-incompatibility, antisense MLPK gene