香蕉果实贮藏冷害与NAD激酶活性变化的关系

园艺学报 ›› 2008, Vol. 35 ›› Issue (10): 1425-1430.

香蕉果实贮藏冷害与NAD激酶活性变化的关系

刘彩霞¹,顾采琴^1*,朱冬雪²

(¹广州大学化学化工学院食品系, 广州 510006；²广州大学生命科学学院，广州 510006）

收稿日期:2008-05-27 修回日期:2008-08-26 出版日期:2008-10-25 发布日期:2008-10-25
通讯作者: 顾采琴

The Relationship Between Chilling Injury and NADK Activity in Postharvest Banana Fruits

LIU Cai-xia¹;GU Cai-qin^1*;and ZHU Dong-xue²

(¹School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China; ²College of Life Science, Guangzhou University, Guangzhou 510006, China)

Received:2008-05-27 Revised:2008-08-26 Online:2008-10-25 Published:2008-10-25
Contact: GU Cai-qin

摘要/Abstract

摘要： 以‘巴西'香蕉果实为试材，研究了果实采后在22℃和6℃下贮藏期间NAD激酶(NADK)活性与冷害发生情况，膜渗透性，丙二醛(MDA)含量，多酚氧化酶(PPO)以及苯丙氨酸解氨酶(PAL)活性变化的关系。结果表明，6℃下贮藏3d 时果实出现冷害症状, 11d时冷害加重，同时NAD激酶活性、膜渗透性、MDA含量和PPO活性均始终高于22℃贮藏的, 并在11d时达到高峰，而PAL活性则呈下降趋势，其活性始终低于22℃贮藏的。6℃下用NAD激酶激活剂氯化钙处理香蕉果实后，NADK受到激活，冷害程度加重，而用钙离子通道阻塞剂异博定（verapamil，Vp）处理后则抑制了NADK 活性，果实冷害症状延迟4 d 出现，冷害程度减弱，说明香蕉果实冷害发生与NADK 活性变化密切相关。

关键词: 香蕉, 果实, 冷害, NAD激酶, 贮藏

Abstract:

Changes of activity of NAD kinase, polyphenol oxidase (PPO) and phenylalanine ammonialyase (PAL) , chilling injury and relative conductivity, MDA content of postharvest banana fruits storage at 22℃ and 6℃ were studied. The results showed that the fruits at 6 ℃ for three days occured visible chilling injury and became more serious for 11 days. The activity of NAD kinase at 6℃ was higher than that at 22℃ during storage. Higher relative conductivity, MDA content and PPO activity were observed at 6℃ than those at 22℃ and reached peak on the 11th day. However, the PAL activity at 6℃ was lower than that at 22℃ during storage. Pretreatment with CaCl2 could enhance the activity of NAD kinase and increase chilling injury of banana fruits at 6℃, but pretreatment with Vp had reverse effect. The results suggested that the chilling injury of postharvest banana fruits was highly related to the change of NADK activity.

Key words: banana, fruit, chilling injury, NAD kinase, Storage

中图分类号:

S 668.1

刘彩霞;顾采琴;朱冬雪. 香蕉果实贮藏冷害与NAD激酶活性变化的关系 [J]. 园艺学报, 2008, 35(10): 1425-1430.

LIU Cai-xia;GU Cai-qin;and ZHU Dong-xue. The Relationship Between Chilling Injury and NADK Activity in Postharvest Banana Fruits [J]. ACTA HORTICULTURAE SINICA, 2008, 35(10): 1425-1430.

[1]	张欣, 漆艳香, 曾凡云, 王艳玮, 谢培兰, 谢艺贤, 彭军. 香蕉枯萎病菌Dicer-like基因的功能分析[J]. 园艺学报, 2023, 50(2): 279-294.
[2]	梁嘉莉, 吴启松, 陈广全, 张荣, 徐春香, 冯淑杰, . 香蕉叶斑病病原菌芭蕉新拟盘多毛孢的鉴定[J]. 园艺学报, 2023, 50(2): 410-420.
[3]	杨植, 张川疆, 杨芯芳, 董梦怡, 王振磊, 闫芬芬, 吴翠云, 王玖瑞, 刘孟军, 林敏娟. 枣与酸枣杂交后代果实遗传倾向及混合遗传分析[J]. 园艺学报, 2023, 50(1): 36-52.
[4]	邵凤清, 罗秀荣, 王奇, 张宪智, 王文彩. 果实成熟过程中的DNA甲基化调控研究进展[J]. 园艺学报, 2023, 50(1): 197-208.
[5]	王燕, 刘针杉, 张静, 杨鹏飞, 马蓝, 王旨意, 涂红霞, 杨绍凤, 王浩, 陈涛, 王小蓉. 中国樱桃杂交F₁代花和果实若干性状遗传倾向分析[J]. 园艺学报, 2022, 49(9): 1853-1865.
[6]	杨兴玉, 许林兵, 吴元立, 邱迪洋, 范琳琳, 黄秉智. 香蕉杂交种‘粉杂1号’ABBB基因组鉴定[J]. 园艺学报, 2022, 49(9): 1991-1997.
[7]	蒙小玉, 穆悦, 胡杨, 吴潇, 朱辰, 王慧敏, 陶书田, 张绍铃, 殷豪. 几种果实表面积的三维激光扫描测定[J]. 园艺学报, 2022, 49(9): 1998-2006.
[8]	张悦, 索玉静, 孙鹏, 韩卫娟, 刁松锋, 李华威, 张嘉嘉, 傅建敏, 李芳东. 柿种质资源果实形态多样性分析[J]. 园艺学报, 2022, 49(7): 1473-1490.
[9]	魏晓羽, 王跃进. 中国野生葡萄果皮解剖结构与白粉病抗性的相关性研究[J]. 园艺学报, 2022, 49(6): 1200-1212.
[10]	路涛, 余宏军, 李强, 蒋卫杰. 叶果量调控对番茄生长发育、果实品质和产量的影响[J]. 园艺学报, 2022, 49(6): 1261-1274.
[11]	向妙莲, 吴帆, 李树成, 马巧利, 王印宝, 肖刘华, 陈金印, 陈明. 外源褪黑素调控活性氧代谢诱导梨果实抗采后黑斑病[J]. 园艺学报, 2022, 49(5): 1102-1110.
[12]	叶泗洪, 刘飞, 添长久. 黄秋葵新品种‘皖秋葵4号’[J]. 园艺学报, 2022, 49(5): 1175-1176.
[13]	李黎, 冯丹丹, 潘慧, 李文艺, 邓蕾, 汪祖鹏, 钟彩虹. 猕猴桃花粉灭菌方法比较及对果实品质的影响[J]. 园艺学报, 2022, 49(4): 769-777.
[14]	王宏, 杨王莉, 蔺经, 杨青松, 李晓刚, 盛宝龙, 常有宏. 早熟砂梨‘苏翠1号’与其亲本‘翠冠’‘华酥’成熟果实差异代谢产物及差异基因比较分析[J]. 园艺学报, 2022, 49(3): 493-508.
[15]	刘文欢, 邱芳颖, 王娅, 陈朗, 马岩岩, 吕强, 易时来, 谢让金, 郑永强. 枯草芽孢杆菌液态肥对柑橘养分吸收和果实品质的影响[J]. 园艺学报, 2022, 49(3): 509-518.