萱草杂交后代及其亲本香气成分分析

doi:10.16420/j.issn.0513-353x.2022-0385

摘要/Abstract

摘要：

以萱草（Hemerocallis L.）3个杂交组合的18个优选F₁代植株及其亲本为试材，利用顶空固相微萃取结合气相色谱—质谱（HS-SPME-GC-MS）技术对其香气成分进行定性和定量测定；对决定萱草花香的两种主要香气成分(E)-β-罗勒烯和芳樟醇及香气成分总量进行分析；为明确F₁代个体香气成分的组成和含量是接近父本还是母本，对其香气成分的相对释放率做One-way SIMPER分析。结果表明：（1）3个杂交组合后代的香气成分组成和含量均与其亲本相似，母本相同时，父本的香气成分相对释放率越高，其后代香气成分的相对释放率也越高；（2）杂交亲本的主要香气成分为(E)-β-罗勒烯时，则后代个体中的(E)-β-罗勒烯含量也较高，含量和香气成分总量均介于亲本之间；杂交亲本的香气成分同时含有(E)-β-罗勒烯和芳樟醇时，则杂交后代个体中(E)-β-罗勒烯的含量可能高于亲本或与亲本接近，而芳樟醇含量介于亲本之间，香气成分总量高于两个亲本。（3）浓香型萱草野生种与没有明显香味的萱草品种杂交，能产生花香清淡的F₁代个体，是培育香花型萱草品种的良好种质资源。

关键词: 萱草, 杂交, 香气成分, HS-SPME-GC-MS

Abstract:

Eighteen F₁ hybrids from three cross combinations of Hemerocallis and their parents were used as materials，and the floral volatiles were qualitatively and quantitatively determined by headspace solid-phase extraction combined with gas chromatography-mass spectrometry（HS-SPME-GC-MS）. Relative release rate of the two main flora volatiles which decide the fragrance and the total floral volatiles of the tested materials were analyzed. One-way SIMPER analysis was performed to figure out the percentage of similarity with parents. Result showed that：（1）The composition and content of the floral volatiles of the F₁hybrids of the three hybrid combinations were similar to their parents，when the female parent is the same，the higher the relative release rate of the floral volatiles of the male parent，the higher the relative release rate of the floral volatiles of its F₁ hybrids；（2）If the main floral volatile of the hybrid parents were (E)-β-ocimene，the F₁ hybrids also contained higher amount of (E)-β-ocimene and the content of which was between the two parents，and the total amount of flora volatiles was also between the two parents；if the main flora volatiles of the parents contained both (E)-β-ocimene and linalool，the content of (E)-β-ocimene in some of the F₁ hybrids were higher than or similar to that of the parents，while the content of linalool was between the two parents，the total amount of floral volatiles was basically higher than that of the two parents；（3）Wild species with intense fragrance crossed with cultivars with no significant fragrance could breed fragrant F₁ hybrids，so they are good germplasm resources to breed fragrant daylilies.

Key words: Hemerocallis, hybridization, flora volatily, HS-SPME-GC-MS

焦芳, 孙国峰, 李丽芳, 张金政. 萱草杂交后代及其亲本香气成分分析[J]. 园艺学报, 2023, 50(7): 1495-1504.

JIAO Fang, SUN Guofeng, LI Lifang, ZHANG Jinzheng. Analysis of Flora Volatiles of the Hybrids and Their Parents in Daylily （Hemerocallis）[J]. Acta Horticulturae Sinica, 2023, 50(7): 1495-1504.

图/表 5

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化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	样品花香成分相对释放速率/（μg · h^-1· g^-1 FW） Relative emission rates of sample
化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	长者 Joan Senior	黄花菜 H. citrina	JC1	JC2	JC3	JC4	JC5	JC6
萜类化合物Terpenoids
月桂烯Myrcene	8.77	988 (988)	0.25	1.56	0.36	0.41	0.38	0.96	0.74	0.91
3-蒈烯3-Carene	9.39	1 005 (1003)	0.35	1.81	0.68	0.85	0.79	0.89	1.26	1.55
α-萜品烯α-Terpinene	10.31	1 027 (1014)	0.04	0.09	0.05	0.21	0.06	0.03	0.23	0.24
罗勒烯Ocimene	10.57	1 033 (1 027)	0.82	4.91	1.35	1.71	1.47	2.94	2.46	2.71
(E)-β-罗勒烯 (E)-β-Ocimene	11.39	1 052 (1 052)	19.51	109.17	23.75	27.48	23.72	49.68	50.49	63.15
γ-萜品烯γ-Terpinene	11.53	1 055 (1 058)	0.22	0.91	0.51	0.58	0.62	0.79	0.74	0.89
1,3-环戊二烯, 5, 5-二甲基-1-丙基 1,3-Cyclopentadiene, 5, 5-dimethyl-1-propyl-	11.97	1 066 (/)	0.05	0.29	0.12	0.16	0.15	0.24	0.24	0.29
萜品油烯Terpinolene	12.42	1 076 (1 080)	0.12	0.68	0.22	0.30	0.26	0.51	0.48	0.59
1,3,5,5-四甲基-1,3-环己二烯 1,3,5,5-Tetramethyl-1,3-cyclohexadiene	12.64	1 082 (/)	0.09	0.53	0.17	0.23	0.21	0.38	0.34	0.42
1,3-环戊二烯, 5, 5-二甲基-2-丙基 1,3-Cyclopentadiene, 5, 5-dimethyl-2-propyl-	12.88	1 087 (/)	0.11	0.59	0.25	0.34	0.30	0.54	0.52	0.60
芳樟醇Linalool	13.22	1 095 (1 100)	0	8.57	0.41	0.17	0.08	1.96	0.67	0.51
波斯菊萜Cosmene	14.11	1 116 (1 130)	0.03	0.21	0.05	0.07	0.09	0.22	0.14	0.17
4,5-环氧-反式-蒈烷 Carane, 4,5-epoxy-, trans	14.25	1 119 (/)	0.05	0	0.13	0.18	0.03	0.07	0.10	0.36
(4E,6Z)-别罗勒烯 (4E,6Z)-Allo-Ocimene	14.49	1 124 (1 126)	1.44	9.54	1.97	2.56	2.22	4.27	4.06	4.97
别罗勒烯Allo-Ocimene	14.92	1 137 (1 142)	1.02	14.46	2.14	3.08	2.54	3.51	5.86	5.07
(E)-2,6-二甲基-3,7-辛二烯-2,6-二醇 (E)-2,6-Dimethyl-3,7-octadiene-2,6-diol	15.04	1 192 (1 191)	0.05	0.03	0.13	0.17	0.02	0.06	0.09	0.36
石竹烯Caryophyllene	22.73	1 423 (1 421)	0.02	0	0	0	0	0	0	0
(E)-β-法呢烯 (E)-β-Farnesene	23.27	1 458 (1 159)	0	0.83	0.05	0.04	0.03	0.26	0.19	0.28
蛇麻烯Humulene	23.28	1 459 (1 159)	0.01	0	0	0	0	0	0	0
α-姜黄烯α-Curcumene	23.67	1 485(1 485)	0	0.07	0.01	0	0	0.04	0.02	0.02
(Z,E)-α-法呢烯 (Z,E)-α-Farnesene	23.79	1 493 (1 495)	0	2.14	0.03	0	0	0.48	0.12	0.05
α-法呢烯α-Farnesene	24.04	1 511 (1 508)	0	14.51	0.28	0.04	0.02	3.06	0.90	0.17
反式-橙花叔醇 trans-(E)-Nerolidol	24.80	1 569 (1 569)	0	4.40	0.08	0.05	0.03	0.36	0.38	0.72
部分合计Total terpenoids			24.18	175.30	32.74	38.63	33.02	71.25	70.03	84.03
部分合计/% Total terpenoids/%			99.75	95.45	99.51	97.75	94.21	99.10	98.41	98.43
苯环型化合物Benzenoids
苯乙烯Styrene	5.64	895 (898)	0.06	0.04	0	0.52	1.73	0.18	0.11	0.32
部分合计Total benzenoids			0.06	0.04	0	0.52	1.73	0.18	0.11	0.32
部分合计/%Total benzenoids/%			0.25	0.02	0	1.32	4.94	0.25	0.15	0.37
含氮化合物Nitrogenous compounds
苯乙腈Benzyl nitrile	14.92	1 135 (1 140)	0	8.01	0.16	0.37	0.30	0.47	0.80	0.88
吲哚Indole	20.25	1 290 (1 293)	0	0.31	0	0	0	0	0.22	0.14
部分合计Total nitrogenous compounds			0	8.32	0.16	0.37	0.30	0.47	1.02	1.02
部分合计/% Total nitrogenous compounds/%			0	4.53	0.49	0.94	0.86	0.65	1.43	1.19
合计Total			24.24	183.66	32.90	39.52	35.05	71.90	71.16	85.37

化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	样品花香成分相对释放速率/（μg · h^-1· g^-1 FW） Relative emission rates of sample
化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	长者 Joan Senior	黄花菜 H. citrina	JC1	JC2	JC3	JC4	JC5	JC6
萜类化合物Terpenoids
月桂烯Myrcene	8.77	988 (988)	0.25	1.56	0.36	0.41	0.38	0.96	0.74	0.91
3-蒈烯3-Carene	9.39	1 005 (1003)	0.35	1.81	0.68	0.85	0.79	0.89	1.26	1.55
α-萜品烯α-Terpinene	10.31	1 027 (1014)	0.04	0.09	0.05	0.21	0.06	0.03	0.23	0.24
罗勒烯Ocimene	10.57	1 033 (1 027)	0.82	4.91	1.35	1.71	1.47	2.94	2.46	2.71
(E)-β-罗勒烯 (E)-β-Ocimene	11.39	1 052 (1 052)	19.51	109.17	23.75	27.48	23.72	49.68	50.49	63.15
γ-萜品烯γ-Terpinene	11.53	1 055 (1 058)	0.22	0.91	0.51	0.58	0.62	0.79	0.74	0.89
1,3-环戊二烯, 5, 5-二甲基-1-丙基 1,3-Cyclopentadiene, 5, 5-dimethyl-1-propyl-	11.97	1 066 (/)	0.05	0.29	0.12	0.16	0.15	0.24	0.24	0.29
萜品油烯Terpinolene	12.42	1 076 (1 080)	0.12	0.68	0.22	0.30	0.26	0.51	0.48	0.59
1,3,5,5-四甲基-1,3-环己二烯 1,3,5,5-Tetramethyl-1,3-cyclohexadiene	12.64	1 082 (/)	0.09	0.53	0.17	0.23	0.21	0.38	0.34	0.42
1,3-环戊二烯, 5, 5-二甲基-2-丙基 1,3-Cyclopentadiene, 5, 5-dimethyl-2-propyl-	12.88	1 087 (/)	0.11	0.59	0.25	0.34	0.30	0.54	0.52	0.60
芳樟醇Linalool	13.22	1 095 (1 100)	0	8.57	0.41	0.17	0.08	1.96	0.67	0.51
波斯菊萜Cosmene	14.11	1 116 (1 130)	0.03	0.21	0.05	0.07	0.09	0.22	0.14	0.17
4,5-环氧-反式-蒈烷 Carane, 4,5-epoxy-, trans	14.25	1 119 (/)	0.05	0	0.13	0.18	0.03	0.07	0.10	0.36
(4E,6Z)-别罗勒烯 (4E,6Z)-Allo-Ocimene	14.49	1 124 (1 126)	1.44	9.54	1.97	2.56	2.22	4.27	4.06	4.97
别罗勒烯Allo-Ocimene	14.92	1 137 (1 142)	1.02	14.46	2.14	3.08	2.54	3.51	5.86	5.07
(E)-2,6-二甲基-3,7-辛二烯-2,6-二醇 (E)-2,6-Dimethyl-3,7-octadiene-2,6-diol	15.04	1 192 (1 191)	0.05	0.03	0.13	0.17	0.02	0.06	0.09	0.36
石竹烯Caryophyllene	22.73	1 423 (1 421)	0.02	0	0	0	0	0	0	0
(E)-β-法呢烯 (E)-β-Farnesene	23.27	1 458 (1 159)	0	0.83	0.05	0.04	0.03	0.26	0.19	0.28
蛇麻烯Humulene	23.28	1 459 (1 159)	0.01	0	0	0	0	0	0	0
α-姜黄烯α-Curcumene	23.67	1 485(1 485)	0	0.07	0.01	0	0	0.04	0.02	0.02
(Z,E)-α-法呢烯 (Z,E)-α-Farnesene	23.79	1 493 (1 495)	0	2.14	0.03	0	0	0.48	0.12	0.05
α-法呢烯α-Farnesene	24.04	1 511 (1 508)	0	14.51	0.28	0.04	0.02	3.06	0.90	0.17
反式-橙花叔醇 trans-(E)-Nerolidol	24.80	1 569 (1 569)	0	4.40	0.08	0.05	0.03	0.36	0.38	0.72
部分合计Total terpenoids			24.18	175.30	32.74	38.63	33.02	71.25	70.03	84.03
部分合计/% Total terpenoids/%			99.75	95.45	99.51	97.75	94.21	99.10	98.41	98.43
苯环型化合物Benzenoids
苯乙烯Styrene	5.64	895 (898)	0.06	0.04	0	0.52	1.73	0.18	0.11	0.32
部分合计Total benzenoids			0.06	0.04	0	0.52	1.73	0.18	0.11	0.32
部分合计/%Total benzenoids/%			0.25	0.02	0	1.32	4.94	0.25	0.15	0.37
含氮化合物Nitrogenous compounds
苯乙腈Benzyl nitrile	14.92	1 135 (1 140)	0	8.01	0.16	0.37	0.30	0.47	0.80	0.88
吲哚Indole	20.25	1 290 (1 293)	0	0.31	0	0	0	0	0.22	0.14
部分合计Total nitrogenous compounds			0	8.32	0.16	0.37	0.30	0.47	1.02	1.02
部分合计/% Total nitrogenous compounds/%			0	4.53	0.49	0.94	0.86	0.65	1.43	1.19
合计Total			24.24	183.66	32.90	39.52	35.05	71.90	71.16	85.37

化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	样品花香成分相对释放速率/（μg · h^-1· g^-1 FW） Relative emission rates of sample
化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	长者 Joan Senior	小黄花菜 H. minor	JM1	JM2	JM3	JM4	JM5	JM6
萜类化合物Terpenoids
3-蒈烯3-Carene	9.39	1 005 (1 003)	0.35	5.30	2.85	2.29	1.86	2.06	2.02	2.24
α-萜品烯α-Terpinene	10.31	1 027 (1 014)	0.04	0.92	1.30	1.10	1.09	1.10	1.00	0.86
罗勒烯Ocimene	10.57	1 033 (1 027)	0.82	11.39	7.56	6.50	6.53	6.42	6.33	5.89
(E)-β-罗勒烯 (E)-β-Ocimene	11.39	1 052 (1 052)	19.51	268.72	110.12	104.41	112.52	120.15	109.09	123.58
γ-萜品烯γ-Terpinene	11.53	1 055 (1 058)	0.22	3.52	4.12	3.62	3.63	3.79	3.36	3.26
1,3-环戊二烯, 5, 5-二甲基-1- 丙基 1,3-Cyclopentadiene, 5, 5-dimethyl- 1-propyl-	11.97	1 066 (/)	0.05	0.75	1.65	1.41	1.39	1.44	1.33	1.10
萜品油烯Terpinolene	12.42	1 076 (1 080)	0.12	1.81	2.50	2.17	2.18	2.28	2.07	1.83
1,3,5,5-四甲基-1,3-环己二烯 1,3,5,5-Tetramethyl-1,3- cyclohexadiene	12.64	1 082 (/)	0.09	1.30	2.15	1.82	1.82	1.88	1.68	1.50
1,3-环戊二烯, 5,5-二甲基-2- 丙基 1,3-Cyclopentadiene, 5, 5-dimethyl- 2-propyl-	12.88	1 087 (/)	0.11	1.70	3.12	2.67	2.72	2.93	2.56	2.04
芳樟醇Linalool	13.22	1 095 (1 100)	0	0.27	1.57	1.16	0	0	0	0.45
波斯菊萜Cosmene	14.11	1 116 (1 130)	0.03	0.86	0.38	0.32	0.28	0.29	0.24	0.39
4,5-环氧-反式-蒈烷 Carane, 4,5-epoxy-, trans	14.25	1 119 (/)	0.05	0.24	0.19	0.16	0.92	0.84	0.87	0.31
(4E,6Z)-别罗勒烯 (4E,6Z)-Allo-Ocimene	14.49	1 124 (1 126)	1.44	20.66	15.20	13.60	14.28	15.09	13.54	13.17
别罗勒烯Allo-Ocimene	14.92	1 137 (1 142)	1.02	12.04	20.21	18.59	19.99	23.32	17.73	21.38
(E)-2,6-二甲基-3,7-辛二烯- 2,6-二醇 (E)-2,6-Dimethyl-3,7-octadiene-2,6- diol	15.04	1 192 (1 191)	0.05	0.18	0.15	0.13	1.07	1.04	1.04	0.33
(E)-β-法呢烯 (E)-β-Farnesene	23.28	1 459 (1 457)	0	0	0.02	0.02	0.02	0.02	0.02	0.02
菖蒲二烯Acoradiene	23.48	1 472 (1 471)	0	0.14	0	0	0	0	0	0
α-姜黄烯α-Curcumene	23.67	1 485(1 485)	0	0	0.05	0.03	0.02	0.02	0.01	0.02
反式-橙花叔醇 trans-(E)-Nerolidol	24.80	1 569 (1 569)	0	0	0.02	0.03	0.02	0.02	0	0.03
部分合计Total terpenoids			23.90	329.80	173.16	160.03	170.34	182.69	162.89	178.40
部分合计/% Total terpenoids/%			99.75	94.57	96.81	97.04	96.67	97.15	97.22	93.73
苯环型化合物 Benzenoids
间二甲苯m-Xylene	5.09	870 (875)	0	0	0.20	0.11	0.05	0.05	0.13	0.41
苯乙烯Styrene	5.64	895 (898)	0.06	0.14	3.07	2.55	2.67	2.84	2.87	9.42
草酸-（2-苯乙基）二酯 Oxalic acid, di(2-phenylethyl) ester	20.38	1 295 (/)	0	1.01	0.34	0.32	0.52	0.62	0.11	0.43
部分合计Total benzenoids			0.06	1.15	3.61	2.98	3.24	3.51	3.11	10.26
部分合计/% Total benzenoids			0.25	0.33	2.02	1.81	1.84	1.87	1.86	5.39
含氮化合物 Nitrogenous compounds
苯乙腈Benzyl nitrile	14.92	1 135 (1 140)	0	1.40	1.87	1.63	2.36	1.62	1.55	1.57
吲哚Indole	20.25	1 290 (1 293)	0	16.38	0.23	0.27	0.26	0.22	0	0.11
部分合计 Total nitrogenous compounds			0	17.78	2.10	1.90	2.62	1.84	1.55	1.68
部分合计/% Total nitrogenous compounds			0	5.10	1.17	1.15	1.49	0.98	0.93	0.88
合计Total			23.96	348.73	178.87	164.91	176.20	188.04	167.55	190.34

化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	样品花香成分相对释放速率/（μg · h^-1· g^-1 FW） Relative emission rates of sample
化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	长者 Joan Senior	小黄花菜 H. minor	JM1	JM2	JM3	JM4	JM5	JM6
萜类化合物Terpenoids
3-蒈烯3-Carene	9.39	1 005 (1 003)	0.35	5.30	2.85	2.29	1.86	2.06	2.02	2.24
α-萜品烯α-Terpinene	10.31	1 027 (1 014)	0.04	0.92	1.30	1.10	1.09	1.10	1.00	0.86
罗勒烯Ocimene	10.57	1 033 (1 027)	0.82	11.39	7.56	6.50	6.53	6.42	6.33	5.89
(E)-β-罗勒烯 (E)-β-Ocimene	11.39	1 052 (1 052)	19.51	268.72	110.12	104.41	112.52	120.15	109.09	123.58
γ-萜品烯γ-Terpinene	11.53	1 055 (1 058)	0.22	3.52	4.12	3.62	3.63	3.79	3.36	3.26
1,3-环戊二烯, 5, 5-二甲基-1- 丙基 1,3-Cyclopentadiene, 5, 5-dimethyl- 1-propyl-	11.97	1 066 (/)	0.05	0.75	1.65	1.41	1.39	1.44	1.33	1.10
萜品油烯Terpinolene	12.42	1 076 (1 080)	0.12	1.81	2.50	2.17	2.18	2.28	2.07	1.83
1,3,5,5-四甲基-1,3-环己二烯 1,3,5,5-Tetramethyl-1,3- cyclohexadiene	12.64	1 082 (/)	0.09	1.30	2.15	1.82	1.82	1.88	1.68	1.50
1,3-环戊二烯, 5,5-二甲基-2- 丙基 1,3-Cyclopentadiene, 5, 5-dimethyl- 2-propyl-	12.88	1 087 (/)	0.11	1.70	3.12	2.67	2.72	2.93	2.56	2.04
芳樟醇Linalool	13.22	1 095 (1 100)	0	0.27	1.57	1.16	0	0	0	0.45
波斯菊萜Cosmene	14.11	1 116 (1 130)	0.03	0.86	0.38	0.32	0.28	0.29	0.24	0.39
4,5-环氧-反式-蒈烷 Carane, 4,5-epoxy-, trans	14.25	1 119 (/)	0.05	0.24	0.19	0.16	0.92	0.84	0.87	0.31
(4E,6Z)-别罗勒烯 (4E,6Z)-Allo-Ocimene	14.49	1 124 (1 126)	1.44	20.66	15.20	13.60	14.28	15.09	13.54	13.17
别罗勒烯Allo-Ocimene	14.92	1 137 (1 142)	1.02	12.04	20.21	18.59	19.99	23.32	17.73	21.38
(E)-2,6-二甲基-3,7-辛二烯- 2,6-二醇 (E)-2,6-Dimethyl-3,7-octadiene-2,6- diol	15.04	1 192 (1 191)	0.05	0.18	0.15	0.13	1.07	1.04	1.04	0.33
(E)-β-法呢烯 (E)-β-Farnesene	23.28	1 459 (1 457)	0	0	0.02	0.02	0.02	0.02	0.02	0.02
菖蒲二烯Acoradiene	23.48	1 472 (1 471)	0	0.14	0	0	0	0	0	0
α-姜黄烯α-Curcumene	23.67	1 485(1 485)	0	0	0.05	0.03	0.02	0.02	0.01	0.02
反式-橙花叔醇 trans-(E)-Nerolidol	24.80	1 569 (1 569)	0	0	0.02	0.03	0.02	0.02	0	0.03
部分合计Total terpenoids			23.90	329.80	173.16	160.03	170.34	182.69	162.89	178.40
部分合计/% Total terpenoids/%			99.75	94.57	96.81	97.04	96.67	97.15	97.22	93.73
苯环型化合物 Benzenoids
间二甲苯m-Xylene	5.09	870 (875)	0	0	0.20	0.11	0.05	0.05	0.13	0.41
苯乙烯Styrene	5.64	895 (898)	0.06	0.14	3.07	2.55	2.67	2.84	2.87	9.42
草酸-（2-苯乙基）二酯 Oxalic acid, di(2-phenylethyl) ester	20.38	1 295 (/)	0	1.01	0.34	0.32	0.52	0.62	0.11	0.43
部分合计Total benzenoids			0.06	1.15	3.61	2.98	3.24	3.51	3.11	10.26
部分合计/% Total benzenoids			0.25	0.33	2.02	1.81	1.84	1.87	1.86	5.39
含氮化合物 Nitrogenous compounds
苯乙腈Benzyl nitrile	14.92	1 135 (1 140)	0	1.40	1.87	1.63	2.36	1.62	1.55	1.57
吲哚Indole	20.25	1 290 (1 293)	0	16.38	0.23	0.27	0.26	0.22	0	0.11
部分合计 Total nitrogenous compounds			0	17.78	2.10	1.90	2.62	1.84	1.55	1.68
部分合计/% Total nitrogenous compounds			0	5.10	1.17	1.15	1.49	0.98	0.93	0.88
合计Total			23.96	348.73	178.87	164.91	176.20	188.04	167.55	190.34

化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	样品花香成分相对释放速率/（μg · h^-1· g^-1 FW） Relative emission rates of sample
化合物名称 Compound name	保留时间/ min Retention time	保留指数 Retention index	奋进 On and On	大苞萱草 H. middendorfii	OM1	OM2	OM3	OM4	OM5	OM6
萜类化合物Terpenoids
月桂烯Myrcene	8.77	988 (988)	0.54	2.49	6.40	13.63	17.57	10.89	12.95	3.16
3-蒈烯3-Carene	9.39	1 005 (1 003)	0.76	1.78	7.81	7.58	5.22	5.48	5.78	7.60
α-萜品烯α-Terpinene	10.31	1 027 (1 014)	0.12	0	0	2.59	2.16	2.08	1.57	1.92
罗勒烯Ocimene	10.57	1 033 (1 027)	1.62	4.17	6.76	7.30	5.89	5.70	5.81	8.81
(E)-β-罗勒烯(E)-β-Ocimene	11.39	1 052 (1 052)	49.21	56.55	47.22	88.92	80.92	87.48	97.90	114.11
γ-萜品烯γ-Terpinene	11.53	1 055 (1 058)	0.45	1.41	2.80	3.24	2.64	2.91	3.07	4.61
1,3-环戊二烯, 5,5-二甲基- 1-丙基 1,3-Cyclopentadiene, 5,5- dimethyl-1-propyl-	11.97	1 066 (/)	0.11	0.23	1.73	2.40	1.42	1.59	1.63	1.99
萜品油烯Terpinolene	12.42	1 076 (1 080)	0.26	0.54	2.15	3.31	2.01	2.16	2.25	2.77
1,3,5,5-四甲基-1,3-环己二烯 1,3,5,5-Tetramethyl-1,3-cyclohexadiene	12.64	1 082 (/)	0.19	0.84	6.85	3.13	2.02	2.04	2.05	2.71
1,3-环戊二烯, 5,5-二甲基- 2-丙基 1,3-Cyclopentadiene, 5,5- dimethyl-2-propyl-	12.88	1 087 (/)	0.21	0.47	2.09	4.32	2.65	2.75	2.80	3.43
芳樟醇Linalool	13.22	1 095 (1 100)	0	53.16	27.82	10.63	18.97	14.71	12.47	8.62
波斯菊萜Cosmene	14.11	1 116 (1 130)	0.09	0.33	0.98	0.36	0.29	0.40	0.43	0.49
4,5-环氧-反式-蒈烷 Carane, 4,5-epoxy-, trans	14.25	1 119 (/)	0.04	0.59	0.18	0.36	0.46	0	0	0.63
(4E,6Z)-别罗勒烯 (4E,6Z)-Allo-Ocimene	14.49	1 124 (1 126)	3.20	5.65	12.90	16.11	11.85	12.44	13.22	16.84
别罗勒烯Allo-Ocimene	14.92	1 137 (1 142)	2.20	5.63	15.77	22.70	21.68	19.37	20.28	21.37
(E)-2,6-二甲基-3,7-辛二烯- 2,6-二醇 (E)-2,6-Dimethyl-3,7- octadiene-2,6-diol	15.04	1 192 (1 191)	0.04	0.58	0.40	0.44	0.62	0.05	0.03	0.60
石竹烯Caryophyllene	22.73	1 423 (1 421)	0.01	0.22	0	0.07	0	0	0	0
(E)-β-法呢烯 (E)-β-Farnesene	23.27	1 458 (1 159)	0.02	0.10	0	0	0.07	0	0.05	0
α-姜黄烯α-Curcumene	23.67	1 485(1 485)	0.01	0	0.04	0.04	0.15	0.14	0.08	0.06
(Z,E)-α-法呢烯 (Z,E)-α-Farnesene	23.79	1 493 (1 495)	0.08	0.59	0.03	0.05	0.17	0	0	0
α-法呢烯α-Farnesene	24.04	1 511 (1 508)	1.04	5.23	0.24	0.56	1.57	0.08	0.07	0.88
反式-橙花叔醇trans-(E)-Nerolidol	24.80	1 569 (1 569)	0.05	0.16	0	0.02	0.07	0.05	0.03	0.04
部分合计Total terpenoids			60.25	140.72	142.17	187.76	178.40	170.32	182.47	200.64
部分合计/% Total terpenoids			98.40	94.71	97.16	97.11	97.30	97.28	97.18	97.72
苯环型化合物 Benzenoids
间二甲苯m-Xylene	5.09	870 (875)	0.01	0.86	0.16	0.14	0.02	0.05	0.23	0.24
苯乙烯Styrene	5.64	895 (898)	0.12	2.64	0.68	0.89	0.52	1.05	1.12	1.19
苯乙醇Phenethyl alcohol	13.79	1 108 (1 109)	0.24	0	0.69	0.65	0.79	0.46	0.51	0.48
草酸-(2-苯乙基)二酯 Oxalic acid, di(2-phenylethyl) ester	20.38	1 295 (/)	0.17	0	0.38	0.50	0.60	0.63	0.73	0.38
部分合计Total benzenoids			0.54	3.50	1.91	2.18	1.93	2.19	2.59	2.29
部分合计/% Total benzenoids			0.88	2.36	1.31	1.13	1.05	1.25	1.38	1.12
含氮化合物 Nitrogenous compounds
苯乙腈Benzyl nitrile	14.92	1 135 (1 140)	0	1.80	1.96	2.99	2.14	2.15	2.15	2.25
吲哚Indole	20.25	1 290 (1 293)	0.44	2.56	0.29	0.42	0.89	0.43	0.56	0.14
部分合计 Total nitrogenous compounds			0.44	4.36	2.25	3.41	3.02	2.58	2.71	2.39
部分合计/% Total nitrogenous compounds			0.72	2.93	1.54	1.76	1.65	1.47	1.44	1.16
合计Total			61.23	148.58	146.33	193.35	183.35	175.09	187.77	205.32