白菜Pol胞质雄性不育系和保持系蕾期基因表达差异的SRAP分析

园艺学报 ›› 2007, Vol. 34 ›› Issue (3): 655-658.

白菜Pol胞质雄性不育系和保持系蕾期基因表达差异的SRAP分析

邓晓辉;张蜀宁;侯喜林^*

(南京农业大学作物遗传与种质创新国家重点实验室, 南京210095)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-25 发布日期:2007-06-25

Differential Expression Analysis of Bud of Pol CMS and Its Maintainter Lineof Brassica rapa ssp. chinensis Through SRAP

DENG Xiao-hui, ZHANG Shu-ning, and HOU Xi-lin^*

(State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095,China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

摘要/Abstract

摘要： 将SRAP技术用于基因差异表达研究, 用30对SRAP引物组合对白菜Pol胞质雄性不育系和
保持系的cDNA进行扩增, 得到两条特异片段BcCMS1、BcCMS2。对特异片段克隆测序分析后发现, BcC
MS1与大白菜细胞生成相关染色体的19个氨基酸有63%的相似性, BcCMS2与芥蓝抑制开花的基因FLC3
部分碱基有88%的相似性。这两个序列在GenBank中的登录号分别为DQ914921、DQ914922。此结果显示
在胞质雄性不育中细胞质基因改变了部分核基因的表达。

关键词: 白菜, 胞质雄性不育, SRAP, 基因表达

Abstract: cDNA of Pol cytop lasmic male sterility (CMS) and its maintainer line were amp lified with 30
pairs of SRAP ( sequence related amp lified polymorphism) p rimers combination, and two fragmentsBcCMS1 and
BcCMS2 were obtained. Information from cloning and sequencing of these two bands indicated thatBcCMS1 was
of 63% of homology with 19 amino acids of chromosome related to cytogenetic of B rassica rapa, BcCMS2 was of
88% of homologywith the FLC3 gene for inhibiting flower gene of Brassica oleracea. The accession numbers for
these two sequences in GenBank were DQ914921 and DQ914922, respectively. The results obtained suggested
that the exp ression of the nuclear gene in cytogenetic male sterility was affected by the cytop lasmic gene. The
SRAP technique was firstly successfully adop ted in the research of gene differential exp ression.

Key words: Brassica rapa ssp. chinensis, CMS, SRAP, Geneexp ression

邓晓辉;张蜀宁;侯喜林. 白菜Pol胞质雄性不育系和保持系蕾期基因表达差异的SRAP分析[J]. 园艺学报, 2007, 34(3): 655-658.

DENG Xiao-hui;ZHANG Shu-ning;and HOU Xi-lin. Differential Expression Analysis of Bud of Pol CMS and Its Maintainter Lineof Brassica rapa ssp. chinensis Through SRAP[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 655-658.

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