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园艺学报 ›› 2008, Vol. 35 ›› Issue (9): 1285-1290.

• 蔬菜 • 上一篇    下一篇

大白菜初级三体的配子形成及传递率研究

申书兴1,2 ;侯喜林1*;罗双霞2
  

  1. (1南京农业大学作物遗传与种质资源创新国家重点实验室,园艺学院,南京210095;2河北农业大学园艺学院,河北保定071001)
  • 收稿日期:2008-03-19 修回日期:2008-07-14 出版日期:2008-09-25 发布日期:2008-09-25
  • 通讯作者: 侯喜林

Studies on the Gamete Formation and Transmission Rate of a Set of Primary Trisomics in Chinese Cabbage

SHEN Shu-xing 1,2 ,HOU Xi-lin1*,and LUO Shuang-xia2   

  1. ( 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultral University, Nangjing 210095, China; 2College of Horticulture, Hebei Agricultural University,Baoding ,Hebei 071001, China)
  • Received:2008-03-19 Revised:2008-07-14 Online:2008-09-25 Published:2008-09-25
  • Contact: HOU Xi-lin

摘要:

大白菜三体的n+1配子传递率的测定是利用三体进行遗传分析的重要参数。本研究以大白菜初级三体系为亲本,分别与二倍体正反交,测定n+1雌雄配子传递率,并将各三体雄配子形成过程中染色体的减数分裂行为及花粉特性进行了观察分析。研究结果表明:大白菜初级三体系除10号三体(Tri-10)外,其他三体均能通过n+1雌雄配子传递,但传递率在不同三体间以及雌雄配子间有差异。各三体在雄配子形成过程中,染色体的减数分裂行为从三价体的形成到后期Ⅰ、后期Ⅱ的分离等方面都存在异常,这些共同影响n+1雄配子的传递率。

关键词: 大白菜, 初级三体, 染色体, 传递率

Abstract: Transmission rate of n+1 gametes is an important parameter for genetic analysis of trisomics. The transmission rate of n+1 gametes identified by analyzing the progenies of a set of primary trisomics in Chinese cabbage reciprocal crosses with diploid respectively .The chromosome behaviors of each trisomics during meiotic divisions were studied. The results showed that n+1 female and male gametes of the trisomics could transmitted except of Tri-10, whereas transmission rate among various trisomics were different. The main influencing factor on n+1 male gamete transmission rate were the trivalent formation and abnormal chromosome segregation at anaphaseⅠandⅡ during meiotic divisions of each trisomics.

Key words: Chinese cabbage, primary trisomics, chromosome, transmission rate

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