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园艺学报 ›› 2009, Vol. 36 ›› Issue (4): 583-586.

• 研究简报 • 上一篇    下一篇

结球甘蓝—大白菜异源三倍体小孢子培养的研究

张巍巍;申书兴*;王彦华; 陈雪平;轩淑欣;罗双霞;李晓峰
  

  1. (河北农业大学园艺学院, 河北保定071001)
  • 收稿日期:2008-10-17 修回日期:2009-03-24 出版日期:2009-04-25 发布日期:2009-04-25
  • 通讯作者: 申书兴

Studies on Isolated Microspore Culture of the Allotriploid Hybrids BetweenCabbage and Chinese Cabbage

ZHANG Wei-wei, SHEN Shu-xing*, WANG Yan-hua,CHEN Xue-ping, XUAN Shu-xin, LUO Shuang-xia,and LI Xiao-feng   

  1. (College of Horticulture, Agricultural University of Hebei, B aoding, Hebei 071001, China)
  • Received:2008-10-17 Revised:2009-03-24 Online:2009-04-25 Published:2009-04-25
  • Contact: SHEN Shu-xing

摘要: 为创建结球甘蓝—大白菜异附加系, 以大白菜二倍体(AA, 2n=2x=20) 与结球甘蓝四倍体
(CCCC, 2n=4x=36) 杂交获得的8个异源三倍体(ACC, 2n=3x=28) 单株系为试材, 进行游离小孢子培养研究, 获得再生植株, 并对其进行了染色体数目鉴定和性状调查。结果表明, 异源三倍体(ACC) 的小孢子诱导成胚的能力低于双亲; 在NLN中添加15%的蔗糖和0.1 mg·L-1 6-BA、0.2 mg·L-1 2, 4-D、0.05 mg·L-1 KT的培养基上小孢子胚胎发生最高为0.0250个·蕾-1, 8个株系间有差异, 平均为0.0100个·蕾-1; 胚胎再生率只有33.3%。小孢子再生植株染色体数在14~27条之间, 田间性状表现多样。

关键词: 结球甘蓝, 大白菜, 小孢子培养, 胚状体, 异附加系

Abstract: Microspore culture of 8 allotriploid hybrids between diploid Chinese cabbage (AA, 2n=2x=20) and autotetrap loid cabbage (CCCC, 2n=4x=36) was performed in order to create the alien addition lines. The results showed that embryo p roduction frequency of the allotrip loid hybrids (ACC) was lower than
their parents. In liquid medium NLN with 15% sucrose and 0.1 mg·L-1 6-BA, 0.2 mg·L-1 2, 4-D, 0.05mg·L-1 KT, the 8 lines were different, the highest of embryo production was 0.0250 embryos per bud, the average was 0.0100 embryos per bud. The p lant regeneration frequency of embryoids was only 33.3%. The chromosome number of plantlets derived from microspore culture varied from 14 to 27. The morphological characters were diverse among the different plants.

Key words: cabbage, Chinese cabbage, microspore culture, embryo, alien addition lines

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