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园艺学报 ›› 2001, Vol. 28 ›› Issue (3): 189-193.

• 研究论文 •    下一篇

草莓主栽品种再生和转化的研究

张志宏1;吴禄平1;代红艳1;王国英2;赵天永2;毕晓颖1;杜国栋1   

  1. (1 沈阳农业大学园艺系, 沈阳110161 ; 2 中国农业大学生物技术国家重点实验室, 北京100094)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-06-25 发布日期:2001-06-25

Regeneration and Transformation in Vitro of the Strawberry Varieties

Zhang Zhihong1, Wu Luping1, Dai Hongyan1, Wang Guoying2, Zhao Tianyong1, Bi Xiaoying1, and Du Guodong1   

  1. (1 Department of Horticulture , Shenyang Agricultural University , Shenyang 110161; 2 College of Biology , China Agricultural University , Beijing 100094)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-06-25 Published:2001-06-25

摘要: 建立草莓主栽品种高效、稳定的离体再生体系和遗传转化体系, 获得了转基因植株。‘弗吉尼亚’和‘森嘎拉’的叶片离体再生芽频率达到100 %。试管苗叶片与农杆菌菌株EHA105 共培养3 d。共培养后的叶片在附加卡那霉素40 mg/L 的再生培养基上选择培养4周后, 外植体再生出转化芽, 弗吉尼亚的转化芽再生频率可达6. 8 %。采用组织化学染色法对随机选取的10 个GUS 基因转化植株进行基因表达测定, 结果5 个植株强烈表达GUS 活性。转bar 基因植株在附加除草剂草丁膦10 mg/L 的培养基上能够正常分化, 在田间对草丁膦表现出强烈抗性。转基因植株开花、结果正常。

关键词: 草莓, 再生, 转化, 抗除草剂

Abstract: An efficiency and stable regeneration system in vitro and genetic transformation system for the commercial strawberry varieties were developed , and transgenic plants were obtained. Bud regeneration percentage of leaf pieces of strawberry varieties ‘Tudla’and ‘Senga Sengana’reached 100 %. Leaf pieces from in vitrogrown plants‘Tudla’were cocultivated for 3 days with Agrobacterium tumef aciens EHA105. After the cocultivated leaf pieces were incubated on regeneration medium
containing 40 mg/ L kanamycin for 4 weeks , the transformed buds appeared on the explants. The regeneration percentage of transformation buds of strawberry variety‘Tudla’reached 6. 8 %. The result of histochemical staining of GUS gene showed , of the 10 independent plants transformed with GUS gene , 5 expressed GUS activity strongly. The plants transformed with bar gene were able to differentiation on the medium containing glufosinate 10 mg/ L , and expressed strongly resistance to glufosinate
on field. The transgenic plants flowered and fruited normally.

Key words: Strawberry, Regeneration, Transformation, Resistance to herbicide

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