园艺学报 ›› 2005, Vol. 32 ›› Issue (02): 314-317.

• 研究报告 • 上一篇    下一篇

高温胁迫对黄瓜花药中Ca2+ 分布、ABA含量及蛋白质合成的影响


  1. (1 扬州大学农学院园艺系, 扬州225009; 2 南京农业大学园艺学院, 南京210095)
  • 收稿日期:2004-05-08 修回日期:2004-07-05 出版日期:2005-04-25 发布日期:2005-04-25

Effect of Heat Stress on Calcium Distribution, ABA Contents and the Synthesis of Proteins in Cucumber Anthers

Zhang Yuhua1;Zhu Yuelin2;Miao Minmin1

  1. (1Department of Horticulture, College of Agriculture, Yangzhou University, Yangzhou 225009; 2College of Horticulture, Nanjing Agriculture University, Nanjing 210095)
  • Received:2004-05-08 Revised:2004-07-05 Online:2005-04-25 Published:2005-04-25

摘要: 经45℃处理1 h后黄瓜热敏品系新泰密刺单核花粉期花药胞内Ca2+水平上升较耐热品系JY24
快, 但在温度恢复28℃后其钙稳态恢复较慢; 45℃处理1 h后新泰密刺花药内源ABA含量上升幅度比JY24大, 恢复28℃后下降速度也较快; 高温处理未能造成此期黄瓜花药新蛋白质的合成, 但蛋白(MW 70 kD,p I 5.7和MW 45.5 kD, p I 5.6) 的表达热激后大幅度上调, 且在JY24中的表达水平显著高于新泰密刺。上述结果表明, Ca2+和ABA均参与了黄瓜花药对高温胁迫的反应调节; 黄瓜单核花粉期花药中热激蛋白的合成至少已被部分抑制。

关键词: 黄瓜, 高温, 花药, 钙, ABA, 蛋白质双向电泳

Abstract: Under 45℃ treatment, Ca2+ precipitates in pollen and anther cells increase more quickly in cucumber (Cucumis sativus L. ) heat-sensitive line Xintaimici than in heat-tolerant line JY24. While the temperature recover to 28℃, the Ca2+ precipitates quantity drop to the normal levelmore tardily in Xitaimici
anther cells. EndogenousABA contents in two line anthers also ascend significantly after 1 h 45℃ treatment,especially in Xintaimici. When the temperature return to 28℃, ABA in Xitaimici anthers step down to the
control level, while JY24 anthers can maintain a higher ABA content within 2 h. No any additional protein points can be seen in the IEF-SDS-PAGE maps of cucumber anthers under 45℃ stress comparing with 28℃, although the expression of some proteins, such as (MW 70 kD, P I 5.7) and (MW 45.5 kD, PI 5.6) wereup regulated remarkably after heat treatment ( even higher in JY24 than in Xintaimici). The results indicated both Ca2+ and ABA signalswere involved in the response of cucumber anthers to heat-stress. The synthesis of
HSPs in cucumber uninucleate-binucleate-pollen-phase antherswere at least in part inhibited under 45℃.

Key words: Cucumber, High temperature, Anther, Ca2 +, ABA, 2D PAGE