园艺学报 ›› 2023, Vol. 50 ›› Issue (8): 1747-1768.doi: 10.16420/j.issn.0513-353x.2022-0653
收稿日期:
2023-03-01
修回日期:
2023-06-28
出版日期:
2023-08-25
发布日期:
2023-08-23
通讯作者:
作者简介:
* 共同第一作者
基金资助:
LU Yanqing, LIN Yanjin, LU Xinkun**()
Received:
2023-03-01
Revised:
2023-06-28
Published:
2023-08-25
Online:
2023-08-23
摘要:
以‘度尾文旦柚’正常果和果顶部开裂(3个等级)果实为材料,取果顶部的果皮进行转录组测序,筛选到与裂果具有潜在联系的基因。裂果果皮中半乳糖醛酸转移酶(催化聚半乳糖醛酸合成)和阿拉伯半乳聚糖蛋白(维持细胞壁结构)基因表达量显著降低,果胶降解酶类(聚半乳糖醛酸酶、果胶裂解酶)基因表达量显著提高,这些基因表达量的变化可能引起细胞壁机械力度降低,从而导致裂果。3-酮脂酰-CoA合成酶(催化蜡质成分中长链脂肪酸合成的酶)基因在正常果果皮中的表达量是裂果的5倍 ~ 6倍,裂果果皮中该基因表达量的降低可能引起果皮抗蒸腾能力减弱,导致果皮部位水分亏缺,甚至细胞凋亡及裂隙形成。高温和水分亏缺逆境响应基因(编码脱水响应元件结合蛋白、类钙调磷酸酶酶B亚基互作蛋白激酶、WRKY30、WRKY33和WRKY40)在裂果果皮中表达量均显著降低,表明高温和水分亏缺与裂果相关。
卢艳清, 林燕金, 卢新坤. 果皮细胞壁物质代谢及果皮对高温和水分亏缺逆境的响应与‘度尾文旦柚’裂果相关[J]. 园艺学报, 2023, 50(8): 1747-1768.
LU Yanqing, LIN Yanjin, LU Xinkun. Cell Wall Material Metabolism and the Response to High Temperature and Water Deficiency Stresses in Pericarp are Associated with Fruit Cracking of‘Duwei’Pummelo[J]. Acta Horticulturae Sinica, 2023, 50(8): 1747-1768.
图1 ‘度尾文旦柚’正常果(S1)和果皮裂口长度约3、6、10 mm(S2、S3、S4)的果实
Fig. 1 Normal fruit(S1)and cracked fruits with approximate 3,6,and 10 mm of crack length(S2,S3,S4)from‘Duwei’pummelo
基因ID Gene ID | 上游引物(5′-3′) Forward primer | 下游引物(5′-3′) Reverse primer |
---|---|---|
c20641.graph_c0 | GAAAGGCCATTTTGCTGTCTAC | AACCAAACTCTTCCTCAGCTTG |
c24613.graph_c0 | ATGAGAGTGTGGAAGCAGTGAA | CATACTTGACATCCCAGCTGAA |
c38726.graph_c0 | GGTTGCCTTCAAATCTTACTGG | GACATGATTGGTGACAGGATTG |
c27952.graph_c0 | GGAAGAAGGGCATGTTTGTTAC | CCAATAAGTGCTTCTGTGGTGA |
c35493.graph_c0 | GTCTCTCAGCGAGAAATGAGGT | TCCTCATCTAGACGCCGATTAT |
c41357.graph_c0 | AGAGAGGAGCCAAGTGATCAAG | CACGCAGACATCTTTTAGCAAC |
c37766.graph_c1 | TACAAGGAACTTCAGCAACAGC | CTCATTCCCCCAATGTTCTTAC |
c41040.graph_c1 | TATGACGTGATGAAACCTCCAG | TCGTAAACTTCCCTTCTCCGTA |
c24910.graph_c0 | TGGACTAATAGCAGAAGCCACA | AAACCCTAATCCCATGCCTAGT |
c42044.graph_c0 | GCTGCTAGGGTTTGATGACTTT | GACTGCCCTAGCCTACTAAGCA |
c42053.graph_c0 | GCATCGAGAAATGGCTAAAGAC | AGTTTACGCACCTGATCCTTGT |
c41614.graph_c0 | CCCAAGGTTGAATACAGAAAGC | CCCAAGGTTGAATACAGAAAGC |
c30373.graph_c0 | ATGATCAGCGAAGTGGATTCTG | TAAGCTCTTCCTCAGCGTCAGT |
c29562.graph_c0 | TCTTCTACTGGATTGCCCAACT | TGATGATCTCCATCACAACTCC |
Actin | ATGGTTGGTATGGGTCAGAAAG | GCCAGATTTTCTCCATATCGTC |
表1 用于qRT-PCR试验的引物序列
Table 1 Primer sequences in qRT-PCR
基因ID Gene ID | 上游引物(5′-3′) Forward primer | 下游引物(5′-3′) Reverse primer |
---|---|---|
c20641.graph_c0 | GAAAGGCCATTTTGCTGTCTAC | AACCAAACTCTTCCTCAGCTTG |
c24613.graph_c0 | ATGAGAGTGTGGAAGCAGTGAA | CATACTTGACATCCCAGCTGAA |
c38726.graph_c0 | GGTTGCCTTCAAATCTTACTGG | GACATGATTGGTGACAGGATTG |
c27952.graph_c0 | GGAAGAAGGGCATGTTTGTTAC | CCAATAAGTGCTTCTGTGGTGA |
c35493.graph_c0 | GTCTCTCAGCGAGAAATGAGGT | TCCTCATCTAGACGCCGATTAT |
c41357.graph_c0 | AGAGAGGAGCCAAGTGATCAAG | CACGCAGACATCTTTTAGCAAC |
c37766.graph_c1 | TACAAGGAACTTCAGCAACAGC | CTCATTCCCCCAATGTTCTTAC |
c41040.graph_c1 | TATGACGTGATGAAACCTCCAG | TCGTAAACTTCCCTTCTCCGTA |
c24910.graph_c0 | TGGACTAATAGCAGAAGCCACA | AAACCCTAATCCCATGCCTAGT |
c42044.graph_c0 | GCTGCTAGGGTTTGATGACTTT | GACTGCCCTAGCCTACTAAGCA |
c42053.graph_c0 | GCATCGAGAAATGGCTAAAGAC | AGTTTACGCACCTGATCCTTGT |
c41614.graph_c0 | CCCAAGGTTGAATACAGAAAGC | CCCAAGGTTGAATACAGAAAGC |
c30373.graph_c0 | ATGATCAGCGAAGTGGATTCTG | TAAGCTCTTCCTCAGCGTCAGT |
c29562.graph_c0 | TCTTCTACTGGATTGCCCAACT | TGATGATCTCCATCACAACTCC |
Actin | ATGGTTGGTATGGGTCAGAAAG | GCCAGATTTTCTCCATATCGTC |
样品 Sample | Clean read数量 Clean read number | 总碱基数 Base number | GC碱基含量/% GC percentage | 碱基质量Q30/% Base quality Q30 |
---|---|---|---|---|
S1-1 | 26 308 889 | 7 821 704 554 | 44.39 | 94.93 |
S1-2 | 24 595 318 | 7 338 467 348 | 44.39 | 94.80 |
S1-3 | 27 460 893 | 8 175 872 264 | 44.49 | 94.66 |
S2-1 | 29 498 250 | 8 816 423 378 | 44.69 | 94.45 |
S2-2 | 30 876 130 | 9 205 400 330 | 45.44 | 93.15 |
S2-3 | 30 073 009 | 8 982 592 242 | 45.20 | 94.77 |
S3-1 | 30 052 073 | 8 966 732 468 | 44.45 | 94.93 |
S3-2 | 30 586 330 | 9 105 437 120 | 44.71 | 94.97 |
S3-3 | 32 742 522 | 9 746 030 622 | 45.23 | 95.04 |
S4-1 | 23 597 373 | 7 056 930 884 | 44.57 | 94.69 |
S4-2 | 28 327 712 | 8 459 873 358 | 44.37 | 94.52 |
S4-3 | 29 249 465 | 8 738 373 420 | 44.62 | 94.61 |
表2 ‘度尾文旦柚’正常果和裂果果皮cDNA文库测序数据分析
Table 2 Statistical analysis of cDNA libraries from the normal and cracked pericarp of‘Duwei’pummelo fruits
样品 Sample | Clean read数量 Clean read number | 总碱基数 Base number | GC碱基含量/% GC percentage | 碱基质量Q30/% Base quality Q30 |
---|---|---|---|---|
S1-1 | 26 308 889 | 7 821 704 554 | 44.39 | 94.93 |
S1-2 | 24 595 318 | 7 338 467 348 | 44.39 | 94.80 |
S1-3 | 27 460 893 | 8 175 872 264 | 44.49 | 94.66 |
S2-1 | 29 498 250 | 8 816 423 378 | 44.69 | 94.45 |
S2-2 | 30 876 130 | 9 205 400 330 | 45.44 | 93.15 |
S2-3 | 30 073 009 | 8 982 592 242 | 45.20 | 94.77 |
S3-1 | 30 052 073 | 8 966 732 468 | 44.45 | 94.93 |
S3-2 | 30 586 330 | 9 105 437 120 | 44.71 | 94.97 |
S3-3 | 32 742 522 | 9 746 030 622 | 45.23 | 95.04 |
S4-1 | 23 597 373 | 7 056 930 884 | 44.57 | 94.69 |
S4-2 | 28 327 712 | 8 459 873 358 | 44.37 | 94.52 |
S4-3 | 29 249 465 | 8 738 373 420 | 44.62 | 94.61 |
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c38726.graph_c0 | 34.31 | 112.21 | 76.09 | 139.07 | 2.93E-08 | 2.64E-104 | 3.53E-05 | 苯丙氨酸解氨酶 Phenylalanine ammonia-lyase |
c41125.graph_c1 | 77.88 | 28.68 | — | 35.25 | 4.66E-27 | — | 2.26E-36 | 类肉桂酰辅酶A还原酶SNL6 Cinnamoyl-CoA reductase-like SNL6 |
c39674.graph_c2 | 33.86 | 73.54 | 74.09 | — | 1.35E-13 | 7.41E-82 | — | 4-香豆酸-CoA连接酶1 4-Coumarate-CoA ligase 1 |
c38889.graph_c4 | 26.43 | 65.44 | — | 76.18 | 3.32E-22 | — | 7.24E-102 | 莽草酸羟基肉桂酰基辅酶A转移酶 OShikimate O-hydroxycinnamoyltransferase |
c39572.graph_c0 | 0.18 | 0.93 | — | — | 4.19E-03 | — | — | 阿魏酰辅酶A邻羟基酶1 Feruloyl CoA ortho-hydroxylase 1 |
c36288.graph_c0 | 67.89 | 30.82 | — | — | 1.30E-12 | — | — | 类纤维素合成酶蛋白D2 Cellulose synthase-like protein D2 |
c36288.graph_c1 | 139.04 | 71.66 | — | — | 2.35E-41 | — | — | 类纤维素合成酶蛋白D2 Cellulose synthase-like protein D2 |
c34939.graph_c0 | 0.11 | 0.61 | — | — | 9.13E-03 | — | — | 类纤维素合成酶蛋白D5 Cellulose synthase-like protein D5 |
c32442.graph_c1 | 0.04 | 2.47 | — | 10.59 | 7.08E-16 | — | 1.21E-85 | 内切葡聚糖酶1 Endoglucanase 1 |
c24613.graph_c0 | 4.61 | 10.20 | — | — | 1.51E-09 | — | — | 内切葡聚糖酶11 Endoglucanase 11 |
c22695.graph_c0 | 2.85 | 0.18 | — | — | 8.40E-03 | — | — | 木葡聚糖内切葡聚糖酶/水解酶类似蛋白25 Probable xyloglucan endotransglu- cosylase/hydrolase protein 25 |
c35921.graph_c0 | 79.96 | 6.12 | — | 24.95 | 7.69E-86 | — | 1.13E-44 | 木葡聚糖内切葡聚糖酶/水解酶类似蛋白25 Probable xyloglucan endotransglu- cosylase/hydrolase protein 25 |
c24668.graph_c0 | 0.86 | 3.64 | 2.65 | 9.09 | 5.56E-06 | 4.55E-09 | 3.75E-42 | 类木葡聚糖半乳糖转移酶GT14 Probable xyloglucan galactosyltransferase GT14 |
c41958.graph_c0 | 69.47 | 141.03 | — | — | 3.15E-22 | — | — | 聚半乳糖醛酸酶1β类似蛋白3 Polygalacturonase 1 beta-like protein 3 |
c36165.graph_c0 | 20.69 | 6.46 | — | — | 3.27E-33 | — | — | 类半乳糖醛酸转移酶9 Probable galacturonosyltransferase-like 9 |
c30366.graph_c0 | 128.71 | 22.25 | — | 64.70 | 2.30E-17 | — | 1.37E-30 | 类半乳糖醛酸转移酶10 Probable galacturonosyltransferase-like 10 |
c23503.graph_c1 | 9.78 | 27.02 | — | — | 2.73E-09 | — | — | 果胶裂解酶11 Putative pectate lyase 11 |
c21544.graph_c0 | 0.39 | 1.58 | — | 1.82 | 4.23E-04 | — | 3.41E-06 | 果胶裂解酶P18 Probable pectate lyase P18 |
c32944.graph_c0 | 0.89 | 3.86 | — | — | 2.19E-05 | — | — | 果胶甲酯酶抑制因子10 Pectinesterase inhibitor 10 |
c38505.graph_c0 | 122.47 | 27.75 | — | 53.90 | 2.00E-73 | — | 3.14E-31 | 阿拉伯半乳聚糖蛋白16 Arabinogalactan protein 16 |
表3 度尾文旦柚正常果和裂果果皮细胞壁物质代谢途径相关差异基因表达量的变化和功能注释
Table 3 Changes in expression levels and functional annotation of differentially expressed genes involved in cell wall material metabolic pathway in normal and cracked pericarp from‘Duwei’pummelo fruits
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c38726.graph_c0 | 34.31 | 112.21 | 76.09 | 139.07 | 2.93E-08 | 2.64E-104 | 3.53E-05 | 苯丙氨酸解氨酶 Phenylalanine ammonia-lyase |
c41125.graph_c1 | 77.88 | 28.68 | — | 35.25 | 4.66E-27 | — | 2.26E-36 | 类肉桂酰辅酶A还原酶SNL6 Cinnamoyl-CoA reductase-like SNL6 |
c39674.graph_c2 | 33.86 | 73.54 | 74.09 | — | 1.35E-13 | 7.41E-82 | — | 4-香豆酸-CoA连接酶1 4-Coumarate-CoA ligase 1 |
c38889.graph_c4 | 26.43 | 65.44 | — | 76.18 | 3.32E-22 | — | 7.24E-102 | 莽草酸羟基肉桂酰基辅酶A转移酶 OShikimate O-hydroxycinnamoyltransferase |
c39572.graph_c0 | 0.18 | 0.93 | — | — | 4.19E-03 | — | — | 阿魏酰辅酶A邻羟基酶1 Feruloyl CoA ortho-hydroxylase 1 |
c36288.graph_c0 | 67.89 | 30.82 | — | — | 1.30E-12 | — | — | 类纤维素合成酶蛋白D2 Cellulose synthase-like protein D2 |
c36288.graph_c1 | 139.04 | 71.66 | — | — | 2.35E-41 | — | — | 类纤维素合成酶蛋白D2 Cellulose synthase-like protein D2 |
c34939.graph_c0 | 0.11 | 0.61 | — | — | 9.13E-03 | — | — | 类纤维素合成酶蛋白D5 Cellulose synthase-like protein D5 |
c32442.graph_c1 | 0.04 | 2.47 | — | 10.59 | 7.08E-16 | — | 1.21E-85 | 内切葡聚糖酶1 Endoglucanase 1 |
c24613.graph_c0 | 4.61 | 10.20 | — | — | 1.51E-09 | — | — | 内切葡聚糖酶11 Endoglucanase 11 |
c22695.graph_c0 | 2.85 | 0.18 | — | — | 8.40E-03 | — | — | 木葡聚糖内切葡聚糖酶/水解酶类似蛋白25 Probable xyloglucan endotransglu- cosylase/hydrolase protein 25 |
c35921.graph_c0 | 79.96 | 6.12 | — | 24.95 | 7.69E-86 | — | 1.13E-44 | 木葡聚糖内切葡聚糖酶/水解酶类似蛋白25 Probable xyloglucan endotransglu- cosylase/hydrolase protein 25 |
c24668.graph_c0 | 0.86 | 3.64 | 2.65 | 9.09 | 5.56E-06 | 4.55E-09 | 3.75E-42 | 类木葡聚糖半乳糖转移酶GT14 Probable xyloglucan galactosyltransferase GT14 |
c41958.graph_c0 | 69.47 | 141.03 | — | — | 3.15E-22 | — | — | 聚半乳糖醛酸酶1β类似蛋白3 Polygalacturonase 1 beta-like protein 3 |
c36165.graph_c0 | 20.69 | 6.46 | — | — | 3.27E-33 | — | — | 类半乳糖醛酸转移酶9 Probable galacturonosyltransferase-like 9 |
c30366.graph_c0 | 128.71 | 22.25 | — | 64.70 | 2.30E-17 | — | 1.37E-30 | 类半乳糖醛酸转移酶10 Probable galacturonosyltransferase-like 10 |
c23503.graph_c1 | 9.78 | 27.02 | — | — | 2.73E-09 | — | — | 果胶裂解酶11 Putative pectate lyase 11 |
c21544.graph_c0 | 0.39 | 1.58 | — | 1.82 | 4.23E-04 | — | 3.41E-06 | 果胶裂解酶P18 Probable pectate lyase P18 |
c32944.graph_c0 | 0.89 | 3.86 | — | — | 2.19E-05 | — | — | 果胶甲酯酶抑制因子10 Pectinesterase inhibitor 10 |
c38505.graph_c0 | 122.47 | 27.75 | — | 53.90 | 2.00E-73 | — | 3.14E-31 | 阿拉伯半乳聚糖蛋白16 Arabinogalactan protein 16 |
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c41238.graph_c0 | 57.39 | 25.67 | — | — | 8.31E-35 | — | — | δ(8)-脂肪酸去饱和酶2 Delta (8)-fatty-acid desaturase 2 |
c41838.graph_c0 | 84.61 | 16.50 | — | 27.34 | 2.69E-144 | — | 6.31E-98 | 3-酮脂酰-CoA合成酶 3-ketoacyl-CoA synthase |
c42251.graph_c0 | 15.67 | 2.44 | — | 5.25 | 1.37E-11 | — | 3.37E-06 | 3-酮脂酰-CoA合成酶 3-ketoacyl-CoA synthase |
表4 度尾文旦柚正常果和裂果果皮脂质代谢途径相关差异基因表达量的变化和功能注释
Table 4 Changes in expression levels and functional annotation of differentially expressed genes involved in lipid metabolic pathway in normal and cracked pericarp from‘Duwei’pummelo fruits
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c41238.graph_c0 | 57.39 | 25.67 | — | — | 8.31E-35 | — | — | δ(8)-脂肪酸去饱和酶2 Delta (8)-fatty-acid desaturase 2 |
c41838.graph_c0 | 84.61 | 16.50 | — | 27.34 | 2.69E-144 | — | 6.31E-98 | 3-酮脂酰-CoA合成酶 3-ketoacyl-CoA synthase |
c42251.graph_c0 | 15.67 | 2.44 | — | 5.25 | 1.37E-11 | — | 3.37E-06 | 3-酮脂酰-CoA合成酶 3-ketoacyl-CoA synthase |
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c36886.graph_c0 | 21.55 | 52.93 | 40.10 | — | 9.69E-37 | 3.57E-56 | — | 类ERD6糖转运蛋白16 Sugar transporter ERD6-like 16 |
c41975.graph_c0 | 0.07 | 2.79 | — | 1.65E-05 | — | — | 非特异性脂质运输蛋白8 Non-specific lipid-transfer protein 8 | |
c29562.graph_c0 | 0.75 | 3.48 | 2.55 | — | 3.53E-04 | 1.25E-05 | — | 水通道蛋白TIP2-1 Aquaporin TIP2-1 |
c38825.graph_c0 | 2.61 | 8.62 | 5.61 | 20.31 | 1.62E-11 | 1.00E-11 | 2.99E-10 | 钾离子运输载体5 Potassium transporter 5 |
c31401.graph_c0 | 9.00 | 2.06 | — | — | 3.44E-49 | — | — | 钙离子运输ATP酶12 Calcium-transporting ATPase 12 |
表5 度尾文旦柚正常果和裂果果皮物质运输途径相关差异基因表达量的变化和功能注释
Table 5 Changes in expression levels and functional annotation of differentially expressed genes involved in substance transport pathway in normal and cracked pericarp from‘Duwei’pummelo fruits
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c36886.graph_c0 | 21.55 | 52.93 | 40.10 | — | 9.69E-37 | 3.57E-56 | — | 类ERD6糖转运蛋白16 Sugar transporter ERD6-like 16 |
c41975.graph_c0 | 0.07 | 2.79 | — | 1.65E-05 | — | — | 非特异性脂质运输蛋白8 Non-specific lipid-transfer protein 8 | |
c29562.graph_c0 | 0.75 | 3.48 | 2.55 | — | 3.53E-04 | 1.25E-05 | — | 水通道蛋白TIP2-1 Aquaporin TIP2-1 |
c38825.graph_c0 | 2.61 | 8.62 | 5.61 | 20.31 | 1.62E-11 | 1.00E-11 | 2.99E-10 | 钾离子运输载体5 Potassium transporter 5 |
c31401.graph_c0 | 9.00 | 2.06 | — | — | 3.44E-49 | — | — | 钙离子运输ATP酶12 Calcium-transporting ATPase 12 |
基因号 | FPKM | FDR | 功能注释 | |||||
---|---|---|---|---|---|---|---|---|
Gene number | S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | Functional annotation |
c27806.graph_c0 | 18.95 | 8.79 | — | — | 1.88E-09 | — | — | 谷胱甘肽S-转移酶 Probable glutathione S-transferase |
c27881.graph_c0 | 46.03 | 9.52 | — | — | 2.04E-87 | — | — | L-抗坏血酸氧化酶 L-ascorbate oxidase |
c41040.graph_c1 | 95.24 | 212.16 | — | — | 2.44E-16 | — | — | L-抗坏血酸氧化酶 L-ascorbate oxidase |
c33328.graph_c0 | 2.04 | 4.48 | — | — | 4.64E-06 | — | — | 过氧化物酶30 Peroxidase 30 |
c27794.graph_c0 | 0.19 | 1.46 | — | — | 8.16E-06 | — | — | 过氧化物酶65 Peroxidase 65 |
c39811.graph_c1 | 0.53 | 4.71 | — | 44.87 | 2.60E-08 | — | 4.02E-106 | 过氧化物酶73 Peroxidase 73 |
c28308.graph_c0 | 0.61 | 2.42 | 2.24 | 3.93 | 6.49E-05 | 4.48E-06 | 4.72E-13 | 过氧化物酶73 Peroxidase 73 |
c36437.graph_c0 | 4.98 | 11.28 | — | 10.85 | 5.56E-11 | — | 5.33E-09 | 过氧化物酶P7 Peroxidase P7 |
c24516.graph_c0 | 0 | 6.93 | — | 76.95 | 7.13E-06 | — | 8.72E-83 | 阳离子过氧化物酶1 Cationic peroxidase 1 |
c35137.graph_c0 | 3.26 | 10.53 | — | — | 1.26E-20 | — | — | 细胞色素P450 78A6 Cytochrome P450 78A6 |
c26281.graph_c0 | 0 | 0.46 | — | — | 7.54E-04 | — | — | 细胞色素P450 86A1 Cytochrome P450 86A1 |
c31473.graph_c1 | 3.97 | 8.72 | — | — | 2.98E-12 | — | — | 细胞色素P450 86A8 Cytochrome P450 86A8 |
c41978.graph_c0 | 4.71 | 15.33 | 9.94 | 21.49 | 2.10E-03 | 1.54E-12 | 1.16E-05 | 细胞色素P450 83B1 Cytochrome P450 83B1 |
c44951.graph_c0 | 0 | 0.46 | — | — | 1.58E-03 | — | — | 细胞色素P450 94B3 Cytochrome P450 94B3 |
c41248.graph_c1 | 44.91 | 101.72 | — | — | 1.40E-25 | — | — | 细胞色素P450 82C4 Cytochrome P450 82C4 |
c38532.graph_c0 | 37.50 | 11.82 | — | 14.48 | 3.09E-42 | — | 1.64E-49 | 细胞色素P450 94C1 Cytochrome P450 94C1 |
表6 度尾文旦柚正常果和裂果果皮参与氧化还原反应的相关差异基因表达量的变化和功能注释
Table 6 Changes in expression levels and functional annotation of differentially expressed genes involved in oxidation-reduction reaction in normal and cracked pericarp from‘Duwei’pummelo fruits
基因号 | FPKM | FDR | 功能注释 | |||||
---|---|---|---|---|---|---|---|---|
Gene number | S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | Functional annotation |
c27806.graph_c0 | 18.95 | 8.79 | — | — | 1.88E-09 | — | — | 谷胱甘肽S-转移酶 Probable glutathione S-transferase |
c27881.graph_c0 | 46.03 | 9.52 | — | — | 2.04E-87 | — | — | L-抗坏血酸氧化酶 L-ascorbate oxidase |
c41040.graph_c1 | 95.24 | 212.16 | — | — | 2.44E-16 | — | — | L-抗坏血酸氧化酶 L-ascorbate oxidase |
c33328.graph_c0 | 2.04 | 4.48 | — | — | 4.64E-06 | — | — | 过氧化物酶30 Peroxidase 30 |
c27794.graph_c0 | 0.19 | 1.46 | — | — | 8.16E-06 | — | — | 过氧化物酶65 Peroxidase 65 |
c39811.graph_c1 | 0.53 | 4.71 | — | 44.87 | 2.60E-08 | — | 4.02E-106 | 过氧化物酶73 Peroxidase 73 |
c28308.graph_c0 | 0.61 | 2.42 | 2.24 | 3.93 | 6.49E-05 | 4.48E-06 | 4.72E-13 | 过氧化物酶73 Peroxidase 73 |
c36437.graph_c0 | 4.98 | 11.28 | — | 10.85 | 5.56E-11 | — | 5.33E-09 | 过氧化物酶P7 Peroxidase P7 |
c24516.graph_c0 | 0 | 6.93 | — | 76.95 | 7.13E-06 | — | 8.72E-83 | 阳离子过氧化物酶1 Cationic peroxidase 1 |
c35137.graph_c0 | 3.26 | 10.53 | — | — | 1.26E-20 | — | — | 细胞色素P450 78A6 Cytochrome P450 78A6 |
c26281.graph_c0 | 0 | 0.46 | — | — | 7.54E-04 | — | — | 细胞色素P450 86A1 Cytochrome P450 86A1 |
c31473.graph_c1 | 3.97 | 8.72 | — | — | 2.98E-12 | — | — | 细胞色素P450 86A8 Cytochrome P450 86A8 |
c41978.graph_c0 | 4.71 | 15.33 | 9.94 | 21.49 | 2.10E-03 | 1.54E-12 | 1.16E-05 | 细胞色素P450 83B1 Cytochrome P450 83B1 |
c44951.graph_c0 | 0 | 0.46 | — | — | 1.58E-03 | — | — | 细胞色素P450 94B3 Cytochrome P450 94B3 |
c41248.graph_c1 | 44.91 | 101.72 | — | — | 1.40E-25 | — | — | 细胞色素P450 82C4 Cytochrome P450 82C4 |
c38532.graph_c0 | 37.50 | 11.82 | — | 14.48 | 3.09E-42 | — | 1.64E-49 | 细胞色素P450 94C1 Cytochrome P450 94C1 |
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c31925.graph_c0 | 3.96 | 12.00 | 24.04 | 31.57 | 3.46E-13 | 7.13E-117 | 1.49E-03 | 1-氨基环丙烷-1-羧酸合成酶1 1-aminocyclopropane-1-carboxylate synthase 1 |
c34661.graph_c1 | 11.83 | 28.35 | — | 42.14 | 2.60E-13 | — | 4.58E-16 | 乙烯响应转录因子2 Ethylene-responsive transcription factor 2 |
c24910.graph_c0 | 18.01 | 7.56 | — | — | 2.61E-09 | — | — | 乙烯响应转录因子1B Ethylene-responsive transcription factor 1B |
c22110.graph_c0 | 21.84 | 9.22 | — | 8.06 | 1.01E-06 | — | 2.97E-17 | 乙烯响应转录因子ERF003 Ethylene-responsive transcription factor ERF003 |
c33092.graph_c0 | 67.92 | 27.43 | — | 32.51 | 1.17E-17 | — | 6.18E-34 | 乙烯响应转录因子6 Ethylene-responsive transcription factor 6 |
c41869.graph_c0 | 83.76 | 21.24 | — | 34.08 | 3.92E-34 | — | 9.71E-26 | 乙烯响应转录因子ERF012 Ethylene-responsive transcription factor ERF012 |
c30725.graph_c0 | 20.40 | 3.39 | — | — | 9.40E-25 | — | — | 乙烯响应转录因子ERF109 Ethylene-responsive transcription factor ERF109 |
c20641.graph_c0 | 1.54 | 4.92 | — | 3.92 | 3.56E-05 | — | 7.79E-04 | 生长素响应蛋白SAUR50 Auxin-responsive protein SAUR50 |
c27505.graph_c0 | 17.46 | 4.68 | — | — | 6.72E-25 | — | — | 生长素响应蛋白IAA29 Auxin-responsive protein IAA29 |
表7 度尾文旦柚正常果和裂果果皮植物激素代谢及其信号转导途径相关差异基因表达量的变化和功能注释
Table 7 Changes in expression levels and functional annotation of differentially expressed genes involved in plant hormone metabolic pathways and plant hormone signaling pathways in normal and cracked pericarp from‘Duwei’pummelo fruits
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c31925.graph_c0 | 3.96 | 12.00 | 24.04 | 31.57 | 3.46E-13 | 7.13E-117 | 1.49E-03 | 1-氨基环丙烷-1-羧酸合成酶1 1-aminocyclopropane-1-carboxylate synthase 1 |
c34661.graph_c1 | 11.83 | 28.35 | — | 42.14 | 2.60E-13 | — | 4.58E-16 | 乙烯响应转录因子2 Ethylene-responsive transcription factor 2 |
c24910.graph_c0 | 18.01 | 7.56 | — | — | 2.61E-09 | — | — | 乙烯响应转录因子1B Ethylene-responsive transcription factor 1B |
c22110.graph_c0 | 21.84 | 9.22 | — | 8.06 | 1.01E-06 | — | 2.97E-17 | 乙烯响应转录因子ERF003 Ethylene-responsive transcription factor ERF003 |
c33092.graph_c0 | 67.92 | 27.43 | — | 32.51 | 1.17E-17 | — | 6.18E-34 | 乙烯响应转录因子6 Ethylene-responsive transcription factor 6 |
c41869.graph_c0 | 83.76 | 21.24 | — | 34.08 | 3.92E-34 | — | 9.71E-26 | 乙烯响应转录因子ERF012 Ethylene-responsive transcription factor ERF012 |
c30725.graph_c0 | 20.40 | 3.39 | — | — | 9.40E-25 | — | — | 乙烯响应转录因子ERF109 Ethylene-responsive transcription factor ERF109 |
c20641.graph_c0 | 1.54 | 4.92 | — | 3.92 | 3.56E-05 | — | 7.79E-04 | 生长素响应蛋白SAUR50 Auxin-responsive protein SAUR50 |
c27505.graph_c0 | 17.46 | 4.68 | — | — | 6.72E-25 | — | — | 生长素响应蛋白IAA29 Auxin-responsive protein IAA29 |
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c31950.graph_c0 | 120.36 | 56.66 | — | — | 9.25E-21 | — | — | 类钙调磷酸酶B亚基互作蛋白激酶5 CBL-interacting protein kinase 5 |
c35904.graph_c2 | 85.01 | 41.21 | — | — | 2.68E-17 | — | — | 钙调素结合蛋白25 Calmodulin-binding protein 25 |
c32041.graph_c0 | 36.24 | 15.37 | — | — | 2.41E-28 | — | — | 钙调素结合蛋白60 B Calmodulin-binding protein 60 B |
c36358.graph_c1 | 123.02 | 44.81 | — | 52.89 | 6.78E-71 | — | 9.95E-46 | 钙调素结合蛋白60 E Calmodulin-binding protein 60 E |
c39881.graph_c0 | 320.87 | 140.00 | — | — | 1.78E-56 | — | — | WRKY 30转录因子 Probable WRKY transcription factor 30 |
c36727.graph_c0 | 59.98 | 12.61 | — | 27.91 | 6.23E-64 | — | 2.57E-16 | WRKY 33转录因子 Probable WRKY transcription factor 33 |
c30233.graph_c0 | 55.73 | 14.41 | — | 23.07 | 1.53E-66 | — | 5.63E-16 | WRKY 40转录因子 Probable WRKY transcription factor 40 |
c33780.graph_c0 | 201.50 | 73.90 | — | — | 1.09E-51 | — | — | WRKY 40转录因子 Probable WRKY transcription factor 40 |
c23445.graph_c0 | 104.01 | 29.37 | — | 40.42 | 2.67E-58 | — | 2.87E-44 | 脱水响应元件结合蛋白1D Dehydration-responsive element-binding protein 1D |
表8 度尾文旦柚正常果和裂果果皮高温响应基因表达量的变化和功能注释
Table 8 Changes in expression levels and functional annotation of genes response to high temperature in normal and cracked pericarp from‘Duwei’pummelo fruits
基因号 Gene number | FPKM | FDR | 功能注释 Functional annotation | |||||
---|---|---|---|---|---|---|---|---|
S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | ||
c31950.graph_c0 | 120.36 | 56.66 | — | — | 9.25E-21 | — | — | 类钙调磷酸酶B亚基互作蛋白激酶5 CBL-interacting protein kinase 5 |
c35904.graph_c2 | 85.01 | 41.21 | — | — | 2.68E-17 | — | — | 钙调素结合蛋白25 Calmodulin-binding protein 25 |
c32041.graph_c0 | 36.24 | 15.37 | — | — | 2.41E-28 | — | — | 钙调素结合蛋白60 B Calmodulin-binding protein 60 B |
c36358.graph_c1 | 123.02 | 44.81 | — | 52.89 | 6.78E-71 | — | 9.95E-46 | 钙调素结合蛋白60 E Calmodulin-binding protein 60 E |
c39881.graph_c0 | 320.87 | 140.00 | — | — | 1.78E-56 | — | — | WRKY 30转录因子 Probable WRKY transcription factor 30 |
c36727.graph_c0 | 59.98 | 12.61 | — | 27.91 | 6.23E-64 | — | 2.57E-16 | WRKY 33转录因子 Probable WRKY transcription factor 33 |
c30233.graph_c0 | 55.73 | 14.41 | — | 23.07 | 1.53E-66 | — | 5.63E-16 | WRKY 40转录因子 Probable WRKY transcription factor 40 |
c33780.graph_c0 | 201.50 | 73.90 | — | — | 1.09E-51 | — | — | WRKY 40转录因子 Probable WRKY transcription factor 40 |
c23445.graph_c0 | 104.01 | 29.37 | — | 40.42 | 2.67E-58 | — | 2.87E-44 | 脱水响应元件结合蛋白1D Dehydration-responsive element-binding protein 1D |
基因号 | FPKM | FDR | 功能注释 | |||||
---|---|---|---|---|---|---|---|---|
Gene number | S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | Functional annotation |
c34657.graph_c0 | 4.61 | 2.02 | — | — | 1.38E-04 | — | — | LRR受体激酶BAK1 LRR receptor kinase BAK1 |
c39343.graph_c0 | 4.90 | 2.21 | — | — | 4.61E-06 | — | — | LRR类受体丝氨酸/苏氨酸蛋白激酶 Probable LRR receptor-like serine/threonine-protein kinase |
c41499.graph_c0 | 30.54 | 11.11 | — | — | 6.68E-51 | — | — | LRR类受体丝氨酸/苏氨酸蛋白激酶 Probable LRR receptor-like serine/threonine-protein kinase |
表9 度尾文旦柚正常果和裂果果皮光照响应基因表达量的变化和功能注释
Table 9 Changes in expression levels and functional annotation of genes response to light in normal and cracked pericarp From‘Duwei’pummelo fruits
基因号 | FPKM | FDR | 功能注释 | |||||
---|---|---|---|---|---|---|---|---|
Gene number | S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | Functional annotation |
c34657.graph_c0 | 4.61 | 2.02 | — | — | 1.38E-04 | — | — | LRR受体激酶BAK1 LRR receptor kinase BAK1 |
c39343.graph_c0 | 4.90 | 2.21 | — | — | 4.61E-06 | — | — | LRR类受体丝氨酸/苏氨酸蛋白激酶 Probable LRR receptor-like serine/threonine-protein kinase |
c41499.graph_c0 | 30.54 | 11.11 | — | — | 6.68E-51 | — | — | LRR类受体丝氨酸/苏氨酸蛋白激酶 Probable LRR receptor-like serine/threonine-protein kinase |
基因号 | FPKM | FDR | 功能注释 | |||||
---|---|---|---|---|---|---|---|---|
Gene number | S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | Functional annotation |
c41451.graph_c0 | 290.39 | 86.72 | — | 141.35 | 2.32E-94 | — | 1.24E-54 | 丝裂原活化蛋白激酶3 Mitogen-activated protein kinase 3 |
c42328.graph_c0 | 98.24 | 30.38 | — | — | 2.62E-60 | — | — | 丝裂原活化蛋白激酶激酶激酶17 Mitogen-activated protein kinase kinase kinase 17 |
c30633.graph_c0 | 1.74 | 0.55 | — | — | 3.21E-03 | — | — | 丝裂原活化蛋白激酶激酶激酶17 Mitogen-activated protein kinase kinase kinase 17 |
c27988.graph_c0 | 15.42 | 3.15 | 29.35 | — | 6.45E-49 | 2.74E-40 | — | 丝裂原活化蛋白激酶激酶激酶18 Mitogen-activated protein kinase kinase kinase 18 |
c29755.graph_c0 | 6.95 | 1.98 | — | — | 1.93E-15 | — | — | 丝裂原活化蛋白激酶激酶激酶18 Mitogen-activated protein kinase kinase kinase 18 |
c22904.graph_c0 | 52.08 | 21.77 | — | — | 2.22E-44 | — | — | 类受体蛋白激酶HERK 1 Receptor-like protein kinase HERK 1 |
c41886.graph_c0 | 7.22 | 3.04 | — | — | 2.41E-06 | — | — | 类受体蛋白激酶 Probable receptor-like protein kinase |
c35877.graph_c1 | 6.15 | 1.88 | — | 1.71 | 5.74E-04 | — | 4.46E-06 | 富含半胱氨酸的类受体蛋白激酶20 Putative cysteine-rich receptor-like protein kinase 20 |
c34166.graph_c0 | 11.39 | 2.47 | — | — | 2.19E-32 | — | — | 丝氨酸/苏氨酸蛋白激酶类蛋白 CCR3 Putative serine/threonine-protein kinase-like protein CCR3 |
c33028.graph_c0 | 29.69 | 15.71 | — | — | 4.17E-24 | — | — | 丝氨酸/苏氨酸蛋白激酶PBL5 Probable serine/threonine-protein kinase PBL5 |
c33975.graph_c0 | 2.62 | 8.77 | — | 9.34 | 2.72E-07 | — | 2.25E-08 | 丝氨酸/苏氨酸蛋白激酶PIX13 Probable serine/threonine-protein kinase PIX13 |
c40710.graph_c0 | 17.50 | 8.15 | — | — | 3.24E-23 | — | — | 丝氨酸/苏氨酸蛋白激酶PBL3 Probable serine/threonine-protein kinase PBL3 |
c32569.graph_c0 | 4.53 | 11.26 | 10.70 | — | 1.63E-09 | 6.70E-21 | — | 类受体丝氨酸/苏氨酸蛋白激酶 Receptor-like serine/threonine- protein kinase |
c40773.graph_c2 | 200.52 | 48.05 | — | — | 1.71E-55 | — | — | 蛋白磷酸酶2C 25 Probable protein phosphatase 2C 25 |
c37271.graph_c0 | 25.40 | 7.32 | — | — | 1.12E-37 | — | — | 蛋白磷酸酶2C 30 Probable protein phosphatase 2C 30 |
c42044.graph_c0 | 1.49 | 0.32 | 5.29 | — | 4.45E-03 | 6.16E-10 | — | 钙结合蛋白CML31 Probable calcium-binding protein CML31 |
c32527.graph_c0 | 63.33 | 13.99 | — | 29.97 | 3.84E-53 | — | 3.08E-18 | 钙结合蛋白CML37 Calcium-binding protein CML37 |
c34433.graph_c1 | 153.82 | 61.44 | — | — | 3.46E-22 | — | — | 钙结合蛋白CML45 Probable calcium-binding protein CML45 |
c24793.graph_c0 | 29.94 | 12.63 | — | 14.07 | 3.13E-15 | — | 1.35E-07 | MYB4转录因子 Transcription factor MYB4 |
c26406.graph_c0 | 167.49 | 57.65 | — | — | 1.82E-45 | — | — | MYB4转录因子 Transcription factor MYB4 |
c35593.graph_c0 | 93.03 | 43.27 | — | — | 1.13E-27 | — | — | MYB44转录因子 Transcription factor MYB44 |
c41858.graph_c0 | 224.63 | 40.70 | — | — | 3.66E-76 | — | — | 锌指蛋白ZAT8 Zinc finger protein ZAT8 |
c23865.graph_c0 | 256.34 | 95.74 | — | 102.04 | 2.35E-43 | — | 1.07E-42 | 锌指蛋白ZAT10 Zinc finger protein ZAT10 |
c28537.graph_c0 | 264.63 | 82.51 | — | — | 3.23E-32 | — | — | 锌指蛋白ZAT10 Zinc finger protein ZAT10 |
c28249.graph_c0 | 20.67 | 3.85 | — | — | 4.70E-30 | — | — | 锌指蛋白ZAT11 Zinc finger protein ZAT11 |
c32466.graph_c0 | 164.66 | 48.15 | — | — | 2.55E-74 | — | — | RING-H2锌指蛋白ATL2 RING-H2 finger protein ATL2 |
c38319.graph_c3 | 53.94 | 16.60 | — | 26.76 | 1.79E-41 | — | 8.40E-28 | RING-H2锌指蛋白 ATL60 RING-H2 finger protein ATL60 |
表10 度尾文旦柚正常果和裂果果皮水分亏缺逆境响应基因表达量的变化和功能注释
Table 10 Changes in expression levels and functional annotation of genes response to water deficiency in normal and cracked pericarp from‘Duwei’pummelo fruits
基因号 | FPKM | FDR | 功能注释 | |||||
---|---|---|---|---|---|---|---|---|
Gene number | S1 | S2 | S3 | S4 | S1 vs. S2 | S1 vs. S3 | S1 vs. S4 | Functional annotation |
c41451.graph_c0 | 290.39 | 86.72 | — | 141.35 | 2.32E-94 | — | 1.24E-54 | 丝裂原活化蛋白激酶3 Mitogen-activated protein kinase 3 |
c42328.graph_c0 | 98.24 | 30.38 | — | — | 2.62E-60 | — | — | 丝裂原活化蛋白激酶激酶激酶17 Mitogen-activated protein kinase kinase kinase 17 |
c30633.graph_c0 | 1.74 | 0.55 | — | — | 3.21E-03 | — | — | 丝裂原活化蛋白激酶激酶激酶17 Mitogen-activated protein kinase kinase kinase 17 |
c27988.graph_c0 | 15.42 | 3.15 | 29.35 | — | 6.45E-49 | 2.74E-40 | — | 丝裂原活化蛋白激酶激酶激酶18 Mitogen-activated protein kinase kinase kinase 18 |
c29755.graph_c0 | 6.95 | 1.98 | — | — | 1.93E-15 | — | — | 丝裂原活化蛋白激酶激酶激酶18 Mitogen-activated protein kinase kinase kinase 18 |
c22904.graph_c0 | 52.08 | 21.77 | — | — | 2.22E-44 | — | — | 类受体蛋白激酶HERK 1 Receptor-like protein kinase HERK 1 |
c41886.graph_c0 | 7.22 | 3.04 | — | — | 2.41E-06 | — | — | 类受体蛋白激酶 Probable receptor-like protein kinase |
c35877.graph_c1 | 6.15 | 1.88 | — | 1.71 | 5.74E-04 | — | 4.46E-06 | 富含半胱氨酸的类受体蛋白激酶20 Putative cysteine-rich receptor-like protein kinase 20 |
c34166.graph_c0 | 11.39 | 2.47 | — | — | 2.19E-32 | — | — | 丝氨酸/苏氨酸蛋白激酶类蛋白 CCR3 Putative serine/threonine-protein kinase-like protein CCR3 |
c33028.graph_c0 | 29.69 | 15.71 | — | — | 4.17E-24 | — | — | 丝氨酸/苏氨酸蛋白激酶PBL5 Probable serine/threonine-protein kinase PBL5 |
c33975.graph_c0 | 2.62 | 8.77 | — | 9.34 | 2.72E-07 | — | 2.25E-08 | 丝氨酸/苏氨酸蛋白激酶PIX13 Probable serine/threonine-protein kinase PIX13 |
c40710.graph_c0 | 17.50 | 8.15 | — | — | 3.24E-23 | — | — | 丝氨酸/苏氨酸蛋白激酶PBL3 Probable serine/threonine-protein kinase PBL3 |
c32569.graph_c0 | 4.53 | 11.26 | 10.70 | — | 1.63E-09 | 6.70E-21 | — | 类受体丝氨酸/苏氨酸蛋白激酶 Receptor-like serine/threonine- protein kinase |
c40773.graph_c2 | 200.52 | 48.05 | — | — | 1.71E-55 | — | — | 蛋白磷酸酶2C 25 Probable protein phosphatase 2C 25 |
c37271.graph_c0 | 25.40 | 7.32 | — | — | 1.12E-37 | — | — | 蛋白磷酸酶2C 30 Probable protein phosphatase 2C 30 |
c42044.graph_c0 | 1.49 | 0.32 | 5.29 | — | 4.45E-03 | 6.16E-10 | — | 钙结合蛋白CML31 Probable calcium-binding protein CML31 |
c32527.graph_c0 | 63.33 | 13.99 | — | 29.97 | 3.84E-53 | — | 3.08E-18 | 钙结合蛋白CML37 Calcium-binding protein CML37 |
c34433.graph_c1 | 153.82 | 61.44 | — | — | 3.46E-22 | — | — | 钙结合蛋白CML45 Probable calcium-binding protein CML45 |
c24793.graph_c0 | 29.94 | 12.63 | — | 14.07 | 3.13E-15 | — | 1.35E-07 | MYB4转录因子 Transcription factor MYB4 |
c26406.graph_c0 | 167.49 | 57.65 | — | — | 1.82E-45 | — | — | MYB4转录因子 Transcription factor MYB4 |
c35593.graph_c0 | 93.03 | 43.27 | — | — | 1.13E-27 | — | — | MYB44转录因子 Transcription factor MYB44 |
c41858.graph_c0 | 224.63 | 40.70 | — | — | 3.66E-76 | — | — | 锌指蛋白ZAT8 Zinc finger protein ZAT8 |
c23865.graph_c0 | 256.34 | 95.74 | — | 102.04 | 2.35E-43 | — | 1.07E-42 | 锌指蛋白ZAT10 Zinc finger protein ZAT10 |
c28537.graph_c0 | 264.63 | 82.51 | — | — | 3.23E-32 | — | — | 锌指蛋白ZAT10 Zinc finger protein ZAT10 |
c28249.graph_c0 | 20.67 | 3.85 | — | — | 4.70E-30 | — | — | 锌指蛋白ZAT11 Zinc finger protein ZAT11 |
c32466.graph_c0 | 164.66 | 48.15 | — | — | 2.55E-74 | — | — | RING-H2锌指蛋白ATL2 RING-H2 finger protein ATL2 |
c38319.graph_c3 | 53.94 | 16.60 | — | 26.76 | 1.79E-41 | — | 8.40E-28 | RING-H2锌指蛋白 ATL60 RING-H2 finger protein ATL60 |
图2 度尾文旦柚正常果和裂果果皮细胞壁物质含量 数据为3个生物学重复的平均值 ± 标准误差,单因素方差分析用于检测样品间的显著性差异(P < 0.05)。
Fig. 2 Cell wall material contents of the normal and cracked pericarp of fruits from the‘Duwei’pummelo cultivar Data are shown as mean±standard error of three biological replicates. Data were analyzed by using ANOVA with Duncan’s Multiple Range test(P < 0.05).
图3 实时荧光定量PCR对差异基因表达量的验证(n = 3) A. 随裂果程度的加重基因相对表达量的变化趋势;B. 基于实时荧光定量PCR和转录组测序测得的基因相对表达量的皮尔逊相关性分析。
Fig. 3 Verification of expression levels of differentially expressed genes were analyzed by quantitative real-time PCR(n = 3) A. Trends of relative expression levels of genes along with the increased fruit cracking degree;B. Pearson correlation analysis of relative expression levels of genes detected using quantitative real-time PCR and RNA-seq.
[1] |
doi: 10.3923/pjbs.2007.1611.1617 URL |
[2] |
|
[3] |
|
[4] |
doi: 10.1038/75556 pmid: 10802651 |
[5] |
doi: 10.21273/JASHS.127.3.325 URL |
[6] |
doi: 10.1093/jxb/ern234 URL |
[7] |
doi: 10.1093/jxb/eraa532 URL |
[8] |
doi: 10.1186/1471-2229-10-281 pmid: 21167067 |
[9] |
doi: 10.1186/s12870-019-1756-4 |
[10] |
doi: 10.1007/s11738-011-0862-1 URL |
[11] |
doi: 10.1186/s12870-020-02815-4 |
[12] |
|
邓泱泱, 荔建琦, 吴松锋, 朱云平, 陈耀文, 贺福初. 2006. nr 数据库分析及其本地化. 计算机工程, 32 (5):71-74.
|
|
[13] |
doi: 10.1104/pp.113.216341 URL |
[14] |
doi: 10.1093/bioinformatics/14.9.755 pmid: 9918945 |
[15] |
doi: 10.3390/genes10020163 URL |
[16] |
doi: 10.1111/tpj.v109.6 URL |
[17] |
doi: 10.1016/j.scienta.2017.12.061 URL |
[18] |
doi: 10.3390/ijms22189848 URL |
[19] |
doi: 10.3390/ijms20010068 URL |
[20] |
|
[21] |
doi: 10.1093/nar/gks1094 pmid: 23203871 |
[22] |
doi: 10.1007/s10725-016-0175-x URL |
[23] |
pmid: 8521860 |
[24] |
|
[25] |
doi: 10.1007/s42485-019-00005-x |
[26] |
doi: 10.3390/ijms19092636 URL |
[27] |
doi: 10.1007/s12011-014-0120-7 pmid: 25249068 |
[28] |
doi: 10.1074/jbc.M111.247874 pmid: 22270363 |
[29] |
|
[30] |
doi: 10.1186/s12870-019-1997-2 |
[31] |
doi: 10.1080/14620316.1999.11511120 URL |
[32] |
doi: 10.1111/ppl.2006.127.issue-2 URL |
[33] |
|
[34] |
|
贾彦丽, 智福军, 刘铁铮, 吕瑞江. 2011. 水分调控与抗蒸腾剂处理对枣裂果的影响. 河北农业科学, 15 (6):23-24,34.
|
|
[35] |
doi: 10.1038/s41438-018-0105-3 pmid: 30729007 |
[36] |
doi: 10.1016/j.plantsci.2016.03.011 pmid: 27095404 |
[37] |
|
[38] |
doi: 10.1007/s10725-004-5575-7 URL |
[39] |
doi: 10.1186/gb-2004-5-2-r7 pmid: 14759257 |
[40] |
doi: S0091-679X(20)30111-4 pmid: 32896318 |
[41] |
doi: 10.1093/bioinformatics/btt087 URL |
[42] |
doi: 10.1007/s11738-018-2785-6 |
[43] |
doi: 10.1016/j.jplph.2020.153294 URL |
[44] |
doi: 10.3389/fpls.2018.00521 URL |
[45] |
|
[46] |
doi: 10.3390/ijms151017667 pmid: 25272225 |
[47] |
|
栗现芳, 陈晓龙, 问徐鹏, 赵怡, 马辉. 2020. 枣易裂品种和抗裂品种间各糖组分含量与裂果的相关性分析. 分子植物育种, 18 (18):6180-6186.
|
|
[48] |
doi: 10.1016/j.bbrc.2017.01.104 URL |
[49] |
doi: 10.1002/jsfa.10260 pmid: 31944323 |
[50] |
doi: 10.1111/mpp.13004 URL |
[51] |
doi: 10.1006/meth.2001.1262 pmid: 11846609 |
[52] |
doi: 10.1093/jxb/erw266 URL |
[53] |
|
[54] |
|
[55] |
doi: 10.3389/fpls.2020.554520 URL |
[56] |
doi: 10.1093/bioinformatics/bti430 URL |
[57] |
|
[58] |
doi: 10.1111/ppl.2005.125.issue-2 URL |
[59] |
|
[60] |
|
[61] |
doi: 10.3389/fpls.2018.00269 URL |
[62] |
|
[63] |
doi: 10.1007/s12374-016-0442-4 URL |
[64] |
|
[65] |
|
潘周昆, 杨芯芳, 张忠鑫, 王振磊, 林敏娟. 2020. 伏脆蜜果实吸水途径及吸水后结构力学的变化. 塔里木大学学报, 32 (4):31-40.
|
|
[66] |
doi: 10.1093/pcp/pcaa009 URL |
[67] |
doi: 10.3390/plants9111557 URL |
[68] |
doi: 10.3390/ijms15022517 URL |
[69] |
doi: 10.21273/JASHS.130.4.487 URL |
[70] |
doi: 10.1111/pbi.12800 pmid: 28718511 |
[71] |
|
钱开胜. 1997. 脐橙裂果原因及其防治的初步探讨. 中国南方果树, 26 (3):12.
|
|
[72] |
doi: 10.1016/j.scienta.2015.10.037 URL |
[73] |
doi: 10.16420/j.issn.0513-353x.2017-0274 |
芮文婧, 王晓敏, 张倩男, 胡学义, 胡新华, 付金军, 高艳明, 李建设. 2018. 番茄353份种质资源表型性状遗传多样性分析. 园艺学报, 45 (3):561-570.
|
|
[74] |
doi: 10.1080/15538362.2018.1555508 URL |
[75] |
|
宋宇琴, 李洁, 付丽娇, 李娜, 李六林. 2018. ‘壶瓶枣’果实表面特征变化及其与吸水和裂果的关系. 林业科学, 54 (12):52-59.
|
|
[76] |
doi: 10.1007/s00425-013-1864-6 URL |
[77] |
|
[78] |
doi: 10.1007/s40003-019-00421-x |
[79] |
doi: 10.1038/nbt.1621 pmid: 20436464 |
[80] |
doi: 10.1093/pcp/pcn025 URL |
[81] |
|
王保明, 丁改秀, 王小原, 付春宝, 秦国杰, 杨俊强, 仓国营, 温鹏飞. 2013. 枣果实裂果的组织结构及水势变化的原因. 中国农业科学, 46 (21):4558-4568.
doi: 10.3864/j.issn.0578-1752.2013.21.019 |
|
[82] |
doi: 10.1104/pp.18.01187 pmid: 30459263 |
[83] |
doi: 10.1104/pp.20.00080 pmid: 32205452 |
[84] |
doi: 10.1093/hr/uhab027 URL |
[85] |
doi: 10.1016/j.bbrc.2018.07.030 URL |
[86] |
doi: 10.1016/j.plaphy.2021.09.026 URL |
[87] |
doi: 10.1111/pbi.12737 pmid: 28371176 |
[88] |
|
杨磊, 冯贝贝, 靳娟, 樊丁宇, 刘晶,克里木 · 伊明,郝庆. 2021. 新疆6个大果红枣裂果性差异及其内在原因分析. 西北植物学报, 41 (8):1364-1370.
|
|
[89] |
doi: 10.1007/s11103-020-01080-1 |
[90] |
doi: 10.3389/fpls.2018.01208 pmid: 30154820 |
[91] |
doi: 10.1007/s11738-016-2228-1 URL |
[92] |
|
叶正文, 叶兰香, 张学英. 2002. “朋娜”等脐橙的裂果规律及赤霉素防裂效果. 上海农业学报, 18 (4):52-57.
|
|
[93] |
doi: 10.1016/j.scienta.2019.108979 URL |
[94] |
doi: 10.1111/jipb.v63.3 URL |
[95] |
doi: 10.1007/s12374-013-0481-z URL |
[96] |
doi: 10.11869/j.issn.100-8551.2014.12.2269 |
张鹏飞, 高美英, 纪薇, 牛铁泉, 刘亚令. 2014. 叶片和果实吸水力对枣裂果的影响研究. 核农学报, 28 (12):2269-2274.
doi: 10.11869/j.issn.100-8551.2014.12.2269 |
|
[97] |
doi: 10.1007/s10126-014-9565-0 pmid: 24535704 |
[98] |
|
[1] | 代红军, 魏强, 贺琰, 汪月宁, 王振平. 油菜素内酯对高温胁迫下葡萄花色苷合成及果实品质的影响[J]. 园艺学报, 2023, 50(8): 1711-1722. |
[2] | 刘春洋, 彭朝凤, 程世平, 姚鹏强, 耿喜宁, 谢丽华. 高温诱导‘凤丹’牡丹2n雌配子创制三倍体[J]. 园艺学报, 2023, 50(7): 1455-1466. |
[3] | 阮若昕, 骆慧枫, 张琛, 黄康康, 郗笃隽, 裴嘉博, 邢梦云, 刘辉. 杭州地区不同需冷量甜樱桃品种休眠阶段花芽转录组分析[J]. 园艺学报, 2023, 50(6): 1187-1202. |
[4] | 张利军, 侯凯捷, 吴文元, 宋彪, 麦晓峰, 王玉雯, 廖文强, 李巍, 吴良泉, 李延, 郭九信. 琯溪蜜柚地上部新生器官生物量和氮磷钾养分累积动态[J]. 园艺学报, 2023, 50(6): 1318-1331. |
[5] | 王萍, 盛玲, 杨锦鹏, 周凌磊, 金燕, 罗旭钊, 马先锋, 邓子牛. 红心柚和美国枸橼杂种后代对柑橘溃疡病的抗性评价[J]. 园艺学报, 2023, 50(4): 765-777. |
[6] | 徐悦, 李子雄, 陈婕, 孙亮. 2,4-D调控番茄果形的转录基础[J]. 园艺学报, 2023, 50(4): 802-814. |
[7] | 王泽涵, 于文涛, 王鹏杰, 刘财国, 樊晓静, 谷梦雅, 蔡春平, 王攀, 叶乃兴. 茶树秃房与茸房种质花器官差异表达基因的WGCNA分析[J]. 园艺学报, 2023, 50(3): 620-634. |
[8] | 蒋彧, 涂勋良, 何俊蓉. 国兰叶色突变体叶片差异表达基因分析[J]. 园艺学报, 2023, 50(2): 371-381. |
[9] | 汤雨晴, 杨惠栋, 闫承璞, 王斯妤, 王雨亭, 胡钟东, 朱方红. 基于重测序的‘金兰柚’基因组InDel标记的开发及应用[J]. 园艺学报, 2023, 50(1): 15-26. |
[10] | 蔺海娇, 梁雨晨, 李玲, 马军, 张璐, 兰振颖, 苑泽宁. 薰衣草CBF途径相关耐寒基因挖掘与调控网络分析[J]. 园艺学报, 2023, 50(1): 131-144. |
[11] | 赵雪艳, 王琪, 王莉, 王方圆, 王庆, 李艳. 基于比较转录组的延胡索组织差异性表达分析[J]. 园艺学报, 2023, 50(1): 177-187. |
[12] | 侯潞丹, 赵梦然, 黄晨阳, 张金霞. 交替氧化酶增强糙皮侧耳高温耐受性的分子机制[J]. 园艺学报, 2022, 49(9): 1922-1934. |
[13] | 刘平, 王玖瑞, 刘志国, 王立新, 赵璇, 李建兵, 宋韬亮, 冯建华, 刘孟军. 鲜食枣新品种‘雨虹’[J]. 园艺学报, 2022, 49(8): 1833-1834. |
[14] | 刘尚佳, 吕尧, 曹逼力, 陈子敬, 高松, 徐坤. 高温渍涝胁迫对姜叶片光合作用和氮代谢的影响[J]. 园艺学报, 2022, 49(5): 1073-1080. |
[15] | 周徐子鑫, 杨威, 毛美琴, 薛彦斌, 马均. 金边红苞凤梨叶色突变体色素鉴定及类胡萝卜素合成限速基因筛选[J]. 园艺学报, 2022, 49(5): 1081-1091. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||
版权所有 © 2012 《园艺学报》编辑部 京ICP备10030308号-2 国际联网备案号 11010802023439
编辑部地址: 北京市海淀区中关村南大街12号中国农业科学院蔬菜花卉研究所 邮编: 100081
电话: 010-82109523 E-Mail: yuanyixuebao@126.com
技术支持:北京玛格泰克科技发展有限公司