葡萄GRF/GIF家族基因对遗传转化再生效率的影响

doi:10.16420/j.issn.0513-353x.2024-0077

摘要/Abstract

摘要：

为研究葡萄GRF/GIF家族基因对遗传转化再生效率的影响，对在葡萄基因组中鉴定到10个VvGRF和4个VvGIF基因进行了研究。VvGRF含有家族保守结构域WRC和QLQ，VvGIF含有保守结构域SNH和QG。VvGRF和VvGIF过表达烟草培养1个月后再生情况明显优于空载对照。烟草中过表达VvGRF8-GIF2和VvGRF4-GIF2，再生效率高达73.58%和65.05%，是对照的2.4 ~ 2.7倍；过表达rVvGRF8-GIF2（miR396结合的靶位点突变后的VvGRF8-GIF2），再生效率提升效果最好，达到了83.43%，是对照的3.1倍；相对于VvGRF8-GIF2嵌合体的过表达转化，构建的VvGRF8、VvGIF2同源嵌合体VvGRF4-GIF2、VvGRF5-GIF2和VvGRF8-GIF3过表达后的促进再生效果相对较弱。综合试验结果，VvGRF8和VvGIF2有利于提升植物遗传转化的再生效率。

关键词: 葡萄, GRF, GIF, 遗传转化, 再生

Abstract:

In order to study the effect of GRF/GIF family genes on the efficiency of genetic transformation and regeneration，10 VvGRF and 4 VvGIF genes were identified in grape genome. VvGRF contains family conserved domains WRC and QLQ，VvGIF contains conserved domains SNH and QG. The regeneration of tobacco overexpressing VvGRF and VvGIF after one month of cultivation was significantly better than that the empty control. VvGRF8-GIF2 and VvGRF4-GIF2 are overexpressed in tobacco，and the regeneration efficiency is as high as 73.58% and 65.05%，which is 2.4-2.7 times that of control. Overexpression of rVvGRF8-GIF2（VvGRF8-GIF2 after mutation of miR396 binding target site）showed the best regeneration efficiency，reaching 83.43%，which was 3.1 times that of control. Compared with the overexpression transformation of VvGRF8-GIF2 chimera，the promotion effect of constructed VvGRF8，VvGIF2 homologous chimera VvGRF4-GIF2，VvGRF5-GIF2 and VvGRF8-GIF3 after overexpression is relatively weak. According to the experimental results，VvGRF8 and VvGIF2 can improve the regeneration efficiency of plant genetic transformation.

Key words: grape, GRF, GIF, genetic transformation, regeneration

李敏, 李思雨, 施紫涵, 陈爽, 徐炎, 刘国甜. 葡萄GRF/GIF家族基因对遗传转化再生效率的影响[J]. 园艺学报, 2025, 52(1): 51-65.

LI Min, LI Siyu, SHI Zihan, CHEN Shuang, XU Yan, LIU Guotian. Studies on the Efficiency of GRFs/GIFs for Genetic Transformation and Regeneration in Grapevine[J]. Acta Horticulturae Sinica, 2025, 52(1): 51-65.

图/表 10

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基因 Gene	基因ID Gene ID	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
VvGRF1	Vitvi02g00239	CCTGGTGGCGTCTATGAGTG	AGCCGCCATTCCTCCAGT
VvGRF2	Vitvi02g00449	CTTCCAAGATTGCCCGCCTT	GCAGCCAAGCATATCCCCAG
VvGRF3	Vitvi08g01498	AGCATTATCAGCCTGCTTTGTTG	TGACCAGTTACCACGTCCCT
VvGRF4	Vitvi09g00107	CCTATCACCTCAGGCCACCA	TCCACACCCTCCCTCATTCC
VvGRF5	Vitvi11g00092	AGGCCATTTTACCTTGCTGGC	AGCTCTCTGCCCCCATTCAC
VvGRF6	Vitvi15g00815	CCGCTTCGGTGTATTGCTTCT	CTGCTCAAGCTCACGCAACT
VvGRF7	Vitvi15g04517	CCATTCGGATTGTCGGGCTC	GCTCAGGGTCAGTGTTTCCG
VvGRF8	Vitvi16g04017	GGTCCGGAACCAACCAATCC	AGGCCATGTGCTGTCCAAAG
VvGRF9	Vitvi16g01354	TGTGGTAAGGTGGTGGGTGT	TCCCACGTTGTTGCAGTGTC
VvGRF10	Vitvi18g00623	CTGGTCAGCAAGAACAGCAG	AAGTTCCCAGAGCTGTAACCTGT
VvGIF1	Vitvi07g00563	TGTTCACTTTGCAGTTTGCAGC	ATCAGTGGTGACGTTGCTGG
VvGIF2	Vitvi14g02674	CATTTTGGACCACCACGGTAGA	TGGTATGCTAACTCCACACGC
VvGIF3	Vitvi15g00903	ATGCAGCAGAACCCCCAGAT	GCTGAGCTTGGTACTGTGC
VvGIF4	Vitvi16g01357	CCTTCATTTCCTCCCACCGC	GGTGGTTGGGCATCAGCAAT
GAPDH	F6GSG7_VITVI	CCAAGGCTGGAATAGCACTC	CCATGTGGACAATCAAGTCG

基因 Gene	基因ID Gene ID	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
VvGRF1	Vitvi02g00239	CCTGGTGGCGTCTATGAGTG	AGCCGCCATTCCTCCAGT
VvGRF2	Vitvi02g00449	CTTCCAAGATTGCCCGCCTT	GCAGCCAAGCATATCCCCAG
VvGRF3	Vitvi08g01498	AGCATTATCAGCCTGCTTTGTTG	TGACCAGTTACCACGTCCCT
VvGRF4	Vitvi09g00107	CCTATCACCTCAGGCCACCA	TCCACACCCTCCCTCATTCC
VvGRF5	Vitvi11g00092	AGGCCATTTTACCTTGCTGGC	AGCTCTCTGCCCCCATTCAC
VvGRF6	Vitvi15g00815	CCGCTTCGGTGTATTGCTTCT	CTGCTCAAGCTCACGCAACT
VvGRF7	Vitvi15g04517	CCATTCGGATTGTCGGGCTC	GCTCAGGGTCAGTGTTTCCG
VvGRF8	Vitvi16g04017	GGTCCGGAACCAACCAATCC	AGGCCATGTGCTGTCCAAAG
VvGRF9	Vitvi16g01354	TGTGGTAAGGTGGTGGGTGT	TCCCACGTTGTTGCAGTGTC
VvGRF10	Vitvi18g00623	CTGGTCAGCAAGAACAGCAG	AAGTTCCCAGAGCTGTAACCTGT
VvGIF1	Vitvi07g00563	TGTTCACTTTGCAGTTTGCAGC	ATCAGTGGTGACGTTGCTGG
VvGIF2	Vitvi14g02674	CATTTTGGACCACCACGGTAGA	TGGTATGCTAACTCCACACGC
VvGIF3	Vitvi15g00903	ATGCAGCAGAACCCCCAGAT	GCTGAGCTTGGTACTGTGC
VvGIF4	Vitvi16g01357	CCTTCATTTCCTCCCACCGC	GGTGGTTGGGCATCAGCAAT
GAPDH	F6GSG7_VITVI	CCAAGGCTGGAATAGCACTC	CCATGTGGACAATCAAGTCG

基因ID Gene locus ID	染色体 Chromosome	基因名称 Gene name	起始与终止位点/bp Start and end position	大小/bp Size	CDS/bp	ORF/aa
Vitvi02g00239	2	VvGRF1	2 139 069 ~ 2 141 255	1 986	1 608	535
Vitvi02g00449	2	VvGRF2	4 219 207 ~ 4 220 305	1 098	882	211
Vitvi08g01498	8	VvGRF3	17 729 492 ~ 17 731 764	2 272	1 191	396
Vitvi09g00107	9	VvGRF4	1 134 269 ~ 1 136 573	2 304	1 998	363
Vitvi11g00092	11	VvGRF5	1 000 319 ~ 1 001 707	1 388	1 164	387
Vitvi15g00815	15	VvGRF6	17 227 979 ~ 17 232 031	4 092	1 838	467
Vitvi15g04517	15	VvGRF7	18 612 801 ~ 18 615 995	3 194	1 794	597
Vitvi16g00073	16	VvGRF8	971 916 ~ 975 816	3 900	1 367	330
Vitvi16g01354	16	VvGRF9	22 127 734 ~ 22 130 159	2 425	1 731	576
Vitvi18g00623	18	VvGRF10	7 058 736 ~ 7 061 990	3 254	1 805	599

基因ID Gene locus ID	染色体 Chromosome	基因名称 Gene name	起始与终止位点/bp Start and end position	大小/bp Size	CDS/bp	ORF/aa
Vitvi02g00239	2	VvGRF1	2 139 069 ~ 2 141 255	1 986	1 608	535
Vitvi02g00449	2	VvGRF2	4 219 207 ~ 4 220 305	1 098	882	211
Vitvi08g01498	8	VvGRF3	17 729 492 ~ 17 731 764	2 272	1 191	396
Vitvi09g00107	9	VvGRF4	1 134 269 ~ 1 136 573	2 304	1 998	363
Vitvi11g00092	11	VvGRF5	1 000 319 ~ 1 001 707	1 388	1 164	387
Vitvi15g00815	15	VvGRF6	17 227 979 ~ 17 232 031	4 092	1 838	467
Vitvi15g04517	15	VvGRF7	18 612 801 ~ 18 615 995	3 194	1 794	597
Vitvi16g00073	16	VvGRF8	971 916 ~ 975 816	3 900	1 367	330
Vitvi16g01354	16	VvGRF9	22 127 734 ~ 22 130 159	2 425	1 731	576
Vitvi18g00623	18	VvGRF10	7 058 736 ~ 7 061 990	3 254	1 805	599

基因ID Gene locus ID	染色体 chromosome	基因名称 Gene name	起始与终止位点/bp Start and end position	大小/bp Size	CDS/bp	ORF/aa
Vitvi07g00563	7	VvGIF1	6 086 919 ~ 6 092 810	5 891	1 024	224
Vitvi14g02674	14	VvGIF2	7 536 676 ~ 7 559 301	22 625	1 037	223
Vitvi15g00903	15	VvGIF3	18 236 759 ~ 18 241 761	5 002	1 159	222
Vitvi16g01357	16	VvGIF4	22 146 392 ~ 22 148 829	2 437	597	189