光和温度对开花时间基因可变剪接的影响研究进展

doi:10.16420/j.issn.0513-353x.2023-0946

园艺学报 ›› 2024, Vol. 51 ›› Issue (8): 1949-1963.doi: 10.16420/j.issn.0513-353x.2023-0946

光和温度对开花时间基因可变剪接的影响研究进展

文宋琴¹, 李佳霖¹, 池卓恒¹, 夏燕¹, 王淑明¹, 吴頔¹, 郝雅雯², 郭启高¹, 梁国鲁¹, 景丹龙¹^,^*()

¹ 西南大学园艺园林学院，长江上游农业生物安全与绿色生产教育部重点实验室，西南大学农业科学研究院，南方山地作物逆境生物学国家级培育基地，重庆 400715
² 重庆南开中学，重庆 400030

收稿日期:2024-01-19 修回日期:2024-05-31 出版日期:2024-08-25 发布日期:2024-08-22
通讯作者:
^*E-mail：jingdanlong@swu.edu.cn
基金资助:
国家自然科学青年基金项目(32102321); 重庆市科技局项目(CSTB2022NSCQ-MSX1541); 重庆市科技局项目(cstc2021jscx-gksbX0010); 中央高校基本科研业务费—能力提升项目(SWU-KT22055); 国家级大学生创新创业训练计划项目(202110635078); 重庆市中小学创新人才培养工程项目(CY230202)

The Role of Light and Temperature Related Genes Alternative Splicing in Regulating Plant Flowering Time

WEN Songqin¹, LI Jialin¹, CHI Zhuoheng¹, XIA Yan¹, WANG Shuming¹, WU Di¹, HAO Yawen², GUO Qigao¹, LIANG Guolu¹, JING Danlong¹^,^*()

¹ Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River（Ministry of Education），College of Horticulture and Landscape Architecture，Southwest University; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land，Academy of Agricultural Sciences of Southwest University，Chongqing 400715，China
² Chongqing Nankai Middle School，Chongqing 400030，China

Received:2024-01-19 Revised:2024-05-31 Published:2024-08-25 Online:2024-08-22

摘要/Abstract

摘要：

可变剪接（AS）是植物生长发育过程中最有效的基因转录调控方式之一。在植物中，大量基因经历了可变剪接；调控开花时间基因的可变剪接受到光和温度信号的影响。本文中重点综述了可变剪接的类型、可变剪接的分子机制及在光与温度季节性变化下基因可变剪接调控开花时间的特性和功能，旨在提高对基因可变剪接参与调控植物开花时间机制的认识，为研究植物生长发育调控机制提供新思路。

关键词: 基因, 可变剪接, 光周期, 温度, 开花时间

Abstract:

Alternative splicing（AS）is a powerful method to regulate gene transcription during plant growth and development. In plants，many genes undergo alternative splicing，and alternative splicing of genes that control flowering time is influenced by light and temperature signals. In this article，the types of alternative splicing，the molecular mechanism of variable splicing，and the properties and functions of alternative splicing of genes in regulating flowering time under seasonal light and temperature changes were focused on studying，with the aim of understanding the mechanism to improve the splicing of gene variables involved in the regulation of flowering time in plants and to provide a new idea for studying the mechanism of regulation of plant growth and development.

Key words: gene, alternative splicing, photoperiod, temperature, flowering time

文宋琴, 李佳霖, 池卓恒, 夏燕, 王淑明, 吴頔, 郝雅雯, 郭启高, 梁国鲁, 景丹龙. 光和温度对开花时间基因可变剪接的影响研究进展[J]. 园艺学报, 2024, 51(8): 1949-1963.

WEN Songqin, LI Jialin, CHI Zhuoheng, XIA Yan, WANG Shuming, WU Di, HAO Yawen, GUO Qigao, LIANG Guolu, JING Danlong. The Role of Light and Temperature Related Genes Alternative Splicing in Regulating Plant Flowering Time[J]. Acta Horticulturae Sinica, 2024, 51(8): 1949-1963.

图/表 5

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物种 Species	基因 Gene	剪接类型 Types of alternative splicing	参考文献 Reference
拟南芥 Arabidopsis thaliana	PHYA	内含子保留，选择性供点 Intron retention，selective donor	Shikata et al.,2014
	CRY2	选择性受点Selective acceptor	Shikata et al.,2014；Zhang et al.,2015
	ZTL	内含子保留 Intron retention	Lamesch et al.,2012
	COP1	选择性受点 Selective acceptor	Zhou et al.,1998；Shikata et al.,2014
	HY5	内含子保留，选择性供点 Intron retention，selective donor	Zhang et al.,2015
	HYH	内含子保留，选择性受点 Intron retention，selective acceptor	Sibout et al.,2006
	LHY	内含子保留，外显子跳跃 Intron retention，exon skipping	Zhang et al.,2015
	CCA1	内含子保留，选择性供点 Intron retention，selective donor	Shang et al.,2017
	TOC1	内含子保留 Intron retention	Shang et al.,2017
	RVE8	选择性受点 Selective acceptor	Shikata et al.,2014；Mancini et al.,2016
	JMJD5	内含子保留 Intron retention	Shikata et al.,2014；Mancini et al.,2016
	TIC	内含子保留 Intron retention	Mancini et al.,2016
	CKB3	内含子保留，选择性受点Intron retention，selective acceptor	Mancini et al.,2016
	PIF6	内含子保留 Intron retention	Penfield et al.,2010
	SPA3	内含子保留 Intron retention	Shikata et al.,2014
	RRC1	外显子跳跃 Exon skipping	Xin et al.,2019
	BBX25	内含子保留 Intron retention	Xin et al.,2019
	PIF3	内含子保留 Intron retention	Dong et al.,2020
梨Pyrus spp.	ELF3	内含子保留，选择性供点Intron retention，selective donor	Xin et al.,2019；Wang et al.,2023

物种 Species	基因 Gene	剪接类型 Types of alternative splicing	参考文献 Reference
拟南芥 Arabidopsis thaliana	PHYA	内含子保留，选择性供点 Intron retention，selective donor	Shikata et al.,2014
	CRY2	选择性受点Selective acceptor	Shikata et al.,2014；Zhang et al.,2015
	ZTL	内含子保留 Intron retention	Lamesch et al.,2012
	COP1	选择性受点 Selective acceptor	Zhou et al.,1998；Shikata et al.,2014
	HY5	内含子保留，选择性供点 Intron retention，selective donor	Zhang et al.,2015
	HYH	内含子保留，选择性受点 Intron retention，selective acceptor	Sibout et al.,2006
	LHY	内含子保留，外显子跳跃 Intron retention，exon skipping	Zhang et al.,2015
	CCA1	内含子保留，选择性供点 Intron retention，selective donor	Shang et al.,2017
	TOC1	内含子保留 Intron retention	Shang et al.,2017
	RVE8	选择性受点 Selective acceptor	Shikata et al.,2014；Mancini et al.,2016
	JMJD5	内含子保留 Intron retention	Shikata et al.,2014；Mancini et al.,2016
	TIC	内含子保留 Intron retention	Mancini et al.,2016
	CKB3	内含子保留，选择性受点Intron retention，selective acceptor	Mancini et al.,2016
	PIF6	内含子保留 Intron retention	Penfield et al.,2010
	SPA3	内含子保留 Intron retention	Shikata et al.,2014
	RRC1	外显子跳跃 Exon skipping	Xin et al.,2019
	BBX25	内含子保留 Intron retention	Xin et al.,2019
	PIF3	内含子保留 Intron retention	Dong et al.,2020
梨Pyrus spp.	ELF3	内含子保留，选择性供点Intron retention，selective donor	Xin et al.,2019；Wang et al.,2023

剪接因子 Splicing factor	蛋白类型 Protein type	剪接形式 Forms of splicing	参考文献 Reference
U2AF65a/b	U2辅助因子的亚基 Subunits of U2 auxiliary factors	FLC内含子 FLC introns	Park et al.,2019a
BRR2a	U5小核糖体蛋白U5 snRNP	FLC内含子1、5、6 FLC intron1,5 and 6	Mahrez et al.,2016
RZ-1B，RZ-1C	hnRNP蛋白 hnRNP proteins	FLC内含子1 FLC intron 1	Wu et al.,2016
KHZ1，KHZ2	RNA结合蛋白 RNA-binding proteins	FLC内含子 FLC introns	Yan et al.,2020
PRP8	核心剪接体组件 Core spliceosome component	COOLAIR内含子 COOLAIR introns	Marquardt et al.,2014

剪接因子 Splicing factor	蛋白类型 Protein type	剪接形式 Forms of splicing	参考文献 Reference
U2AF65a/b	U2辅助因子的亚基 Subunits of U2 auxiliary factors	FLC内含子 FLC introns	Park et al.,2019a
BRR2a	U5小核糖体蛋白U5 snRNP	FLC内含子1、5、6 FLC intron1,5 and 6	Mahrez et al.,2016
RZ-1B，RZ-1C	hnRNP蛋白 hnRNP proteins	FLC内含子1 FLC intron 1	Wu et al.,2016
KHZ1，KHZ2	RNA结合蛋白 RNA-binding proteins	FLC内含子 FLC introns	Yan et al.,2020
PRP8	核心剪接体组件 Core spliceosome component	COOLAIR内含子 COOLAIR introns	Marquardt et al.,2014

光和温度对开花时间基因可变剪接的影响研究进展

The Role of Light and Temperature Related Genes Alternative Splicing in Regulating Plant Flowering Time

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光和温度对开花时间基因可变剪接的影响研究进展

The Role of Light and Temperature Related Genes Alternative Splicing in Regulating Plant Flowering Time

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